A novel hydrogen peroxide biosensor based on Au–graphene–HRP–chitosan biocomposites

Zhou, Kangfu; Zhu, Yihua; Yang, Xin; Luo, Jie; Li, Chunzhong; Luan, Shaorong

doi:10.1016/j.electacta.2010.01.035

Cited by 364 publications

(150 citation statements)

References 51 publications

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“…[10][11][12] Here, dopamine was used as a model assay to reveal the characterization of G-CFM. Figure 7A shows the voltammograms of 0.5 mM DA at CFM and G-CFM in 0.1 M pH 7.0 phosphate buffer solution (PBS).…”

Section: Electrochemical Behavior Of Da At G-cfmmentioning

confidence: 99%

“…Some groups have studied DA using carbon fiber microelectrodes modified by carbon nanomaterials, such as single-walled or multiwalled carbon nanotubes. [12][13][14][15][16] Graphene, as a novel one-atom thick and two-dimensional graphitic carbon system, exhibits superior electrical conductivity and high specific surface area. Recently, much advancement has been achieved by using graphene in electrochemical applications, even electrochemical sensors or devices.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of Graphene Coated Carbon Fiber Microelectrode for Highly Sensitive Detection Application

et al. 2014

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Graphene, as a novel carbon nanomaterial, exhibits superior performance in electrochemical sensors. Here, graphene was applied to the microelectrode system by a simple method. A novel graphene coating carbon fiber microelectrode (G-CFM) was fabricated by electrodepositing graphene on the surface of carbon fiber. The fabrication method is fast and simple. Scanning electron microscopy and Raman spectroscopy demonstrated that carbon fiber was successfully modified by graphene. The electrochemical behavior of G-CFM was characterized by potassium ferricyanide and dopamine (DA). The electrode exhibited much larger current response and less overpotential response, compared to CFM. The microsensor for DA showed good sensitivity and selectivity, and the electrode had good stability. It is believable that the unique characteristic of graphene holds promise for the advanced microelectrode system for highly sensitive detection of various targets.

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Section: Electrochemical Behavior Of Da At G-cfmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of Graphene Coated Carbon Fiber Microelectrode for Highly Sensitive Detection Application

et al. 2014

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“…As such, it serves as a matrix for the assembly of biomolecules, nanoparticles and other substances [27]. Combinations of chitosan with graphene or metal/metal oxide nanoparticles have also been tested as the electrochemical biosensing platforms [25][26]. In this paper, the construction of a new amperometric xanthine biosensor for fish freshness detection based on immobilization of xanthine oxidase on Nafion/Co3O4/CH/GR composite film modified GC is presented.…”

Section: Introductionmentioning

confidence: 99%

“…Among various types of nanoparticles, Co3O4 nanoparticles are of special interest in recent years as the material is cheap, biocompatible, easily available, and has highly prospective electrocatalytic properties [21]. Up to now, various metal or metal oxide nanoparticles and graphene composites such as Pd−graphene [22], Fe3O4−graphene oxide [23], TiO2-graphene [24], Pt nanoparticles−graphene [25], and Au-graphene [26] have been investigated for biosensor construction and synergistic effects of these composites in electrocatalytic applications have been reported. With its extraordinary properties such as film forming capability, superior mechanical strength, high permeableness, and nontoxicity, chitosan…”

Section: Introductionmentioning

confidence: 99%

Construction of an Electrochemical Xanthine Biosensor Based on Graphene/Cobalt Oxide Nanoparticles/Chitosan Composite for Fish Freshness Detection

Dalkıran¹,

Erden²,

Kılıç³

2016

J. Turk. Chem. Soc., Sect. A: Chem.

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Abstract:A xanthine biosensor based on glassy carbon electrode (GC) modified with graphene (GR), Co3O4 nanoparticles, and chitosan (CH) composite was fabricated. Xanthine oxidase (XAO) solution was dropped on the surface of Nafion/Co3O4/CH/GR/GC and the electrode was placed in saturated glutaraldehyde vapor for the crosslinking of the enzymes. The modified electrode was characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Under the optimized experimental conditions, xanthine was detected in the concentration range from 5.0×10 -4 to 8.0×10-2 mM with a detection limit of 2.0×10 -4 mM. The low Michaelis-Menten constant (0.17 mM) suggested enhanced enzymic affinity for the immobilized enzyme as compared to previously reported xanthine biosensors. Moreover, the biosensor exhibited some advantages, such as short response time (10 s), high sensitivity (6.58 μA/mM or 74.8 µA/mMcm 2 ), and good reproducibility (RSD = 1.2%). The suitability of the device was verified by xanthine assay in a fish meat sample. The biosensor provided to be a reliable, easy, fast and economical method for the evaluation of fish freshness.

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“…High concentrations of H 2 O 2 would cause irritation to dyes and shin, it affects human health [2]. On the other hand, the determination of H 2 O 2 concentration is important in food [3], clinical [4] and environmental applications [5]. There are many methods which can be used for the quantitative analysis of H 2 O 2 in solution, such as electrochemical method [6] and spectroscopy [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide sensing based on carbon quantum dots

Chu

Hsieh

2016

MATEC Web of Conferences

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Abstract. In this work, carbon quantum dots (CQDs) have been synthesized by one step hydrothermal method to detect hydrogen peroxide (H2O2). The optical H2O2 sensors were based on the fluorescent quenching of the carbon quantum dots. The optical H2O2 sensors demonstrate capable of detecting H2O2 over the range of 0-88.2 mM and 0-60 mM. The H2O2 sensitivities of wavelength shift to changes in the H2O2 concentration were found to be 0.18 nm/mM and 0.26 nm/mM. These results of the optical sensors showed that the method can be used in practice detection of H2O2 and could offer a new approach for developing a new optical biosensor. The CQDs exhibit good emission property and high stability, as well as excitation-independent emission behavior. Moreover, it is attractive that CQDs can be used as an effective fluorescent probe for the detection of H2O2 with linear Stern-Volmer plot and wavelength shift in an aqueous solution.

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A novel hydrogen peroxide biosensor based on Au–graphene–HRP–chitosan biocomposites

Cited by 364 publications

References 51 publications

Fabrication of Graphene Coated Carbon Fiber Microelectrode for Highly Sensitive Detection Application

Fabrication of Graphene Coated Carbon Fiber Microelectrode for Highly Sensitive Detection Application

Construction of an Electrochemical Xanthine Biosensor Based on Graphene/Cobalt Oxide Nanoparticles/Chitosan Composite for Fish Freshness Detection

Hydrogen peroxide sensing based on carbon quantum dots

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