A Novel Poly‐herbal Formulation Hastens Diabetic Wound Healing with Potent Antioxidant Potential: A Comprehensive Pharmacological Investigation

Abstract

Objective: Current investigation was aimed to evaluate the wound healing and antioxidant potentials of the novel Poly-herbal Formulation (PHF) in diabetic rat's models and in-vitro antioxidant assays respectively. Methods: The wound models viz., excision, incision and dead space by using wistar strain albino rats were adopted for this investigation. The free radical scavenging assays were employed for investigation of Antioxidant potential in poly herbal formulation. The constraints studied for assessment of wound repair were percentage closing percentage of wound, tensile strength of repaired tissue, breaking strength of granulation tissue and valuation of hydroxyproline content on dried tissue. The histopathological sections of granulation tissues obtained in dead space wound model were studied for the distribution of collagen and other histopathological changes. Four groups were used in this study with six animals each. Group I and Group II acts as normal control and diabetic control whereas Group III and Group IV was used as glibenclamide treated (1mg/kg b.w.) and PHF (500 mg/kg b.w.) treated group respectively. On the other hand the various antioxidant assays like DPPH, scavenging assays, hydrogen peroxide assay etc. were investigated. Results: The PHF has been significantly healing the wounds in diabetic rats within 18 days, while diabetic control rats healed the wound about 86.12% in the same conditions and time. The wound breaking strength and histopathological studies of healed skin was signified the results. Significant (p<0.01) increase in wound breaking strength as well as epithelialization and the level of hydroxyproline was observed in PHF treated animals comparison with diabetic control group. PHF has also shown significant antioxidant potential in in-vitro assays. Conclusion: All these investigation indicates the significant wound healing and antioxidant potential of PHF in the experimental animal groups in diabetic condition.

Preclinical evidence of polyherbal formulations on wound healing: A systematic review on research trends and perspectives

“… Cassia auriculata, Mangifera indica, Ficus banghalensis, Cinnamomum tamala and Trichosynthis diocia Rats Effective in healing three wound models i.e. excision wound, incision wound and dead space wound models [ 12 ] 7. Plumbago zeylanica Linn, Datura stramonium Linn and Argemone mexicana Linn Polyherbal carbopol- 940 gels Wistar albino rat Antimicrobial, anti-inflammatory accelerated tissue remodeling [ 13 ] 8.…”

“…Data from the earlier studies depicts various experimental wound models such excision model [ 9 , 10 , 12 , 15 , 21 , 26 , 28 ] incision model [ 10 , 12 , 21 ], burn wound model [ 7 , 12 , 20 , 21 ] and dead space wound model [ 10 , 12 ] has been used for evaluating various wound healing potentials of the polyherbal formulations under study. The excision model was prevalent in most of the studies.…”

Combination therapy with Hordeum vulgare, Elettaria cardamomum, and Cicer arietinum exhibited anti-diabetic potential through modulation of oxidative stress and proinflammatory cytokines

“…The underlying reason for using poly-herbal formulations is their superior efficacy and rare side effects than single phytochemical constituents. DM is a complex disorder that can be managed more effectively through the use of PHFs containing multiple bioactive substances [ 36 ]. The majority of polyherbal formulations contain alkaloids, flavonoids, glycosides, polyphenols, tannins, and saponins.…”

Herbal Products and Their Active Constituents for Diabetic Wound Healing—Preclinical and Clinical Studies: A Systematic Review

“…Hydrogels with 4% w/w Blechnum orientale extract exhibited stronger antioxidant activity compared to standards (ascorbic acid, α-tocopherol, BHT as butylohydroksytoluen, and Trolox-C as an analog of vitamin E) and effectively treated diabetic ulcer wounds [ 61 ]. An herbal formulation of Cassia auriculata , Mangifera indica , Ficus banghalensis , Cinnamomum tamala , and Trichosynthis diocia ) [ 32 ], a mixture of alcoholic herbal extracts ( Plantago lanceolata, Arnica montana, Tagetes patula, Symphytum officinale, Calendula officinalis, Geum urbanum ) loaded onto chitosan [ 45 ], ethanol extracts of Salvia kronenburgii and Salvia euphratica ointment [ 48 ], Cotinus coggygria ointments [ 33 ], Quercus infectoria formulations [ 51 ], and kaempferol ointments [ 52 ] showed significant antioxidant activity and improved diabetic wound closure.…”