A novel nanoparticle surface-constrained CRISPR-Cas12a 3D DNA walker-like nanomachines for sensitive and stable miRNAs detection

Wang, Xin; Mu, Xiaomei; Jin-shen, LI; Liu, Guang; Zhao, Shulin; Tian, Jianniao

doi:10.1016/j.aca.2023.340950

Cited by 12 publications

(5 citation statements)

References 29 publications

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“…Similarly, Wang et al developed a CRISPR/Cas12a 3D DNA walker-like nanomachine for miRNA detection with high sensitivity and stability. 86 Specifically, the Cas12a activator was confined to spherical nucleic acid (SNA) on the Au nanoparticle surface, performing successive recognition-walking-cleavage reactions. This advanced 3D DNA walker-like nanomachine achieved highly sensitive detection of miRNA-21 with a LOD of 8.0 pM, showing its potential for liquid biopsy applications in disease diagnostics.…”

Section: Enhancing Crispr/cas Liquid Biopsy With Nanoscale Platformsmentioning

confidence: 99%

CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy

Kong,

Yi,

Mintz

et al. 2024

Chem. Commun.

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Section: Enhancing Crispr/cas Liquid Biopsy With Nanoscale Platformsmentioning

confidence: 99%

CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy

Kong,

Yi,

Mintz

et al. 2024

Chem. Commun.

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Section: Recent Biomedical Applications Of Stimuli‐responsive Sna Nan...mentioning

confidence: 99%

“…The LOD for the miRNA detection of this platform was 8 pM. [ 79 ] Specific binding, extension, and cleavage of enzymes to nucleic acid sequences are the core design principles of enzyme‐responsive SNA biosensor. These processes can be converted into amplified output signals so that biological enzymes can be detected with high efficiency.…”

Section: Recent Biomedical Applications Of Stimuli‐responsive Sna Nan...mentioning

confidence: 99%

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Smart Stimuli‐Responsive Spherical Nucleic Acids: Cutting‐Edge Platforms for Biosensing, Bioimaging, and Therapeutics

Tang,

Zhao,

Luo

et al. 2024

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Spherical nucleic acids (SNAs) with exceptional colloidal stability, multiple modularity, and programmability are excellent candidates to address common molecular delivery‐related issues. Based on this, the higher targeting accuracy and enhanced controllability of stimuli‐responsive SNAs render them precise nanoplatforms with inestimable prospects for diverse biomedical applications. Therefore, tailored diagnosis and treatment with stimuli‐responsive SNAs may be a robust strategy to break through the bottlenecks associated with traditional nanocarriers. Various stimuli‐responsive SNAs are engineered through the incorporation of multifunctional modifications to meet biomedical demands with the development of nucleic acid functionalization. This review provides a comprehensive overview of prominent research in this area and recent advancements in the utilization of stimuli‐responsive SNAs in biosensing, bioimaging, and therapeutics. For each aspect, SNA nanoplatforms that exhibit responsive behavior to both internal stimuli (including sequence, enzyme, redox reactions, and pH) and external stimuli (such as light and temperature) are highlighted. This review is expected to offer inspiration and guidance strategies for the rational design and development of stimuli‐responsive SNAs in the field of biomedicine.

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“…[16][17][18] The SNAs of DNA-AuNP conjugates not only possess the unique optical properties of AuNPs and the flexible programmability of oligonucleotides, but also show new properties, such as high level cellular uptake by various cells without the use of transfection agents, increased DNA stability against enzymatic degradation, and low cytotoxicity to cells. These characteristics have made them ideal for nucleic acid detection, [19][20][21][22] biological imaging, 23,24 drug delivery, 25,26 and disease treatment, 27,28 among other applications. 14,29 However, the construction of traditional SNAs is based on the Au-S chemical bond, 30 and it is challenging to precise control the density and orientation of DNA on the surface of AuNPs, thus limiting the performance of target recognition.…”

Section: Introductionmentioning

confidence: 99%