A novel label-free electrochemical impedance aptasensor for highly sensitive detection of human interferon-gamma based on target-induced exonuclease inhibition

Li, Huan; Song, Shihao; Wen, Meiqi; Bao, Ting; Wu, Zhen; Xiong, Huayu; Zhang, Xun; Wen, Wei

doi:10.1016/j.bios.2019.111532

Cited by 31 publications

(23 citation statements)

References 36 publications

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“…Impedance sensors have been deployed for a variety of protein targets, using a frequency sweep at a fixed potential (electrochemical impedance spectroscopy, EIS) [13][14][15] as well as a voltage sweep at a fixed frequency (differential pulse voltammetry (DPV) or square-wave voltammetry (SWV)) [16]. However, implementation and optimization of these sensors remains challenging due to non-specific binding, sensor drift and other factors [17] for all impedimetric sensors, particularly those using EIS or SWV measurements, with either the redox probe in solution or immobilized onto a BRE.…”

Section: Introductionmentioning

confidence: 99%

Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Brothers

Moore

Lawrence

et al. 2020

Sensors

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Real-time sensing of proteins, especially in wearable devices, remains a substantial challenge due to the need to convert a binding event into a measurable signal that is compatible with the chosen analytical instrumentation. Impedance spectroscopy enables real-time detection via either measuring electrostatic interactions or electron transfer reactions while simultaneously being amenable to miniaturization for integration into wearable form-factors. To create a more robust methodology for optimizing impedance-based sensors, additional fundamental studies exploring components influencing the design and implementation of these sensors are needed. This investigation addresses a sub-set of these issues by combining optical and electrochemical characterization to validate impedance-based sensor performance as a function of (1) biorecognition element density, (2) self-assembled monolayer chain length, (3) self-assembled monolayer charge density, (4) the electrochemical sensing mechanism and (5) the redox reporter selection. Using a pre-existing lysozyme aptamer and lysozyme analyte combination, we demonstrate a number of design criteria to advance the state-of-the-art in protein sensing. For this model system we demonstrated the following: First, denser self-assembled monolayers yielded substantially improved sensing results. Second, self-assembled monolayer composition, including both thickness and charge density, changed the observed peak position and peak current. Third, single frequency measurements, while less informative, can be optimized to replace multi-frequency measurements and in some cases (such as that with zwitterionic self-assembled monolayers) are preferred. Finally, various redox reporters traditionally not used in impedance sensing should be further explored. Collectively, these results can help limit bottlenecks associated with device development, enabling realization of next-generation impedance-based biosensing with customize sensor design for the specific application.

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Section: Introductionmentioning

confidence: 99%

Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Brothers

Moore

Lawrence

et al. 2020

Sensors

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“…In other words, impedance was larger on the electrode surface. The proposed aptasensor achieved a low detection limit of 0.7 pM over a wide range of 1 pM to 50 nM (Figure 3d) [51].…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 97%

“…Li group developed a label-free electrochemical impedance aptasensor based on target-induced exonuclease (Exo) inhibition to detect IFN-γ [51] (Figure 3c). IFN-γ was detected using a DNA hairpin with a loop of IFN-γ aptamer and a stem consisting of 5 -thiol modified as a probe.…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Recent Advances in Aptasensor for Cytokine Detection: A Review

Kim

Noh

Park

et al. 2021

Sensors

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Cytokines are proteins secreted by immune cells. They promote cell signal transduction and are involved in cell replication, death, and recovery. Cytokines are immune modulators, but their excessive secretion causes uncontrolled inflammation that attacks normal cells. Considering the properties of cytokines, monitoring the secretion of cytokines in vivo is of great value for medical and biological research. In this review, we offer a report on recent studies for cytokine detection, especially studies on aptasensors using aptamers. Aptamers are single strand nucleic acids that form a stable three-dimensional structure and have been receiving attention due to various characteristics such as simple production methods, low molecular weight, and ease of modification while performing a physiological role similar to antibodies.

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“…Although they achieved good results, they had difficulty in designing the THMS system for the aptamer to identify its target efficiently. In another study, Li et al [ 59 ] developed a label-free impedance aptasensor that was based on target-induced exonuclease inhibition. The electron transfer resistance ( R ct ) increased when interferon-gamma was added, because of the double exonuclease reaction taking place.…”

Section: Sensor Detection Techniquesmentioning

confidence: 99%

Section: Sensor Detection Techniquesmentioning

confidence: 99%

Recent advances in the detection of interferon-gamma as a TB biomarker

et al. 2021

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Tuberculosis (TB) is one of the main infectious diseases worldwide and accounts for many deaths. It is caused by Mycobacterium tuberculosis usually affecting the lungs of patients. Early diagnosis and treatment are essential to control the TB epidemic. Interferon-gamma (IFN-γ) is a cytokine that plays a part in the body’s immune response when fighting infection. Current conventional antibody-based TB sensing techniques which are commonly used include enzyme-linked immunosorbent assay (ELISA) and interferon-gamma release assays (IGRAs). However, these methods have major drawbacks, such as being time-consuming, low sensitivity, and inability to distinguish between the different stages of the TB disease. Several electrochemical biosensor systems have been reported for the detection of interferon-gamma with high sensitivity and selectivity. Microfluidic techniques coupled with multiplex analysis in regular format and as lab-on-chip platforms have also been reported for the detection of IFN-γ. This article is a review of the techniques for detection of interferon-gamma as a TB disease biomarker. The objective is to provide a concise assessment of the available IFN-γ detection techniques (including conventional assays, biosensors, microfluidics, and multiplex analysis) and their ability to distinguish the different stages of the TB disease.

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A novel label-free electrochemical impedance aptasensor for highly sensitive detection of human interferon-gamma based on target-induced exonuclease inhibition

Cited by 31 publications

References 36 publications

Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Impact of Self-Assembled Monolayer Design and Electrochemical Factors on Impedance-Based Biosensing

Recent Advances in Aptasensor for Cytokine Detection: A Review

Recent advances in the detection of interferon-gamma as a TB biomarker

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