Objective: Current investigation was aimed to evaluate the wound healing and antioxidant potentials of the novel Poly-herbal Formulation (PHF) in diabetic rat's models and in-vitro antioxidant assays respectively. Methods: The wound models viz., excision, incision and dead space by using wistar strain albino rats were adopted for this investigation. The free radical scavenging assays were employed for investigation of Antioxidant potential in poly herbal formulation. The constraints studied for assessment of wound repair were percentage closing percentage of wound, tensile strength of repaired tissue, breaking strength of granulation tissue and valuation of hydroxyproline content on dried tissue. The histopathological sections of granulation tissues obtained in dead space wound model were studied for the distribution of collagen and other histopathological changes. Four groups were used in this study with six animals each. Group I and Group II acts as normal control and diabetic control whereas Group III and Group IV was used as glibenclamide treated (1mg/kg b.w.) and PHF (500 mg/kg b.w.) treated group respectively. On the other hand the various antioxidant assays like DPPH, scavenging assays, hydrogen peroxide assay etc. were investigated. Results: The PHF has been significantly healing the wounds in diabetic rats within 18 days, while diabetic control rats healed the wound about 86.12% in the same conditions and time. The wound breaking strength and histopathological studies of healed skin was signified the results. Significant (p<0.01) increase in wound breaking strength as well as epithelialization and the level of hydroxyproline was observed in PHF treated animals comparison with diabetic control group. PHF has also shown significant antioxidant potential in in-vitro assays. Conclusion: All these investigation indicates the significant wound healing and antioxidant potential of PHF in the experimental animal groups in diabetic condition.

Purpose:To evaluate the hypoglycaemic activity of the ethanol extract, chloroform fraction, and three compounds isolated from Premna latifolia. Methods: The ethanol extract, chloroform fraction, and compounds 1 -3 (isolated from the chloroform fraction) of P. latifolia were characterised by nuclear magnetic resonance ( 1 H NMR, 13 C NMR and 2D NMR). In vitro activity was assessed by α-amylase and α-glucosidase inhibitory activity assays. In vivo antidiabetic activity was evaluated in a streptozotocin-nicotinamide-induced rat model of diabetes. Fasting blood glucose (FBG), glycosylated haemoglobin (HbA1C), serum creatinine, blood urea nitrogen (BUN), liver enzymes, and antioxidant enzymes were determined. Liver and pancreas histopathology was assessed. Results: Compounds 2 and 3 exhibited significant hypoglycaemic activity by reducing elevated FBG and HbA1C (p ≤ 0.001) and also exerted positive effects on blood and liver enzyme profiles, which were largely altered in diabetic control group. These compounds also showed significant antioxidant activity, increasing catalase, superoxide dismutase, and glutathione reductase, while lowering malondialdehyde (p ≤ 0.001). The histopathology results for the rats that received these compounds suggested regeneration of pancreatic β-cells. Conclusion: Compounds 2 and 3 isolated from the dried roots of P. latifolia possess significant hypoglycaemic activity, reno-and hepatoprotective effects, and antioxidant activity. They show promise as potential treatments for patients with diabetes. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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This investigation aimed to evaluate the wound healing and antioxidant potentials of the novel Poly-herbal Formulation (PHF) in diabetic rat's models and in-vitro antioxidant assays respectively. The wound models viz., excision, incision and dead space by using wistar strain albino rats were adopted for this investigation. The free radical scavenging assays were employed for investigation of Antioxidant potential. The constraints studied for assessment of wound repair were percentage closing percentage of wound, tensile strength of repaired tissue, breaking strength of granulation tissue and valuation of hydroxyproline content on dried tissue. The histopathological sections of granulation tissues obtained in dead space wound model were studied for the distribution of collagen and other histopathological changes. Four groups were used in this study with six animals each. Group I and Group II acts as normal control and diabetic control whereas Group III and Group IV was used as glibenclamide treated (1 mg/kg b.w.), and PHF (500 mg/kg b.w.) treated group respectively. On the other hand the various antioxidant assays like DPPH, scavenging assays, hydrogen peroxide assay etc. were investigated. The PHF has been significantly healing the wounds in diabetic rats within 18 days, while diabetic control rats healed the wound about 86.12 %. Significant (p<0.01) increase in wound breaking strength as well as epithelialization and the level of hydroxyproline was observed in PHF treated animals comparison with diabetic control group. PHF has also shown significant antioxidant potential in in-vitro assays. All these investigations indicate the significant wound healing and antioxidant potential of PHF.

Diabetes mellitus (DM) is chronic disease of endocrine system and one of life threatening problems of the modern era, characterised by high level of glucose in blood. Diabetic patients have the enormous risk of serious infective foot ulcer or wound. The poor wound healing and ineffective functioning with infection in diabetic patient can cause gangrene and finally lead to limb amputation. Herbal medicine, also known as phytomedicine, have been widely used since the ancient times. Studies has been proved that the medicinal plants are rich sources of chemical substances with potential therapeutically and pharmacological activity for treatment of many diseases. Plant shows the presence of various lifesustaining constituents which have potential diabetic wound healing properties. Herbal preparation can be more effective than conventional medicines These phytomedicines are not only cheap and affordable but are also safe.Considering these facts the present review aims to reveal the up to date literature on recent ethnomedical uses with phytochemical review of two different medicinal plants which are Catharanthus roseus, Terminalia chebula commonly used for treatment diabetic wound healing in ayurvedic system of medicine.