A novel iron quantum cluster confined in hemoglobin as fluorescent sensor for rapid detection of Escherichia coli

Vaezi, Zahra; Azizi, Mina; Mohammadi, Sanam Sadeghi; Hashemi, Naimeh; Naderi-Manesh, Hossein

doi:10.1016/j.talanta.2020.121137

Cited by 13 publications

(5 citation statements)

References 46 publications

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“…Besides the wide range of therapeutic-oriented usages presented above, inorganic NPs can also be considered as valuable diagnostic tools. In this regard, Vaezi et al [ 193 ] showed in a recent study the possibilities of using an effective and inexpensive NP-based diagnosis method for urinary tract infections [ 193 ]. Other studies have shown the application of different metal quantum clusters, which have fluorescent properties.…”

Section: Nanotechnology Used As a Diagnostic Methods And In Antimicro...mentioning

confidence: 99%

“…Therefore, it would be an easy method of identifying Escherichia coli strains in a rapid and effective manner even at a low Escherichia coli concentration of only 8.3 × 103 CFU/mL. The Hb-FeQCs are also water-soluble, stable, biocompatible, and highly sensitive to bacterial concentrations, especially in the urine medium [ 193 , 194 , 195 ].…”

Section: Nanotechnology Used As a Diagnostic Methods And In Antimicro...mentioning

confidence: 99%

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Nanotechnology Involved in Treating Urinary Tract Infections: An Overview

et al. 2023

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Considered as the most frequent contaminations that do not require hospitalization, urinary tract infections (UTIs) are largely known to cause significant personal burdens on patients. Although UTIs overall are highly preventable health issues, the recourse to antibiotics as drug treatments for these infections is a worryingly spread approach that should be addressed and gradually overcome in a contemporary, modernized healthcare system. With a virtually alarming global rise of antibiotic resistance overall, nanotechnologies may prove to be the much-needed ‘lifebuoy’ that will eventually suppress this prejudicial phenomenon. This review aims to present the most promising, currently known nano-solutions, with glimpses on clinical and epidemiological aspects of the UTIs, prospective diagnostic instruments, and non-antibiotic treatments, all of these engulfed in a comprehensive overview.

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Section: Nanotechnology Used As a Diagnostic Methods And In Antimicro...mentioning

confidence: 99%

Section: Nanotechnology Used As a Diagnostic Methods And In Antimicro...mentioning

confidence: 99%

Nanotechnology Involved in Treating Urinary Tract Infections: An Overview

et al. 2023

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“…While E. coli can be detected in urine using iron quantum clusters by interacting with Cu from the bacteria, Zinc-Cobalt ferrite nanoparticles may be used as an antimicrobial agent [79]. Establishing the microorganism causing sepsis is often a race against time and can prove invaluable in critically ill patients [88]. In oncology, magnetic Concanavalin A-NPs have been used to detect Cathepsin C and transferrin, which may serve as biomarkers in the urine of small-cell lung cancer patients [89].…”

Section: Biological Sample Analysismentioning

confidence: 99%

Iron, Copper, and Zinc Homeostasis: Physiology, Physiopathology, and Nanomediated Applications

2021

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Understanding of how the human organism functions has preoccupied researchers in medicine for a very long time. While most of the mechanisms are well understood and detailed thoroughly, medicine has yet much to discover. Iron (Fe), Copper (Cu), and Zinc (Zn) are elements on which organisms, ranging from simple bacteria all the way to complex ones such as mammals, rely on these divalent ions. Compounded by the continuously evolving biotechnologies, these ions are still relevant today. This review article aims at recapping the mechanisms involved in Fe, Cu, and Zn homeostasis. By applying the knowledge and expanding on future research areas, this article aims to shine new light of existing illness. Thanks to the expanding field of nanotechnology, genetic disorders such as hemochromatosis and thalassemia can be managed today. Nanoparticles (NPs) improve delivery of ions and confer targeting capabilities, with the potential for use in treatment and diagnosis. Iron deficiency, cancer, and sepsis are persisting major issues. While targeted delivery using Fe NPs can be used as food fortifiers, chemotherapeutic agents against cancer cells and microbes have been developed using both Fe and Cu NPs. A fast and accurate means of diagnosis is a major impacting factor on outcome of patients, especially when critically ill. Good quality imaging and bed side diagnostic tools are possible using NPs, which may positively impact outcome.

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“…excellent uorescence, water solubility and biocompatibility). Fluorescence methods for E. coli have been widely studied for their high sensitivity and simplicity [5] . Biosensor technology is widely used for pathogenic bacteria detection.…”

Section: Introductionmentioning

confidence: 99%

“…The folding of aptamers with intramolecular interactions leads to secondary and tertiary conformations with high speci city for their targets. Aptamers have good stability in different organic solvents, temperatures and pH (4)(5)(6)(7)(8)(9), have the exibility to be used as biosensors, are easy to synthesize by the SELEX process and can be chemically modi ed and coupled [6] . Other advantages of aptamers compared to antibodies are as follows: aptamers are highly stable at high temperatures and in long-term storage.…”

Section: Introductionmentioning

confidence: 99%

Based on Aptamer-carbon quantum dots and silver nanoparticles FRET sensor for sensitive detection of E. coli

Bai¹,

Ai³

2023

Preprint

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In this work, carbon quantum dots were prepared from grapefruit peel as carbon source by microwave heating method. The prepared carbon quantum dots CQDs were analyzed by fluorescence spectroscopy, TEM analysis, XPS analysis, etc., as well as their optical properties were also investigated. The prepared CQDs have high green fluorescence with excitation wavelength of 320 nm and maximum emission wavelength of 415 nm. the average particle size is about 7.4 nm, uniform dispersion and good stability. And a FRET (fluorescence resonance energy transfer) based fluorescence method was constructed by combining carbon quantum dots with silver nanoparticles (Ag NPs) for the rapid detection of E. coli. Its fluorescence transduction is based on the spectral overlap between the donor (CQDs) emission and the acceptor (nanoparticles) absorbance. The fluorescence of the aptamer-attached CQDs is burst in the presence of and silver nanoparticles. Upon addition of the specific E. coli solution, an aptamer-target complex is formed and the preferential interaction of the aptamer with the specific bacteria leads to the release of CQDs and Ag NPs. After incubation time, the bacterial cells are centrifuged, leading to the precipitation of E. coli aptamer couples and Ag NPs, resulting in the recovery of CQDs fluorescence. This method allows specific detection of E. coli in a wide range of pathogenic bacteria. The final results showed that the linear range of the sensor was 2×103 ~ 2×108 CFU·mL− 1 and the detection limit for E. coli was as low as 77 CFU·mL− 1.

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A novel iron quantum cluster confined in hemoglobin as fluorescent sensor for rapid detection of Escherichia coli

Cited by 13 publications

References 46 publications

Nanotechnology Involved in Treating Urinary Tract Infections: An Overview

Nanotechnology Involved in Treating Urinary Tract Infections: An Overview

Iron, Copper, and Zinc Homeostasis: Physiology, Physiopathology, and Nanomediated Applications

Based on Aptamer-carbon quantum dots and silver nanoparticles FRET sensor for sensitive detection of E. coli

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