A novel insulin microcrystals preparation using a seed zone method

Microcrystallization of α-lactalbumin

“…[19]. In addition, in the pharmaceutical field micron-sized crystals are applicable for poorly water-soluble drugs [20], suspension injection [21], and pulmonary inhalation delivery [22][23][24]. The crystallinity of the spherical microcrystals which we obtained was investigated by the X-ray diffractometry and differential scanning calorimetry.…”

Photolysis of Recombinant Human Insulin in the Solid State: Formation of a Dithiohemiacetal Product at the C-Terminal Disulfide Bond

“…The microcrystals were prepared using a previously described method (40,41). Crystalline human insulin powders from Roche and Millipore, respectively, were completely dissolved in acetic acid (0.1 N) in the presence of zinc sulfate to reach a concentration of 1 mg/mL of insulin containing 0.4% (w/w) zinc ions.…”

Crystallization of Antiangiogenic Kringle V Derived from Human Apolipoprotein A: Crystallization Applied to Purification and Formulation

“…[12][13][14] Normally, though, the crystallization process is rarely applied to protein purification; because protein crystals are quite difficult to prepare on a large scale, the preparation normally takes quite a bit of time. 15) In addition to the advantages noted with regard to the purification procedure, the crystal form of apolipoprotein A kringle V was expected to manifest additional advantages as a formulation, rendering storage at high concentrations and large-dosage administration more convenient.…”