A Paecilomyces fumosoroseus mutant over-producing chitinase displays enhanced virulence against Bemisia tabaci

Hernández-Torres, Ismael; Iracheta, Magdalena; Galán-Wong, Luis J.; Hernández, Carlos Martínez; Contreras, Juan C.; Jackson, Mark A.; Pereyra-Alférez, Benito

doi:10.1023/b:wibi.0000021790.15015.42

Cited by 5 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A stable mutant of I. fumosorosea that over-produces chitinases displayed twofold greater mortality of whitefly than the parental strain (Hernandez-Torres et al 2004). By contrast, a mutant strain of the fungus N. rileyi with decreased chitinolytic activity compared to the parental strain also caused a decreased mortality rate among cabbage looper larvae (El-Sayed et al 1989).…”

Section: Introductionmentioning

confidence: 86%

“…This effect may lead to an increase in virulence (ElSayed et al 1989;Gupta et al 1994). A mutant of the fungus I. fumosorosea that overexpresses chitinases caused a significant increase in mortality of whitefly compared to the parental strain (Hernandez-Torres et al 2004). Conversely, a mutant strain of the fungus Nomuraea rileyi, with decreased chitinase expression, showed a comparative decrease in mortality of cabbage looper larvae (El-Sayed et al 1989).…”

Section: Introductionmentioning

confidence: 96%

“…Combining entomopathogens with enzyme or chemical applications may enhance the pathogenic effect (Bulmer et al 2009) Cuticle-degrading enzymes isolated from three bacteria were studied for their ability to increase effectiveness of Ifr against FST. The enzymes chitinase and lipase have been isolated from entomopathogenic fungi and have been correlated with enhanced virulence (Hegedus and Khachatourians 1988;Mendonsa et al 1996;Hernandez-Torres et al 2004;Nahar et al 2004;Dahiya et al 2006). Selection of an appropriate chitinase for use with fungal entomopathogens is critical because chitinases are thought to be expressed by plants to protect against pathogenic fungi (Taira et al 2002) and are used to control plant pathogens in agricultural applications (Dahiya et al 2006;Karasuda et al 2003).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Improved mortality of the Formosan subterranean termite by fungi, when amended with cuticle-degrading enzymes or eicosanoid biosynthesis inhibitors

Wright

Lax

2015

Folia Microbiol

View full text Add to dashboard Cite

Formosan subterranean termites (FST) were exposed to strains of Beauveria pseudobassiana (Bpb) and Isaria fumosorosea (Ifr) to determine virulence of the fungi. Once lethality was determined, sublethal doses of Bpb were combined with enzymes capable of degrading the insect cuticle to measure the potential to enhance fungal infection. Bpb applied to FST in combination with proteinases and a chitinase caused increased mortality over the fungus alone. Mortality was enhanced when Ifr was applied to FST in combination with a chitinase isolated from Serratia marcesans. A lipase isolated from Pseudomonas cepacia, when combined with Ifr, also resulted in greater mortality than all control treatments. FST were also exposed to the eicosanoid biosynthesis inhibitors (EBIs) dexamethasone (DEX), ibuprofen (IBU), and ibuprofen sodium salt (IBUNA), in combination with Ifr. Combining Ifr with IBUNA caused significantly increased mortality on days 6, 7, and 9. Cuticle-degrading enzymes and EBIs may have potential to enhance the pathogenic effect of a fungal control agent against the Formosan subterranean termite.

show abstract

Section: Introductionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved mortality of the Formosan subterranean termite by fungi, when amended with cuticle-degrading enzymes or eicosanoid biosynthesis inhibitors

Wright

Lax

2015

Folia Microbiol

View full text Add to dashboard Cite

show abstract

“…The insecticidal potential of chitinases has been tested in bioassays with model organisms such as Drosophila melanogaster Meigen. Although there are several examples using pest insects, the studies are based on bacterial, fungal or insect chitinases (Hernández‐Torres et al ., ; Binod et al ., ; Boldo et al ., ; Prasanna et al ., ; Li et al ., ). Research on the effect of plant chitinases on plant‐pest insects is very limited.…”

Section: Introductionmentioning

confidence: 99%

A chitinase from Euphorbia characias latex is a novel and powerful plant‐based pesticide against Drosophila suzukii

Martos

Spanò

Agustí

et al. 2017

Annals of Applied Biology

View full text Add to dashboard Cite

Drosophila suzukii attacks on developing soft fruits have recently caused important economic losses in Europe. This study explores the effectiveness of a new control strategy against this insect pest that is based on a plant chitinase extracted from the latex of the Mediterranean spurge, Euphorbia characias. The ability of the purified Euphorbia latex chitinase (ELC) to degrade the chitin exoskeleton of D. suzukii was assessed using confocal laser scanning microscopy. ELC treatment caused reduced larval growth, higher mortality and notable degradation of external insect structures. Therefore, the chitinase may induce a double effect on the D. suzukii larvae, a direct injury on the larval bodies and an action as antifeedant. The effects of the ELC treatment were also tested on leaves of the insect's host plants, Fragaria × ananassa and Rubus idaeus, using physiological parameters (chlorophyll concentration, chlorophyll fluorescence, leaf gas exchange and water potential) and defence gene expression (FaPGIP, FaChi2_1 and FaChi2_2) as stress indicators. ELC at concentrations effective against D. suzukii did not damage the host plants. Only plant defence gene expression was somewhat enhanced during the early hours after ELC application. In conclusion, ELC, a natural product, proved to be an effective tool for use in the development of an environmentally friendly integrated management strategy against D. suzukii, a pest whose control by conventional chemical insecticides is problematic.A Euphorbia characias chitinase is a powerful plant-based pesticide against Drosophila suzukii

show abstract

Biological Control of Microbial Plant Pathogens in Alternative Sources of Infection

Narayanasamy¹

2013

Biological Management of Diseases of Crops

View full text Add to dashboard Cite

A Paecilomyces fumosoroseus mutant over-producing chitinase displays enhanced virulence against Bemisia tabaci

Cited by 5 publications

References 13 publications

Improved mortality of the Formosan subterranean termite by fungi, when amended with cuticle-degrading enzymes or eicosanoid biosynthesis inhibitors

Improved mortality of the Formosan subterranean termite by fungi, when amended with cuticle-degrading enzymes or eicosanoid biosynthesis inhibitors

A chitinase from Euphorbia characias latex is a novel and powerful plant‐based pesticide against Drosophila suzukii

Biological Control of Microbial Plant Pathogens in Alternative Sources of Infection

Contact Info

Product

Resources

About