A novel flow‐injection chemiluminescence system for the determination of guanine

Abstract

A novel flow-injection chemiluminescence (CL) method for the determination of guanine was developed. The procedure is based on the CL reaction of guanine with hydrogen peroxide in borax buffer (pH 8.5) with Co2+ as a catalyst. The calibration graph is linear within the range of 3 x 10(-7)-9 x 10(-5) g/mL. A detection limit of 1 x 10(-7) g/mL, along with a relative standard deviation of 2.23% (3 x 10(-7) g/mL guanine, n = 11), were obtained. The present procedure was applied to the measurement of guanine in urine with recoveries of 97.5-107.5%. A possible CL mechanism of the reaction system is proposed.

Facile aqueous synthesis of functionalized CdTe nanoparticles and their application as fluorescence probes for determination of adenine and guanine

“…25 However, each approach exhibits certain disadvantages. For example, chromatographic methods often require the isolation of matrix components, as well as derivatization steps, 26 while the electrochemical technique tends to suffer from irreversible adsorption on the electrode surface thus hampering the estimation. 19,27 In contrast, the fluorescence probe method appears to be a simple and fast experimental process, with high sensitivity for different fluorescent emission wavelengths corresponding to different molecular structures.…”

Fabrication of Cu−CeO2 Coated Multiwall Carbon Nanotube Composite Electrode for Simultaneous Determination of Guanine and Adenine

“…Therefore; simple, cheap and fast analytical methods are needed for sensitive and selective determination of these two bases. For this purpose, various analytical methods such as high performance liquid chromatography , laser induced fluorescence detection , capillary zone electrophoresis , chemiluminescence , spectroscopic methods and mass spectrometry have been employed for the determination of guanine and adenine in recent years.…”

Electrochemical sensing of guanine, adenine and 8-hydroxy-2′-deoxyguanosine at glassy carbon modified with single-walled carbon nanotubes covalently functionalized with lysine

“…19 Therefore, the development of sensitive and selective methodologies for their quantication are very important not only to understand basic aspects of DNA damage, hybridization and protein-DNA interaction 20,21 but also in Physiology and Clinical Pathology. 22 Several methodologies have been reported for the quantication of guanine and adenine involving chromatographic [23][24][25][26] and spectrometric 27,28 methods. However, although these techniques offer high sensitivity and selectivity, they present some disadvantages like complex sample preparation, use of sophisticated instruments, and requirement of skilled personnel that restrict their routine use.…”