A peroxidase from bitter gourd (Momordica charantia) with enhanced stability against organic solvent and detergent: A comparison with horseradish peroxidase

Characterization of Plant Peroxidases and Their Potential for Degradation of Dyes: a Review

“…Balady roots was found to show reasonable thermal stability and preserved 87, 51, and 29 % of its maximum activity after 15-min incubation at 50, 60, and 70°C, respectively [50]. Bitter gourd peroxidase retained 50 % of its maximum activity after 60-min incubation at 60°C and showed pH stability in alkaline pH range [8]. Peroxidase from the leaves of J. curcas was reported to show unusual stability under high temperature and extreme pH conditions.…”

“…Peroxidases are present in almost all vascular plants and are assumed to be involved in wide range of physiological phenomena including cross-linking of cell wall polysaccharides [2,3], wound healing, defense mechanisms against pathogens, scavenging of H 2 O 2 from cytosol and chloroplast [4], detoxification of heavy metals and reactive oxygen species during oxidative stress and cellular metabolic processes [5,6], and biodegradation reactions [7]. Plant peroxidases have been exploited for various commercial applications due to their wide substrate specificity and extensive bio-catalytic activities [8]. They have been applied in fluorescent sensors [9], clinical diagnostics [8], and in food manufacturing industry ( [10].…”

“…Plant peroxidases have been exploited for various commercial applications due to their wide substrate specificity and extensive bio-catalytic activities [8]. They have been applied in fluorescent sensors [9], clinical diagnostics [8], and in food manufacturing industry ( [10]. They are also involved in air pollution damage control [11] and bio-bleaching processes [12].…”

“…Moreover, they have potential for soil detoxification, biotransformation of organic compounds, removal of phenolics and aromatic amines from polluted water and water-miscible organic solvents. The abilities of soluble and immobilized peroxidases for decolorization and removal of synthetic dyes from dye-contaminated water and industrial effluents have also been reported [8].…”

Studies on the activity and stability of immobilized horseradish peroxidase on poly(ethylene terephthalate) grafted acrylamide fiber

“…(Activity assay: t = 20°C, Pyrogallol concentration = 10 mmol l -1 ) of the monomers and for obtaining polyphenols of high molecular weight. For synthetic reactions catalyzed by enzymes, stability of the enzymes in the reaction media is important, especially when organic solvents are used [39]. The activity of the free and the immobilized enzymes was determined in the presence of organic solvents (ethanol, methanol, acetone and acetonitrile) whose concentrations in the activity medium 10% (v/v).…”