A novel model of a metastatic human breast tumour xenograft line

Hurst, Josephine M.; Maniar, Nisha B.; Tombarkiewicz, J; Lucas, F Anthony San; Roberson, C.; Steplewski, Z; James, William; Perras, James P.

doi:10.1038/bjc.1993.327

Cited by 39 publications

(29 citation statements)

References 3 publications

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“…The GI101A cell line was provided by the Goodwin Institute for Cancer Research, Inc. (Plantation, FL; ref. 13). The cells were maintained in monolayer culture in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS), L-glutamine, and 5% v/v insulin-selenium-transferrin supplement (Sigma Chemical Co., St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Using a Xenograft Model of Human Breast Cancer Metastasis to Find Genes Associated with Clinically Aggressive Disease

et al. 2005

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Metastasis is the primary cause of death from breast cancer. A xenograft model was used to identify genes potentially involved with metastasis, comparing expression in the poorly metastatic GI101A human breast cancer cell line and a highly metastatic variant, GILM2. cDNA microarray analyses of these isogenic variants were done using 16K Operon 70-mer oligonucleotide microarray slides. Differentially expressed genes were identified by ANOVA, and differences of z2.5-fold were found for 106 genes. Changes in protein or RNA expression were confirmed for 10 of 12 genes. Three markers, heat shock protein 70 (HSP-70), chemokine (C-X-C motif) ligand 1 (CXCL-1), and secreted leukocyte protease inhibitor (SLPI), were studied further with breast cancer tissue microarrays using a novel method of automated quantitative analysis. This uses cytokeratin to define pixels as breast cancer (tumor mask) within the tissue array spot and then measures intensity of marker expression using a cyanine 5-conjugated antibody within the mask. Scores were correlated with clinicopathologic variables. High HSP-70 expression and high nuclear CXCL-1 expression in primary tumors were both associated with decreased survival (P = 0.05 and 0.027, respectively). Expression of each marker was strongly associated with lymph node involvement (P = 0.0002, 0.008, 0.0012, and 0.012 for HSP-70, nuclear CXCL-1, cytoplasmic CXCL-1, and SLPI, respectively). Identification of genes associated with metastasis in experimental models may have clinical implications for the management of breast cancer, because some of these are associated with lymph node metastasis and survival and might be useful as prognostic markers or molecular targets for novel therapies. (Cancer Res 2005; 65(13): 5578-87)

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Section: Methodsmentioning

confidence: 99%

Using a Xenograft Model of Human Breast Cancer Metastasis to Find Genes Associated with Clinically Aggressive Disease

et al. 2005

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“…The breast cancer derived cell lines used in this study were obtained from ATCC, except SK-BR-3 and -7 cells which were from the New York (Sloane Kettering) branch of LICR, CAL-51 (Gionanni et al, 1990) which was obtained from the Dutrillaux Laboratory and GI-101 (Hurst et al, 1993) which was kindly forwarded by Prof CM Steel. The cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM; Gibco/Life Technologies) supplemented with 10% fetal calf serum (FCS).…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

High level expression of differentially localized BAG-1 isoforms in some oestrogen receptor-positive human breast cancers

et al. 1999

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Summary Sensitivity to oestrogens and apoptosis are critical determinants of the development and progression of breast cancer and reflect closely linked pathways in breast epithelial cells. For example, induction of BCL-2 oncoprotein expression by oestrogen contributes to suppression of apoptosis and BCL-2 and oestrogen receptor (ER) are frequently co-expressed in tumours. BAG-1/HAP is a multifunctional protein which complexes with BCL-2 and steroid hormone receptors (including the ER), and can suppress apoptosis and influence steroid hormone-dependent transcription. Therefore, analysis of expression of BAG-1 in human breast cancer is of considerable interest. BAG-1 was readily detected by immunostaining in normal breast epithelial cells and most ER-positive tumours, but was undetectable or weakly expressed in ER-negative tumours. BAG-1 positive cells showed a predominantly cytoplasmic or cytoplasmic plus nuclear distribution of staining. A correlation between ER and BAG-1 was also evident in breast cancer derived cell lines, as all lines examined with functional ER expression also expressed high levels of BAG-1. In addition to the prototypical 36 kDa BAG-1 isoform, breast cancer cells expressed higher molecular weight isoforms and, in contrast to BCL-2, BAG-1 expression was independent of oestrogens. BAG-1 isoforms were differentially localized to the nucleus or cytoplasm and this was also independent of oestrogens. These results demonstrate a close association between BAG-1 and functional ER expression and suggest BAG-1 may be useful as a therapeutic target or prognostic marker in breast cancer.

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“…Furthermore, this model has been refined through the development of 4T1 cell lines to employ varying degrees of metastasis that are location specific and representative of distinct gene expression signatures [150]. Xenograft transplantation encompasses the use of human cancer cells into immuno-compromised mice via intravenous, intraperitoneal or subcutaneous injection, orthptopically or ectopically [136,151]. First discovered in 1962, nude mice have been commonly used for xenotransplantation because they lack a thymus and are unable to mount most types of immune responses, including rejection of allografts and xenografts [152].…”

Section: Tumor Transplantation Modelmentioning

confidence: 99%

Plant Based Natural Products and Breast Cancer: Considering Multi-Faceted Disease Aspects, Past Successes, and Promising Future Interventions

Tobin¹,

Kalupahana²,

Kulka³

2013

Using Old Solutions to New Problems - Natural Drug Discovery in the 21st Century

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A novel model of a metastatic human breast tumour xenograft line

Cited by 39 publications

References 3 publications

Using a Xenograft Model of Human Breast Cancer Metastasis to Find Genes Associated with Clinically Aggressive Disease

Using a Xenograft Model of Human Breast Cancer Metastasis to Find Genes Associated with Clinically Aggressive Disease

High level expression of differentially localized BAG-1 isoforms in some oestrogen receptor-positive human breast cancers

Plant Based Natural Products and Breast Cancer: Considering Multi-Faceted Disease Aspects, Past Successes, and Promising Future Interventions

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