A novel membrane reactor design for controlled studies of interacting populations (simulation of the interaction between microorganism and plant suspension cultures)

Pestchanker, Luis J.; Ercoli, E.

doi:10.1002/(sici)1097-0290(19970820)55:4<609::aid-bit3>3.0.co;2-l

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…In typical mixed-culture assays, the routine procedures used for tracking each strain are time-consuming, difficult and expensive [18]. For this reason, in order to monitor the behavior of each yeast during a multistarter experiment, some methods were based on cell separation by means of a porous membrane, while others were based on the cell immobilization technique [19,20]. …”

Section: Introductionmentioning

confidence: 99%

Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation

et al. 2012

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BackgroundThe use of a multistarter fermentation process with Saccharomyces cerevisiae and non-Saccharomyces wine yeasts has been proposed to simulate natural must fermentation and to confer greater complexity and specificity to wine. In this context, the combined use of S. cerevisiae and immobilized Starmerella bombicola cells (formerly Candida stellata) was assayed to enhance glycerol concentration, reduce ethanol content and to improve the analytical composition of wine. In order to investigate yeast metabolic interaction during controlled mixed fermentation and to evaluate the influence of S. bombicola on S. cerevisiae, the gene expression and enzymatic activity of two key enzymes of the alcoholic fermentation pathway such as pyruvate decarboxylase (Pdc1) and alcohol dehydrogenase (Adh1) were studied.ResultsThe presence of S. bombicola immobilized cells in a mixed fermentation trial confirmed an increase in fermentation rate, a combined consumption of glucose and fructose, an increase in glycerol and a reduction in the production of ethanol as well as a modification in the fermentation of by products. The alcoholic fermentation of S. cerevisiae was also influenced by S. bombicola immobilized cells. Indeed, Pdc1 activity in mixed fermentation was lower than that exhibited in pure culture while Adh1 activity showed an opposite behavior. The expression of both PDC1 and ADH1 genes was highly induced at the initial phase of fermentation. The expression level of PDC1 at the end of fermentation was much higher in pure culture while ADH1 level was similar in both pure and mixed fermentations.ConclusionIn mixed fermentation, S. bombicola immobilized cells greatly affected the fermentation behavior of S. cerevisiae and the analytical composition of wine. The influence of S. bombicola on S. cerevisiae was not limited to a simple additive contribution. Indeed, its presence caused metabolic modifications during S. cerevisiae fermentation causing variation in the gene expression and enzymatic activity of alcohol deydrogenase and pyruvate decarboxilase.

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Section: Introductionmentioning

confidence: 99%

Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation

et al. 2012

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“…Markl et al (1993)). The use and advantages of such a system for the investigation of a defined co-culture have been described ( Pestchanker and Ercoli (1997)), but to our best knowledge not realized up to now.…”

Section: Introductionmentioning

confidence: 99%

Co-cultivation of Lactobacillus zeae and Veillonella criceti for the production of propionic acid

2013

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In this work a defined co-culture of the lactic acid bacterium Lactobacillus zeae and the propionate producer Veillonella criceti has been studied in continuous stirred tank reactor (CSTR) and in a dialysis membrane reactor. It is the first time that this reactor type is used for a defined co-culture fermentation. This reactor allows high mixing rates and working with high cell densities, making it ideal for co-culture investigations. In CSTR experiments the co-culture showed over a broad concentration range an almost linear correlation in consumption and production rates to the supply with complex nutrients. In CSTR and dialysis cultures a strong growth stimulation of L. zeae by V. criceti was shown. In dialysis cultures very high propionate production rates (0.61 g L-1h-1) with final titers up to 28 g L-1 have been realized. This reactor allows an individual, intracellular investigation of the co-culture partners by omic-technologies to provide a better understanding of microbial communities.

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“…Mass transfer by diffusion resulting in a slow homogenization of the fluids between the chambers appears to be a major limitation to this approach. Petchanker and Ercoli [8] discussed the possibility of building a membrane reactor where the exchanges between the culture chambers were achieved by diffusion and convection (filtration) of the medium. However, their approach was theoretical and they only presented a mathematical model and a computer simulation of a microbial interaction.…”

Section: Introductionmentioning

confidence: 99%

Filtration characteristics of hollow fiber microfiltration membranes used in a specific double membrane bioreactor

Günther

Albasi

Lafforgue

2009

Chemical Engineering and Processing: Process Intensification

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A novel membrane reactor design for controlled studies of interacting populations (simulation of the interaction between microorganism and plant suspension cultures)

Cited by 4 publications

References 18 publications

Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation

Starmerella bombicola influences the metabolism of Saccharomyces cerevisiae at pyruvate decarboxylase and alcohol dehydrogenase level during mixed wine fermentation

Co-cultivation of Lactobacillus zeae and Veillonella criceti for the production of propionic acid

Filtration characteristics of hollow fiber microfiltration membranes used in a specific double membrane bioreactor

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