A novel Miscanthus NAC transcription factor MlNAC10 enhances drought and salinity tolerance in transgenic Arabidopsis

He, Kang; Xiu-ling, Zhao; Chi, Xiaoyuan; Wang, Yiping; Jia, Chunlin; Zhang, Hongpeng; Zhou, Gongke; Hu, Ruibo

doi:10.1016/j.jplph.2019.01.001

Cited by 28 publications

(13 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Agronomical engineering depends on the use of foreign genes, such as the pest resistance cassettes used in a variety of productive GMO crops. Transgenic approaches using plant and non-plant photosynthetic organisms have proved to be useful in conferring tolerance to drought and high soil salinity (Cabello et al, 2017;Dabi et al, 2019;Fan et al, 2019;He et al, 2019;Huang et al, 2017;Li et al, 2017Li et al, , 2018Mushke et al, 2019;Passricha et al, 2019;Sun et al, 2017;Tang et al, 2019;Udawat et al, 2017;Wu et al, 2017;Xu et al, 2018;Yao et al, 2016). Generally, these studies have focused on overexpression or ablation of plant genes to augment halotolerance (Mushke et al, 2019;Passricha et al, 2019;Sun et al, 2017;Tang et al, 2019;Udawat et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Nelson

Hazzouri

Lauersen

et al. 2021

Cell Host & Microbe

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Nelson

Hazzouri

Lauersen

et al. 2021

Cell Host & Microbe

View full text Add to dashboard Cite

“…Plants usually adapt to the environment and abiotic stress by regulating the level of endogenous hormones, especially through the ABA pathway [51,52]. Under drought stress, ABA content in plants increases and stomatal aperture and transpiration decreases, thus improving the drought tolerance of plants.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, many studies have proven that the function of transcription factors relies on the ABA pathway [17]. For example, in Miscanthus, NAC transcription factor MlNAC10 overexpression confers ABA hypersensitivity, and in the transgenetic lines, the expression of four ABA-responsive genes (DREB2A, ERD11, RAB18, and RD29B) was significantly up-regulated [51]. In Arabidopsis, NAC transcription factor VNI2 integrates ABA-mediated abiotic stress signals into leaf senescence by regulating the expression of COR and RD genes [53].…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

Liang

Wang

Sun

et al. 2019

Forests

View full text Add to dashboard Cite

Research Highlights: Phenotypic changes and expression profiles, phylogeny, conserved motifs, and expression correlations of NAC (NAM, ATAF1, ATAF2 and CUC2) transcription factors (TFs) in blueberry genome were detected under drought stress, and the expression patterns and functions of 12 NACs were analyzed. Background and Objectives: Blueberry is an important shrub species with a high level of flavonoids in fruit, which are implicated in a broad range of health benefits. However, the molecular mechanism of this shrub species in response to drought stress still remains elusive. NAC TFs widely participate in stress tolerance in many plant species. The characterization and expression profiles of NAC TFs were analyzed on the basis of genome data in blueberry when subjected to drought stress. Materials and Methods: Combined with the analysis of chlorophyll a fluorescence and endogenous phytohormones, the phenotypic changes of blueberry under drought stress were observed. The phylogenetic tree, conserved motifs, differently expressed genes, and expression correlation were determined by means of multiple bioinformatics analysis. The expression profiles of NACs in different organs were examined and compared through RNA-seq and qRT-PCR assay. Results: The chlorophyll a fluorescence parameters φPo, φEo, φRo, and PIabs of leaves were significantly inhibited under drought stress. ABA (abscisic acid) content noticeably increased over the duration of drought, whereas GA3 (gibberellic acid) and IAA (indole acetic acid) content decreased continuously. A total of 158 NACs were identified in blueberry genome and 62 NACs were differently expressed in leaf and root of blueberry under drought stress. Among them, 14 NACs were significantly correlated with the expression of other NAC genes. Conclusions: Our results revealed the phenotypic changes of this shrub under drought stress and linked them with NAC TFs, which are potentially involved in the process of response to drought stress.

show abstract

“…The physiological parameters, including contents of MDA and hydrogen peroxide, and the enzymatic activities of superoxide (SOD), peroxidase (POD), and catalase (CAT) were measured as previously described ( He et al, 2019a ). Three biological replicates were performed.…”

Section: Methodsmentioning

confidence: 99%

NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco

Wang

Guo

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The NAC (NAM, ATAF1/2, and CUC2) family acts as one of the largest families of the transcription factor in the plant kingdom and was revealed to function as the important regulators in various environmental stresses. However, a few studies were reported about the biofunctions of the NAC transcription factor in tobacco. In the current study, we characterized a novel NAC transcription factor encoding the gene NtNAC053 in tobacco, which was significantly up-regulated when exposed to salt and drought treatments. The results of cis-acting elements analysis suggested that the promoter region of NtNAC053 gene possesses a number of stress-responsive elements, and this gene could be induced by exogenous abscisic acid (ABA) treatment. Moreover, the NtNAC053–GFP fusion protein was localized in the cell nucleus and possessed a transactivation domain in its C-terminal, implying that NtNAC053 may undertake as a transcriptional activator in tobacco. Notably, the overexpression of NtNAC053 in tobacco resulted in hypersensitivity to ABA treatment. Furthermore, these overexpression lines showed significantly enhanced tolerances to drought and salt stresses. Under salt and drought stresses, these overexpression lines possessed higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. Interestingly, the expressions of putative stress-related genes, including NtCOR15A, NtRAB18, NtDREB1A, NtERF5, NtKAT2, and NtERD11, were up-regulated in these overexpression lines when subjected to salt and drought stresses. The clues provided in our study suggested that the NtNAC053 gene encodes a novel NAC transcription factor and could confer the drought and salt stress tolerances by inspiring the downstream stress-responsive genes and antioxidant system in tobacco.

show abstract

A novel Miscanthus NAC transcription factor MlNAC10 enhances drought and salinity tolerance in transgenic Arabidopsis

Cited by 28 publications

References 48 publications

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Identification and Expression of NAC Transcription Factors of Vaccinium corymbosum L. in Response to Drought Stress

NtNAC053, A Novel NAC Transcription Factor, Confers Drought and Salt Tolerances in Tobacco

Contact Info

Product

Resources

About