Kinase Suppressor of Ras (KSR) is a molecular scaffold that interacts with the core kinase components of the ERK cascade, Raf, MEK, ERK to provide spatial and temporal regulation of Ras-dependent ERK cascade signaling. Interruption of this mechanism can have a high influence in inhibiting the downstream signaling of the mutated tyrosine kinase receptor kinase upon ligand binding. Still none of the studies targeted to prevent the binding of Raf, MEK binding on kinase suppressor of RAS. In that perspective the cysteine rich C1 domain of scaffold proteins kinase suppressor of Ras-1 was targeted rather than its ATP binding site with small ligand molecules like flavones and anthocyanidins and analyzed through insilico docking studies. The binding energy evaluation shows the importance of hydroxyl groups at various positions on the flavone and anthocyanidin nucleus. Over all binding interaction shows these ligands occupied the potential sites of cysteine rich C1 domain of scaffold protein KSR.

Objective: Current investigation was aimed to evaluate the wound healing and antioxidant potentials of the novel Poly-herbal Formulation (PHF) in diabetic rat's models and in-vitro antioxidant assays respectively. Methods: The wound models viz., excision, incision and dead space by using wistar strain albino rats were adopted for this investigation. The free radical scavenging assays were employed for investigation of Antioxidant potential in poly herbal formulation. The constraints studied for assessment of wound repair were percentage closing percentage of wound, tensile strength of repaired tissue, breaking strength of granulation tissue and valuation of hydroxyproline content on dried tissue. The histopathological sections of granulation tissues obtained in dead space wound model were studied for the distribution of collagen and other histopathological changes. Four groups were used in this study with six animals each. Group I and Group II acts as normal control and diabetic control whereas Group III and Group IV was used as glibenclamide treated (1mg/kg b.w.) and PHF (500 mg/kg b.w.) treated group respectively. On the other hand the various antioxidant assays like DPPH, scavenging assays, hydrogen peroxide assay etc. were investigated. Results: The PHF has been significantly healing the wounds in diabetic rats within 18 days, while diabetic control rats healed the wound about 86.12% in the same conditions and time. The wound breaking strength and histopathological studies of healed skin was signified the results. Significant (p<0.01) increase in wound breaking strength as well as epithelialization and the level of hydroxyproline was observed in PHF treated animals comparison with diabetic control group. PHF has also shown significant antioxidant potential in in-vitro assays. Conclusion: All these investigation indicates the significant wound healing and antioxidant potential of PHF in the experimental animal groups in diabetic condition.

No abstract

No abstract

<p class="MsoNormal" style="margin-bottom: .0001pt; text-align: justify; line-height: 150%; mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 12.0pt; line-height: 150%; font-family: 'Times New Roman','serif';">The genus <em>Candida </em>is caused morbidity and mortality in human beings. The virulence factors of the <em>Candida albicans </em>have the great role in the pseudohyphae formation by attached with epithelial cells and endothelial cells. The aim of the study was to evaluate the antifungal activity of weed extracts used in <em>traditional </em>herbal medicine. The weeds were selected on the basis of their reported ethnobotanical uses. Water and alcoholic weed extracts were screened <em>in vitro </em>for their antifungal activity against fungus <em>C. albicans</em>. 50μl concentration of minimal Inhibitory Concentration (MIC) of <em>Mimosa pudica</em> extract in alcohol and 70μl concentration of MIC of </span><em><span style="font-size: 10.0pt; line-height: 150%; font-family: CMTI10; mso-bidi-font-family: CMTI10;">Oxalis corniculata </span></em><span style="font-size: 12.0pt; line-height: 150%; font-family: 'Times New Roman','serif';">extract shows in alcohol. We conclude from this that these extracts exhibit amazing fungicidal properties that support their traditional uses. The presence of phytochemicals in the extracts including, tannins, alkaloids, steroids, glycosides, triterpenoids, flavonoids, phenolic compounds and organic acids like, Malic acid, tartaric acid and citric acid may be responsible for these activities. The acetone extracts of plant are more efficient as compared to the water extract.</span></p><p class="MsoNormal" style="margin-bottom: .0001pt; text-align: center; line-height: 150%; mso-layout-grid-align: none; text-autospace: none;" align="center"> </p>