A novel sporulation-control gene (spo0M) of Bacillus subtilis with a σH-regulated promoter

Han, Weon-Dong; Kawamoto, Shinichi; Hosoya, Yoshikatsu; Fujita, Masaya; Sadaie, Yoshito; Suzuki, Kenji; Ohashi, Yoshiaki; Kawamura, Fujio; Ochi, Kozo

doi:10.1016/s0378-1119(98)00378-3

Cited by 22 publications

(22 citation statements)

References 29 publications

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“…Moreover, a protein identified as secreted protein (SSQG_01691) was upregulated in MacnA, which is similar to the sporulation control protein Spo0M of B. subtilis [53].…”

Section: Resultsmentioning

confidence: 93%

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Michta

Ding²,

Zhu³

et al. 2014

PLoS ONE

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The aconitase AcnA from the phosphinothricin tripeptide producing strain Streptomyces viridochromogenes Tü494 is a bifunctional protein: under iron-sufficiency conditions AcnA functions as an enzyme of the tricarboxylic acid cycle, whereas under iron depletion it is a regulator of iron metabolism and oxidative stress response. As a member of the family of iron regulatory proteins (IRP), AcnA binds to characteristic iron responsive element (IRE) binding motifs and post-transcriptionally controls the expression of respective target genes. A S. viridochromogenes aconitase mutant (MacnA) has previously been shown to be highly sensitive to oxidative stress. In the present paper, we performed a comparative proteomic approach with the S. viridochromogenes wild-type and the MacnA mutant strain under oxidative stress conditions to identify proteins that are under control of the AcnA-mediated regulation. We identified up to 90 differentially expressed proteins in both strains. In silico analysis of the corresponding gene sequences revealed the presence of IRE motifs on some of the respective target mRNAs. From this proteome study we have in vivo evidences for a direct AcnA-mediated regulation upon oxidative stress.

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“…Moreover, a protein identified as secreted protein (SSQG_01691) was upregulated in MacnA, which is similar to the sporulation control protein Spo0M of B. subtilis [53].…”

Section: Resultsmentioning

confidence: 93%

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Michta

Ding²,

Zhu³

et al. 2014

PLoS ONE

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“…Upstream of BA2305 is the sequence 5Ј-AGGAT ATGACCTATAAAAAAGAAAAACT-3Ј, in which the underlined bases exactly match the consensus for H -dependent promoters (29). B. subtilis spo0M mutants are susceptible to lysis during growth and are impaired in their ability to sporulate (11). Another deleted gene that may affect sporulation is BA2291, a KinA homolog (55.4% similarity, 34.6% identity with the B. subtilis protein).…”

Section: Discussionmentioning

confidence: 99%

Genome Engineering in Bacillus anthracis Using Cre Recombinase

et al. 2006

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Genome engineering is a powerful method for the study of bacterial virulence. With the availability of the complete genomic sequence of Bacillus anthracis, it is now possible to inactivate or delete selected genes of interest. However, many current methods for disrupting or deleting more than one gene require use of multiple antibiotic resistance determinants. In this report we used an approach that temporarily inserts an antibiotic resistance marker into a selected region of the genome and subsequently removes it, leaving the target region (a single gene or a larger genomic segment) permanently mutated. For this purpose, a spectinomycin resistance cassette flanked by bacteriophage P1 loxP sites oriented as direct repeats was inserted within a selected gene. After identification of strains having the spectinomycin cassette inserted by a double-crossover event, a thermo-sensitive plasmid expressing Cre recombinase was introduced at the permissive temperature. Cre recombinase action at the loxP sites excised the spectinomycin marker, leaving a single loxP site within the targeted gene or genomic segment. The Cre-expressing plasmid was then removed by growth at the restrictive temperature. The procedure could then be repeated to mutate additional genes. In this way, we sequentially mutated two pairs of genes: pepM and spo0A, and mcrB and mrr. Furthermore, loxP sites introduced at distant genes could be recombined by Cre recombinase to cause deletion of large intervening regions. In this way, we deleted the capBCAD region of the pXO2 plasmid and the entire 30 kb of chromosomal DNA between the mcrB and mrr genes, and in the latter case we found that the 32 intervening open reading frames were not essential to growth.

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“…Although this gene is not essential for viability of B. subtilis, inactivation of it significantly hinders initiation of endosporulation (17). The absence of this gene in H. modesticaldum is curious since it is present in many species of Bacillus, as well as in sporulating organisms more closely related to heliobacteria, such as D. reducens and P. thermopropionicum.…”

Section: Genome Properties H Modesticaldum Strain Ice1mentioning

confidence: 99%

The Genome of Heliobacterium modesticaldum , a Phototrophic Representative of the Firmicutes Containing the Simplest Photosynthetic Apparatus

et al. 2008

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Despite the fact that heliobacteria are the only phototrophic representatives of the bacterial phylum Firmicutes, genomic analyses of these organisms have yet to be reported. Here we describe the complete sequence and analysis of the genome of Heliobacterium modesticaldum, a thermophilic species belonging to this unique group of phototrophs. The genome is a single 3.1-Mb circular chromosome containing 3,138 open reading frames. As suspected from physiological studies of heliobacteria that have failed to show photoautotrophic growth, genes encoding enzymes for known autotrophic pathways in other phototrophic organisms, including ribulose bisphosphate carboxylase (Calvin cycle), citrate lyase (reverse citric acid cycle), and malyl coenzyme A lyase (3-hydroxypropionate pathway), are not present in the H. modesticaldum genome. Thus, heliobacteria appear to be the only known anaerobic anoxygenic phototrophs that are not capable of autotrophy. Although for some cellular activities, such as nitrogen fixation, there is a full complement of genes in H. modesticaldum, other processes, including carbon metabolism and endosporulation, are more genetically streamlined than they are in most other low-G؉C gram-positive bacteria. Moreover, several genes encoding photosynthetic functions in phototrophic purple bacteria are not present in the heliobacteria. In contrast to the nutritional flexibility of many anoxygenic phototrophs, the complete genome sequence of H. modesticaldum reveals an organism with a notable degree of metabolic specialization and genomic reduction.

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A novel sporulation-control gene (spo0M) of Bacillus subtilis with a σH-regulated promoter

Cited by 22 publications

References 29 publications

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Proteomic Approach to Reveal the Regulatory Function of Aconitase AcnA in Oxidative Stress Response in the Antibiotic Producer Streptomyces viridochromogenes Tü494

Genome Engineering in Bacillus anthracis Using Cre Recombinase

The Genome of Heliobacterium modesticaldum , a Phototrophic Representative of the Firmicutes Containing the Simplest Photosynthetic Apparatus

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