A novel melatonin‐regulated lncRNA suppresses TPA‐induced oral cancer cell motility through replenishing PRUNE2 expression

Su, Shih‐Chi; Yeh, Chia‐Ming; Lin, Chiao‐Wen; Hsieh, Yi‐Hsien; Chuang, Chun‐Yi; Tang, Chih-Hsin; Lee, Yi-Chan

doi:10.1111/jpi.12760

Cited by 58 publications

(40 citation statements)

References 67 publications

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“…However, the association between PRUNE2 expression and its prognostic role in CRC requires further study. Increased methylation of PRUNE2 promoter is associated with nodal metastasis and inversely associated with PRUNE2 expression in head and neck cancer ( 25 ). Further studies are required to identify potential genes that regulate expression of PRUNE2 by regulating the promoter or methylation of PRUNE2 and thus influence the development of CRC.…”

Section: Discussionmentioning

confidence: 99%

PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo

Huang

Yan

et al. 2021

Exp Ther Med

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Prune homolog 2 with BCH domain (PRUNE2) is associated with prostate cancer, neuroblastoma, glioblastoma and melanoma; however, the function of PRUNE2 in colorectal cancer (CRC) remains unknown. The present study aimed to evaluate the effects of PRUNE2 on the development of CRC. The biological function of PRUNE2 in CRC cell lines was investigated by using Cell Counting Kit-8, colony formation, flow cytometry and Transwell assay. Additionally, a mouse model was established to investigate the effect of PRUNE2 on metastasis of CRC cells. The expression levels of PRUNE2 were lower in CRC compared with adjacent normal tissue and this expression pattern was associated with poor relapse-free survival probability. PRUNE2 overexpression significantly decreased cell proliferation and invasion, increased cell apoptosis and arrested the cell cycle. Consistently, it increased the protein expression levels of pro-apoptosis genes and decreased the expression of antiapoptotic proteins. PRUNE2 knockdown had the opposite effects. Furthermore, PRUNE2 overexpression decreased the tumorigenicity of CRC cells. In conclusion, PRUNE2 decreased cell survival, proliferation, invasion and tumorigenicity and promoted apoptosis, suggesting that PRUNE2 may function as a tumor-suppressive gene in CRC.

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Section: Discussionmentioning

confidence: 99%

PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo

Huang

Yan

et al. 2021

Exp Ther Med

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“…For the migration assay: Treated cells in 0.5% FBS medium were loaded into the well of the chamber at the upper part and incubated at 37 °C in 5% CO2 for 24 h. For invasion: 10 μL Matrigel (BD Biosciences, MA, USA) was applied to 8 μm pore size polycarbonate membrane filters that included 10% FBS DMEM medium and then air-dried for 3~4 h in an incubator. The migrated and invaded cells were fixed by methanol for 10 min, stained with Giemsa and counted by light microscopy [20].…”

Section: Migration and Invasion Assaymentioning

confidence: 99%

Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway

et al. 2022

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Arctiin, a lignan glycoside, is isolated from Arctium lappa L. The anticancer effects of arctiin have been demonstrated in several studies. However, no research has been conducted on the anti-migration effect of arctiin in cervical cancer cells. The present study examined the effects of arctiin on cervical cancer cells and investigated the possible molecular mechanism. We demonstrated that arctiin exhibited low cytotoxicity and significantly inhibited cell migration and invasion in human cervical cancer cells. The S100A4 protein expression and mRNA levels were significantly reduced in HeLa and SiHa cells with arctiin treatment. Furthermore, silencing S100A4 by using small interfering RNA reduced cell migration, while overexpression of S100A4 mitigated the migration inhibition imposed by arctiin in cervical cancer cells. Western blotting revealed that arctiin significantly reduced phosphoinositide 3-kinase (PI3K) and phosphorylation of Akt in cervical cancer cells. Moreover, selective Akt induction by an Akt activator, SC-79, reverted cervical cancer cell migration and S100A4 protein expression, which were reduced in response to arctiin. Taken together, these results suggest that arctiin inhibits cervical cancer cell migration and invasion through suppression of S100A4 and the PI3K/Akt pathway.

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“…Melatonin also downregulates melatonin-regulated oral cancer stimulator (MROS-1), suppressing oral migration, refilling the protein homolog 2 (PRUNE). Cell migration induced by MROS-1, modulating PRUNE2, is mediated by an epigenetic mechanism regulated by DNMT3A [ 88 ]. Surprisingly, a recent article reported that high doses of MT could reverse its protective function toward a pro-oncogenic effect.…”

Section: Melatonin a Pleiotropic Moleculementioning

confidence: 99%

Usefulness of Melatonin and Other Compounds as Antioxidants and Epidrugs in the Treatment of Head and Neck Cancer

Guerra¹,

Devesa²

2021

Antioxidants

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Along with genetic mutations, aberrant epigenetic alterations are the initiators of head and neck cancer carcinogenesis. Currently, several drugs are being developed to correct these epigenetic alterations, known as epidrugs. Some compounds with an antioxidant effect have been shown to be effective in preventing these malignant lesions and in minimizing the complications derived from cytotoxic treatment. Furthermore, in vitro and in vivo studies show a promising role in the treatment of head and neck squamous cell carcinoma (HNSCC). This is the case of supplements with DNA methylation inhibitory function (DNMTi), such as epigallocatechin gallate, sulforaphane, and folic acid; histone deacetylase inhibitors (HDACi), such as sodium butyrate and melatonin or histone acetyltransferase inhibitors (HATi), such as curcumin. The objective of this review is to describe the role of some antioxidants and their epigenetic mechanism of action, with special emphasis on melatonin and butyric acid given their organic production, in the prevention and treatment of HNSCC.

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A novel melatonin‐regulated lncRNA suppresses TPA‐induced oral cancer cell motility through replenishing PRUNE2 expression

Cited by 58 publications

References 67 publications

PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo

PRUNE2 inhibits progression of colorectal cancer in vitro and in vivo

Arctiin Inhibits Cervical Cancer Cell Migration and Invasion through Suppression of S100A4 Expression via PI3K/Akt Pathway

Usefulness of Melatonin and Other Compounds as Antioxidants and Epidrugs in the Treatment of Head and Neck Cancer

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