A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

Uma, S.; Karthic, Raju; Kalpana, S.; Backiyarani, S.; Saraswathi, M.

doi:10.1038/s41598-021-99923-4

Cited by 27 publications

(24 citation statements)

References 56 publications

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“…In this research, 10 and 20 mL/explant are shown to be the most suitable condition to increase the number and length of shoots and roots in C. palmata compared to volumes of 5 or 40 mL/explant which recorded the lowest averages. This same trend is reported by Uma et al (2021) in banana (Rasthali AAB-Silk) where a volume of 40 mL/explant of culture medium improves the number and length of shoots compared to 16 and 83 mL that evidence lower multiplication rates and shoot development, using a BIT system; likewise, Arano et al (2020) manage to increase the number and length of shoots with 25 or 50 mL/explant, whereas, a volume of 12.5 and 100 mL causes a decrease in the proliferation rate in C. esculenta. The ndings of this study show that a volume of 20 mL/explant of culture medium (200 mL) is the most suitable condition to increase the number of leaves and dry weight of C. palmata culture.…”

Section: Discussionsupporting

confidence: 90%

“…It is evident that the number of shoots per explant is a function of longer immersion time (5 min). Similar trends are shown byRachmi et al (2019) andAlvarenga and Salazar (2015) who indicate that a prolonged immersion time is critical to increase the rate of shoot proliferation in Aquilaria malaccensis and S. rebaudiana, however, this immersion time is not always optimal to generate plant material of excellent morphological quality, since a prolonged immersion time can cause undesirable physiological disorders such as asphyxia, hyperhydricity and formation of reactive oxygen species, negatively affecting the quality of propagated plant material(Uma et al 2021). The results of this research show that a short immersion time (1 min) is the most suitable condition to increase shoot length, root system development, leaf number and crop dry weight.…”

supporting

confidence: 77%

“…results obtained in carnation (Dianthus caryophyllus L)(Ahmadian et al 2017) and banana (Rasthali AAB-Silk)(Uma et al 2021) where short frequencies increase the number of shoots and roots per explant compared to a prolonged one, however, this condition is not always the most appropriate since they favor tissue hyperhydricity, a physiological disorder that is not observed in this research.…”

mentioning

confidence: 51%

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Micropropagation of iraca palm ( Carludovica palmata Ruiz y Pav)using a temporary immersion system

Minchala

Sánchez

Correa

2022

Preprint

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Carludovica palmata is a neotropical plant with a promising potential for the agroindustry. It is an important source of fibers used to manufacture hand-made goods. Colombia is one of the leading countries in the production this species, however, it lacks efficient techniques for its propagation. We developed a protocol for massive micropropagation of C. palmata using Temporary Immersion Bioreactor (TIB) system. Immersion frequency, immersion times, culture medium volume, and explant density were evaluated using a split-split-plot design. The variables evaluated were number and length of shoots and roots, number of leaves, and explants dry weight. The performance of three micropropagation systems, BIT, semi-solid medium, and liquid medium, were evaluated using generalized randomized block design. Murashige and Skoog (MS) culture medium with 1,0 mg L− 1 of 6-Benzylaminopurine, 0,5 mg L− 1 of 1-Naphthaleneacetic acid, and 20 g L− 1 of sucrose added to it was used. Results obtained was frequency 12 h with immersion time 1 min improved the length of both shoots and roots, as well as the number of leaves and dry weight. Volume of 20 ml/explant was found to be adequate condition to increase the number and length of shoots, the number of leaves, and the dry weight. According to the optimization module, the factors levels that will maximize the evaluated variables are immersion frequency 12 h and immersion time 1 min, with 175 mL of culture medium per bioreactor and density of 10 explants. In conclusion, TIB was shown to be efficient for massive micropropagation of C. palmata compared with conventional methods.

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Section: Discussionsupporting

confidence: 90%

supporting

confidence: 77%

mentioning

confidence: 51%

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Micropropagation of iraca palm ( Carludovica palmata Ruiz y Pav)using a temporary immersion system

Minchala

Sánchez

Correa

2022

Preprint

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“…The use of the highest density of explants that do not cause a diminution on growth increases the efficiency of nutrient uptake and laboratory space. The high inoculation densities can reduce the proliferation of the shoots and increase the contamination (Paek et al, 2001), but in some cases as Curcuma longa and banana the number of plants per bioreactor could be increased in a 50% percentage maintaining optimal growth responses (Marchant et al, 2021;Uma et al, 2021). In Cymbopogon citratus and hazelnut, the low explant density decreased the growth (Quiala et al, 2014;Nicholson et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

A Temporary Immersion System to Improve Cannabis sativa Micropropagation

Rico

Garrido²,

Sánchez

et al. 2022

Front. Plant Sci.

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The aim of this study was to propagate axillary shoots of Cannabis sativa L. using liquid medium in temporary immersion bioreactors. The effect of immersion frequency (3 or 6 immersions per day), explant type (apical or basal sections), explant number (8, 10, and 16 explants), mineral medium (Murashige and Skoog half-strength nitrates, β-A and β-H, all supplemented with 2-μM metatopoline), sucrose supplementation (2, 0.5, and 0% sucrose), culture duration (4 and 6 weeks), and bioreactor type (RITA® and Plantform™) were investigated. As a result, we propose a protocol for the proliferation of cannabis apical segments in RITA® or Plantform™ bioreactors. The explants (8 per RITA® and 24 per Plantform™) are immersed for 1 min, 3 times per day in β-A medium supplemented with 2-μM metatopoline and 0.5% of sucrose and subcultured every 4 weeks. This is the first study using temporary immersion systems in C. sativa production, and our results provide new opportunities for the mass propagation of this species.

