A pH-sensitive theranostics system based on doxorubicin conjugated with the comb-shaped polymer coating of quantum dots

Abstract

A two-phase route was developed to form a new theranostics-based system. The comb polymer poly (glycidyl methacrylate)-graft-ethane diamine-graft-polyethylene glycol (PGMA-g-EDA-g-PEG) was used to modify the quantum dots (QDs) by the method of ligand exchange. Subsequently, due to a large amount of amino groups on the surface of QDs, the doxorubicin (DOX) was conjugated by amine bonds to form pH-sensitive drug release system. UV-vis transmission spectra and PL spectra showed that the nanoparticles maintained the optical properties of QDs and DOX. The transmission electron microscopy analysis indicated that QDs were well dispersed in water and still had small sizes (7 nm) after ligand exchange and conjugated with DOX. Then the thermogravimetric analysis (TGA) revealed that about 80 wt% comb-shaped polymers coated on the surface of QDs, and about 10 wt% QDs was in nanoparticles PGMAg-EDA-g-PEG-QDs-DOX. In vitro release studies showed that PGMA-g-EDA-g-PEG -DOX and PGMA-g-EDA-g-PEG-QDs-DOX were pH sensitive. Findings from this study suggested that nanoparticles PGMA-g-EDA-g-PEGQDs-DOX can be used in a new field combined both imaging and targeted therapy.

Smart Delivery Systems Based on Poly(glycidyl methacrylate)s‐Coated Organic/Inorganic Core–Shell Nanohybrids

“…Li and Zhao et al coated hydrophilic PGMA‐ b ‐EDA‐ b ‐PEG onto the surface of QDs by ligand exchange method. Importantly, DOX was conjugated via pH‐sensitive imine bonds, developing a pH‐sensitive drug release system ( Figure ) . The hybrids could be stably dispersed in water with small sizes (7 nm), and maintained the optical properties of QDs and DOX.…”

“…Schematic illustration of the synthetic route of QDs@PGEDA‐PEG, DOX loading, and pH‐triggered release. Reproduced with permission . Copyright 2014, Springer.…”

Daunorubicin and gambogic acid coloaded cysteamine-CdTe quantum dots minimizing the multidrug resistance of lymphoma in vitro and in vivo

“…The precipitation was washed three times with deionized water to remove the excess unreacted DNR, and the obtained samples were named as DNR-Cys-CdTe NPs. 25 For the synthesis of DNR-GA-Cys-CdTe NPs, 0.1 mL of DNR⋅HCl (1 mg/mL) and 0.05 mL of Cys-CdTe QDs (2.55×10 -5 M) were added to 0.8 mL of deionized water, and the mixture was stirred for 6 hours. Then, 0.05 mL of GA (1 mg/mL) was added and the mixture was stirred for another 16 hours.…”

Versatile Nanosystem-Based Cancer Theranostics: Design Inspiration and Predetermined Routing

“…The group further reported that 80 wt% of comb-shaped polymers were successfully coated on the surface of QDs, whereas 10 wt% of QDs were incorporated into the nanotheranostic system. UV-Vis transmission and photoluminescence spectra suggested that these nanoparticles maintained the optical properties of QDs and doxorubicin while in vitro release studies showed that PGMA-g-EDA-g-PEG-DOX and PGMA-g-EDA-g-PEG-QDs-DOX were pH sensitive and could be employed in combined imaging and targeted therapy 12 .…”

“…Therefore, multiple versatile systems that have demonstrated superiority both in cancer diagnosis and drug delivery have been created. Nano-based theranostics, which employ quantum dots (QD) 10 - 12 , gadolinium nanoparticles 13 , 14 , carbon nanotubes (CNT) 15 - 17 , gold nanoparticles (AuNP) 18 , 19 , silica nanoparticles (SiNP) 20 , 21 , indocyanine green (ICG) 22 , 23 and chlorin e6 24 , have credibly demonstrated their advantages over traditional diagnostic agents. However, apart from iron oxide nanoparticles which have been approved by U.S. FDA for clinical MRI 25 - 27 , none of the theranostic nanoconstructs has been approved for clinical use.…”