A parallel microfluidic flow cytometer for high-content screening

McKenna, Brian; Evans, James G.; Cheung, Monit; Ehrlich, D. J.

doi:10.1038/nmeth.1595

Cited by 99 publications

(108 citation statements)

References 13 publications

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“…Herein we do not intend to describe in details all microfluidic components and applications and we invite the readers to find some interesting lectures in Arora et al, McKenna et al, Cheong et al, Simone et al, and Rillahan et al [49][50][51][52][53][54]. Our intention is to highlight the advantages that microfluidics might have in revealing the glycome code.…”

Section: Microfluidicsmentioning

confidence: 99%

Can Microfluidics boost the Map of Glycome Code?

Simone¹

2014

J Glycomics Lipidomics

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Section: Microfluidicsmentioning

confidence: 99%

Can Microfluidics boost the Map of Glycome Code?

Simone¹

2014

J Glycomics Lipidomics

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“…14,15 Apart from successful application in microdevice for protein analysis, it has been increasingly utilized for constructing cell analyzer. 16,17 However, PDMS is not ideal for cell adhesion owing to an energy barrier between it and cell growth medium. Consequently, tailoring of physical or chemical properties of PDMS to enhance cell adhesion and growth is a key factor for the development of PDMS-based miniaturized cell assay analytical devices.…”

Section: Introductionmentioning

confidence: 99%

Mitigated reactive oxygen species generation leads to an improvement of cell proliferation on poly[glycidyl methacrylate‐co‐poly(ethylene glycol) methacrylate] functionalized polydimethylsiloxane surfaces

Shi

Gao

et al. 2015

J Biomedical Materials Res

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In vitro cell-based analysis is strongly affected by material's surface chemical properties. The cell spreading, migration, and proliferation on a substrate surface are initiated and controlled by successful adhesion, particularly for anchor-dependent cells. Unfortunately, polydimethylsiloxane (PDMS), one of the most used polymeric materials for construction of microfluidic and miniaturized biomedical analytic devices, is not a cell-friendly surface because of its inherent hydrophobic property. Herein, a poly[glycidyl methacrylate-co-poly(ethylene glycol) methacrylate] (poly(GMA-co-pEGMA)) polymer brush was synthesized on a PDMS surface through a surface-initiated atom-transfer radical polymerization method. Contact angle and Fourier transform infrared characterization show that the poly (GMA-co-pEGMA) polymer brush functionalization can increase wettability of PDMS and introduce epoxy, hydroxyl, and ether groups into PDMS surface. In vitro cell growth assay demonstrates that cell adhesion and proliferation on poly(GMA-co-pEGMA) polymer brush-functionalized PDMS (poly(GMA-co-pEGMA)@PDMS) are better than on pristine PDMS. Additionally, immobilization of collagen type I (CI) and fibronectin (FN) on poly(GMA-co-pEGMA)@PDMS is better than direct coating of CI and FN on pristine PDMS to promote cell adhesion. Furthermore, increased intracellular reactive oxygen species and cell mitochondrial membrane depolarization, two indicators of cell oxidative stress, are observed from cells growing on pristine PDMS, but not from those on poly(GMA-co-pEGMA)@PDMS. Collectively, we demonstrate that poly(GMA-co-pEGMA) functionalization can enhance cell adhesion and proliferation on PDMS, and thus can be potentially used for microfluidic cell assay devices for cellular physiology study or drug screening.

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“…40,41 Thus, the possibility to exploit parallel multiple channels working simultaneously with shared inputs has recently been demonstrated. 34,[42][43][44][45] In this scientific context, using the sheath-flow based parallelized system is not possible using a single layer microfluidic device. In this paper, we propose a new method, named self-hydrodynamic focusing, which allows achieving a sheath flow focusing using only one inlet for the sample fluid.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

et al. 2015

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The flow focusing is a fundamental prior step in order to sort, analyze, and detect particles or cells. The standard hydrodynamic approach requires two fluids to be injected into the microfluidic device: one containing the sample and the other one, called the sheath fluid, allows squeezing the sample fluid into a narrow stream. The major drawback of this approach is the high complexity of the layout for microfluidic devices when parallel streams are required. In this work, we present a novel parallelized microfluidic device that enables hydrodynamic focusing in each microchannel using a single feed flow. At each of the parallel channels, a cross-filter region is present that allows removing fluid from the sample fluid. This fluid is used to create local sheath fluids that hydrodynamically pinch the sample fluid. The great advantage of the proposed device is that, since only one inlet is needed, multiple parallel micro-channels can be easily introduced into the design. In the paper, the design method is described and the numerical simulations performed to define the optimal design are summarized. Moreover, the operational functionality of devices tested by using both polystyrene beads and Acute Lymphoid Leukemia cells are shown. V C 2015 AIP Publishing LLC.

show abstract

A parallel microfluidic flow cytometer for high-content screening

Cited by 99 publications

References 13 publications

Can Microfluidics boost the Map of Glycome Code?

Can Microfluidics boost the Map of Glycome Code?

Mitigated reactive oxygen species generation leads to an improvement of cell proliferation on poly[glycidyl methacrylate‐co‐poly(ethylene glycol) methacrylate] functionalized polydimethylsiloxane surfaces

Hydrodynamic self-focusing in a parallel microfluidic device through cross-filtration

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