A novel microfluidic RNA chip for direct, single-nucleotide specific, rapid and partially-degraded RNA detection

Zhang, Shun; Chen, Jiuyi; Liu, Dan; Hu, Bei; Luo, Guangcheng; Huang, Zhen

doi:10.1016/j.talanta.2021.122974

Cited by 4 publications

(2 citation statements)

References 45 publications

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“…Combined with reverse transcription recombinase-assisted amplification (RT-RAA), it overcomes the limitations of the NoV detection technology and effectively reduces time, cost and dependence on instruments. In contrast to methods using reverse transcription, Zhang et al [66] reported a new microfluidic RNA microarray (MIRC, a prototype of microchips) strategy based on the genomic replication of DNA polymerase-extended RNA primers on DNA templates with dNTP [67,68], which allowed the direct detection of RNAs without the need for reverse transcription, thus overcoming the tedious reverse transcription process. The method is characterized by rapid detection (within 20 min), high sensitivity, automation and high throughput [69,70].…”

Section: Microchip-based Rna Biosensorsmentioning

confidence: 99%

PCR Independent Strategy-Based Biosensors for RNA Detection

Li,

Wang,

et al. 2024

Biosensors

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RNA is an important information and functional molecule. It can respond to the regulation of life processes and is also a key molecule in gene expression and regulation. Therefore, RNA detection technology has been widely used in many fields, especially in disease diagnosis, medical research, genetic engineering and other fields. However, the current RT-qPCR for RNA detection is complex, costly and requires the support of professional technicians, resulting in it not having great potential for rapid application in the field. PCR-free techniques are the most attractive alternative. They are a low-cost, simple operation method and do not require the support of large instruments, providing a new concept for the development of new RNA detection methods. This article reviews current PCR-free methods, overviews reported RNA biosensors based on electrochemistry, SPR, microfluidics, nanomaterials and CRISPR, and discusses their challenges and future research prospects in RNA detection.

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Section: Microchip-based Rna Biosensorsmentioning

confidence: 99%

PCR Independent Strategy-Based Biosensors for RNA Detection

Li,

Wang,

et al. 2024

Biosensors

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“…Since many life-threatening infectious diseases have similar signs and symptoms, such as cold or u-like syndromes, accurate detection of the pathogens is therefore essential to provide correct treatment regimes. RNA has been recognized as a major biomarker of living pathogens, especially life-threatening RNA viruses like severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and inuenza virus, 1,2 and thus reliable and accurate RNA detection methods are greatly required for pathogen identication and epidemic control. 3 Nucleic acid amplication tests (NAATs) are the most used RNA detection approaches at present due to their excellent sensitivity and high efficiency, among which quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most well known and has been considered the gold standard for SARS-CoV-2 detection.…”

Section: Introductionmentioning

confidence: 99%

Real-time detection of SARS-CoV-2 in clinical samples via ultrafast ligation-dependent RNA transcription amplification

Zhang

et al. 2023

Anal. Methods

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Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions

Tarim

Inevi

Ozkan

et al. 2023

Biomed Microdevices

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The COVID-19 pandemic has posed significant challenges to existing healthcare systems around the world. The urgent need for the development of diagnostic and therapeutic strategies for COVID-19 has boomed the demand for new technologies that can improve current healthcare approaches, moving towards more advanced, digitalized, personalized, and patient-oriented systems. Microfluidic-based technologies involve the miniaturization of large-scale devices and laboratory-based procedures, enabling complex chemical and biological operations that are conventionally performed at the macro-scale to be carried out on the microscale or less. The advantages microfluidic systems offer such as rapid, low-cost, accurate, and on-site solutions make these tools extremely useful and effective in the fight against COVID-19. In particular, microfluidic-assisted systems are of great interest in different COVID-19-related domains, varying from direct and indirect detection of COVID-19 infections to drug and vaccine discovery and their targeted delivery. Here, we review recent advances in the use of microfluidic platforms to diagnose, treat or prevent COVID-19. We start by summarizing recent microfluidic-based diagnostic solutions applicable to COVID-19. We then highlight the key roles microfluidics play in developing COVID-19 vaccines and testing how vaccine candidates perform, with a focus on RNA-delivery technologies and nano-carriers. Next, microfluidic-based efforts devoted to assessing the efficacy of potential COVID-19 drugs, either repurposed or new, and their targeted delivery to infected sites are summarized. We conclude by providing future perspectives and research directions that are critical to effectively prevent or respond to future pandemics.

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A novel microfluidic RNA chip for direct, single-nucleotide specific, rapid and partially-degraded RNA detection

Cited by 4 publications

References 45 publications

PCR Independent Strategy-Based Biosensors for RNA Detection

PCR Independent Strategy-Based Biosensors for RNA Detection

Real-time detection of SARS-CoV-2 in clinical samples via ultrafast ligation-dependent RNA transcription amplification

Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions

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