A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)

Peng, Yu-Chi; Cheng, Chia-Hsuan; Yatsuda, Hiromi; Liu, Szu-Heng; Liu, Shih‐Jen; Kogai, Takashi; Kuo, Chen-Yen; Wang, Chia Hui

doi:10.3390/diagnostics11101838

Cited by 19 publications

(21 citation statements)

References 37 publications

(47 reference statements)

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“…With developments in biosensing technology, SAW has been developed with precise control for promising utility in biosensors. For instance, the shear-horizontal surface acoustic wave (SH-SAW) biosensor is an inventive pathogen detection platform for detecting the anti-SARS-CoV-2 nucleocapsid antibody (IgG-based) [ 118 ]. Although there are still cases of poor sensitivity and an inability to detect small molecules, Yao et al, developed a method for quantifying Escherichia coli L-asparaginase by immobilizing amide-coupled polyclonal antibodies, which significantly improved sensitivity [ 119 , 120 ].…”

Section: Biosensors For the Detection Of Iggmentioning

confidence: 99%

Emerging Methods in Biosensing of Immunoglobin G—A Review

Azam

Bukhari

Liaqat

et al. 2023

Sensors

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Human antibodies are produced due to the activation of immune system components upon exposure to an external agent or antigen. Human antibody G, or immunoglobin G (IgG), accounts for 75% of total serum antibody content. IgG controls several infections by eradicating disease-causing pathogens from the body through complementary interactions with toxins. Additionally, IgG is an important diagnostic tool for certain pathological conditions, such as autoimmune hepatitis, hepatitis B virus (HBV), chickenpox and MMR (measles, mumps, and rubella), and coronavirus-induced disease 19 (COVID-19). As an important biomarker, IgG has sparked interest in conducting research to produce robust, sensitive, selective, and economical biosensors for its detection. To date, researchers have used different strategies and explored various materials from macro- to nanoscale to be used in IgG biosensing. In this review, emerging biosensors for IgG detection have been reviewed along with their detection limits, especially electrochemical biosensors that, when coupled with nanomaterials, can help to achieve the characteristics of a reliable IgG biosensor. Furthermore, this review can assist scientists in developing strategies for future research not only for IgG biosensors but also for the development of other biosensing systems for diverse targets.

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Section: Biosensors For the Detection Of Iggmentioning

confidence: 99%

Emerging Methods in Biosensing of Immunoglobin G—A Review

Azam

Bukhari

Liaqat

et al. 2023

Sensors

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“…Apart from the detection of proteins, Brugger et al [ 114 ] reported the use of SAW for monitoring the formation of neural networks and some investigation for real-time monitoring of living matter, such as organoids or other biomaterials, along with the addition that innovative nanodrugs for efficiency and/or toxicity evaluation can also be imagined [ 115 ]. Since the COVID-19 pandemic began, some SAW-based sensors for COVID virus detection have also been reported [ 116 , 117 ].…”

Section: Current Applicationsmentioning

confidence: 99%

Trends and Applications of Surface and Bulk Acoustic Wave Devices: A Review

2022

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The past few decades have witnessed the ultra-fast development of wireless telecommunication systems, such as mobile communication, global positioning, and data transmission systems. In these applications, radio frequency (RF) acoustic devices, such as bulk acoustic waves (BAW) and surface acoustic waves (SAW) devices, play an important role. As the integration technology of BAW and SAW devices is becoming more mature day by day, their application in the physical and biochemical sensing and actuating fields has also gradually expanded. This has led to a profusion of associated literature, and this article particularly aims to help young professionals and students obtain a comprehensive overview of such acoustic technologies. In this perspective, we report and discuss the key basic principles of SAW and BAW devices and their typical geometries and electrical characterization methodology. Regarding BAW devices, we give particular attention to film bulk acoustic resonators (FBARs), due to their advantages in terms of high frequency operation and integrability. Examples illustrating their application as RF filters, physical sensors and actuators, and biochemical sensors are presented. We then discuss recent promising studies that pave the way for the exploitation of these elastic wave devices for new applications that fit into current challenges, especially in quantum acoustics (single-electron probe/control and coherent coupling between magnons and phonons) or in other fields.

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“…Surface acoustic wave (SAW) biosensors have been used to detect various biomarkers with high selectivity, high sensitivity, and low cost [ 11 , 12 ]. Our previous study showed that shear-horizontal surface acoustic wave (SH-SAW) biosensors can measure anti-SARS-CoV-2 N protein antibodies with good sensitivity [ 13 ]. The SH-SAW biosensor contains an input and output interdigital transducer (IDT), a sensing area coated with antibodies or antigens of the target molecules, and a reflector.…”

Section: Introductionmentioning

confidence: 99%

Measurements of Anti-SARS-CoV-2 Antibody Levels after Vaccination Using a SH-SAW Biosensor

Cheng¹,

Peng

Lin

et al. 2022

Biosensors

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To prevent the COVID-19 pandemic that threatens human health, vaccination has become a useful and necessary tool in the response to the pandemic. The vaccine not only induces antibodies in the body, but may also cause adverse effects such as fatigue, muscle pain, blood clots, and myocarditis, especially in patients with chronic disease. To reduce unnecessary vaccinations, it is becoming increasingly important to monitor the amount of anti-SARS-CoV-2 S protein antibodies prior to vaccination. A novel SH-SAW biosensor, coated with SARS-CoV-2 spike protein, can help quantify the amount of anti-SARS-CoV-2 S protein antibodies with 5 μL of finger blood within 40 s. The LoD of the spike-protein-coated SAW biosensor was determined to be 41.91 BAU/mL, and the cut-off point was determined to be 50 BAU/mL (Youden’s J statistic = 0.94733). By using the SH-SAW biosensor, we found that the total anti-SARS-CoV-2 S protein antibody concentrations spiked 10–14 days after the first vaccination (p = 0.0002) and 7–9 days after the second vaccination (p = 0.0116). Furthermore, mRNA vaccines, such as Moderna or BNT, could achieve higher concentrations of total anti-SARS-CoV-2 S protein antibodies compared with adenovirus vaccine, AZ (p < 0.0001). SH-SAW sensors in vitro diagnostic systems are a simple and powerful technology to investigate the local prevalence of COVID-19.

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A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)

Cited by 19 publications

References 37 publications

Emerging Methods in Biosensing of Immunoglobin G—A Review

Emerging Methods in Biosensing of Immunoglobin G—A Review

Trends and Applications of Surface and Bulk Acoustic Wave Devices: A Review

Measurements of Anti-SARS-CoV-2 Antibody Levels after Vaccination Using a SH-SAW Biosensor

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