In this study, the thermophysiological comfort and objective hand properties of modal, Naia™, cashmere, and cotton containing denim fabrics were investigated. Therefore, the air permeability, thermal conductivity, thermal absorptivity, thermal resistance, water vapor permeability, overall flexural rigidity, and compressibility of the fabrics were examined. It was concluded that using Naia™ sustainable fiber improved the thermal comfort and objective hand properties of denim fabrics. The elastane containing fabrics (T5 and T6) were uncomfortable because of their lower air permeability, lower water vapor permeability and higher overall flexural rigidity properties. Sulfur dyeing only affects the thermal resistance of fabrics, and the thermal resistance of sulfur dyed fabrics is higher than that of indigo dyed fabrics with the same compositions.