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“…4). The physicochemical parameters of the media represented nutrients used for explant metabolism in the TIB (Uma et al, 2021). Sufficient quantities of nutrients must be available for the optimal growth of all explant cells in the TIB.…”

Section: Sugarcane Callus Proliferation In Tib With Different Initial...mentioning

confidence: 99%

Determination of the optimum initial callus weight for the efficient propagation of sugarcane in temporary immersion bioreactor

Saptari¹,

RIYADI²,

SINTA³

et al. 2022

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AbstrakBioreaktor perendaman sesaat (BPS) telah digunakan secara luas untuk propagasi skala massal berbagai tanaman penting, termasuk tanaman tebu. BPS menyediakan sistem kultur semi-otomatis dan kondisi optimal bagi pertumbuhan tanaman. Beberapa faktor menentukan pertumbuhan tanaman pada BPS, salah satunya densitas dari eksplan. Oleh karena itu, penelitian dilakukan untuk menentukan bobot awal yang optimal untuk kalus tebu yang dikulturkan pada BPS, serta mengevaluasi pengaruh perbedaan bobot awal kalus tersebut terhadap proliferasi dan regenerasi kalus tebu. Kalus tebu diinduksi dari daun muda yang masih menggulung dari empat varietas tebu unggul Indonesia. Bobot awal kalus yang dikultur ke dalam bejana TIB yaitu 0,05 g; 0,1 g; 0,2 g; 0,5 g; dan 1,0 g untuk setiap bejana. Kalus kemudian melalui tahap proliferasi pada BPS sebanyak tiga siklus, kemudian kalus diregenerasi pada BPS dengan perlakuan auksin dan sitokinin. Hasil penelitian menunjukkan bahwa 0,2 g merupakan bobot awal kalus yang efisien untuk proliferasi kalus tebu pada TIB, dimana eksponensial multiplikasi kalus tercapai pada bobot awal tersebut, yaitu untuk masing-masing varietas 130,3 kali (PSKA 942), 136,8 kali (PS 094), 21,3 (PS 881), dan 12,9 kali (PS 091) setelah 12 minggu. Densitas kalus pada TIB berkorelasi negatif dengan karakteristik fisikokimia medium. Hal ini menggambarkan variasi intensitas pertumbuhan dan metabolisme kalus dengan adanya perbedaan densitas pada BPS. Penggunaan BAP 0,2 mg L-1 bersama kinetin 0,2 mg L-1 paling sesuai untuk memacu regenerasi kalus tebu dengan menghasilkan jumlah tunas terbanyak dalam waktu relatif lebih cepat (1 – 2 minggu lebih cepat) dibandingkan perlakuan lainnya dan dengan tingkat kejadian pencoklatan yang rendah.[Kata kunci: kultur in vitro, kultur cair, proliferasi]AbstractTemporary immersion bioreactor (TIB) has been utilized for the mass-scale propagation of many important plants, including sugarcane. TIB facilitates a semiautomated culture system and provides optimal conditions for plant growth. Several factors determine plant growth in the TIB, such as explant density. Therefore, an experiment was carried out to determine the optimal initial weight of sugarcane calli and to evaluate its effect on the proliferation and regeneration in TIB. Sugarcane calli were induced from spindle leaves isolated from four Indonesian prime sugarcane varieties. The initial weights of the calli cultured in the TIB flasks were 0.05 g, 0.1 g, 0.2 g, 0.5 g and 1.0 g per flask. The calli were proliferated through three cycles in TIB, and subsequently regenerated in TIB with auxin and cytokinin treatments. The results of the experiments showed that 0.2 g was the most efficient initial weight for sugarcane callus proliferation in the TIB, resulting in an exponential multiplication rate of 130.3-fold (PSKA 942), 136.8-fold (PS 094), 21.3-fold (PS 881), and 12.9-fold (PS 091) within 12 weeks. In the TIB, callus density showed a negative correlation with the physicochemical properties of the medium, demonstrating various growth intensities or metabolic activities of calli at different densities in the TIB. The use of 0.2 mg L-1 BAP along with 0.2 mg L-1 kinetin was suitable for promoting the regeneration of sugarcane calli and producing the highest number of shoots in a relatively short amount of time (1 – 2 weeks faster) with low incidences of browning.[Keywords: in vitro culture, liquid culture, proliferation]

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A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB—Silk)

Cited by 27 publications

References 56 publications

Micropropagation of iraca palm ( Carludovica palmata Ruiz y Pav)using a temporary immersion system

Micropropagation of iraca palm ( Carludovica palmata Ruiz y Pav)using a temporary immersion system

A Temporary Immersion System to Improve Cannabis sativa Micropropagation

Determination of the optimum initial callus weight for the efficient propagation of sugarcane in temporary immersion bioreactor

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