A novel HPQ-based fluorescent probe for the visualization of carbon monoxide in zebrafish

Strategy for Detecting Carbon Monoxide: Cu2+-Assisted Fluorescent Probe and Its Applications in Biological Imaging

“…Tricarbonylchloro (glycinato) ruthenium­(II) (CORM-3) was used as the source of CO. − Intriguingly, when adding CO to the solution of DPHP + Cu 2+ in succession, the fluorescent intensity at 590 was recovered to the former level (Figure c). It is assumed that CO might have deoxidized Cu 2+ to Cu + , but Cu + could not interact with DPHP , thus leading to the recovered intensity of 590 nm.…”

“…Traditional methods, such as absorption spectroscopy, chromatography, and electrochemical analysis, might destroy the tested sample. Besides, they continue to fall short of the demands like the real time or the low-cost. − Endowed with many superiorities, fluorescent probes have recently been attested to be robust methods to recognize CO. − Currently, numerous fluorescent probes are found to effectively interact with CO based on the organic palladium complex, Pd 0 -mediated Tsuji–Trost reaction, − and nitro reduction reaction. ,, …”

“…Among them, Tsuji–Trost reaction catalyzed by Pd 0 is the most extensively studied due to its rapid reaction and easy accessibility. − When Tsuji–Trost reaction occurs, Pd 2+ is reduced to Pd 0 by CO. At the same time, the π-allyl complex is developed between Pd 0 and the allyl group . After the nucleophile attacks the complex, the specific group is left .…”

Golgi-Targeting Fluorescent Probe for Monitoring CO-Releasing Molecule-3 In Vitro and In Vivo

“…Therefore, in the past decades, the development of appropriate CO-releasing molecules (CORMs) to replace CO gas for direct treatment of diseases has received extensive attention . So far, many methods for detecting CO using CORM-3 as a CO donor have been developed, including chromogenic detection, electrochemical analysis, gas chromatography, and fluorescent assays. − Among these, because of its high sensitivity, imperial spatial resolution, noninvasive detection, technical simplicity, etc., fluorescent imaging has special advantage in monitoring the spatial and temporal distribution of analytes. − Recently, numerous innovative fluorescent probes have been reported for the visualization of CO using CORM-3 as a CO donor. − …”

Turn-on silicon-based fluorescent probe for visualizing endogenous CO during hypoxia

“…Fluorescent probes have the ability to quickly enter cells in a non-invasive manner, have high sensitivity and excellent photostability, and have become one of the main methods for studying biological processes. 32–35 The Tsuji–Trost reaction using CO reduction has always been the main design idea behind CO florescent probes responding to CO. 36–46 For example, Feng's group designed a fluorescent probe that uses allyl chloroformate to protect its amino group and restores fluorescence by reducing Pd 2+ with CO. 47 As a heavy metal element, palladium is inevitably toxic to organisms, which is not conducive to the visualization and tracking of endogenous CO. The critical issue for this target is the lack of related CO probe design strategies.…”