A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells

A Cost-Effective and Non-Invasive pfeRNA-Based Test Differentiates Benign and Suspicious Pulmonary Nodules from Malignant Ones

“…These non-invasive biomarkers belong to a novel type of functional sncRNA. Consistent with our previous reports [17][18][19][20], they showed different lengths in nucleotides (Table 2). Distinct from other integrated prediction models for pulmonary nodules [33-…”

“…These non-invasive biomarkers belong to a novel type of functional sncRNA. Consistent with our previous reports [17][18][19][20], they showed different lengths in nucleotides (Table 2). Distinct from other integrated prediction models for pulmonary nodules [33][34][35][36][37], our prediction rules did not integrate other clinical risk factors, including age [38], smoking history [39][40][41], irregular nodule edges [42], emphysema [43,44], fluorodeoxyglucose-PET avidity [45,46], etc.…”

“…Previously, we showed that pfeRNA is a type of unique functional sncRNAs that plays a critical role in the tumorigenesis and differentiation of non-small cell lung cancer (NSCLC) [17][18][19][20]. Without changing the levels of the target protein, pfeRNA directly binds to its target and regulates the functional behaviors of the target protein [17][18][19][20].…”

“…Previously, we showed that pfeRNA is a type of unique functional sncRNAs that plays a critical role in the tumorigenesis and differentiation of non-small cell lung cancer (NSCLC) [17][18][19][20]. Without changing the levels of the target protein, pfeRNA directly binds to its target and regulates the functional behaviors of the target protein [17][18][19][20]. Since NSCLC and normal human bronchial epithelial cell lines can be distinctly clustered based on the expression patterns of pfeRNAs [17][18][19], we sought to design a blood-based assay based on pfeRNAs.…”

“…Without changing the levels of the target protein, pfeRNA directly binds to its target and regulates the functional behaviors of the target protein [17][18][19][20]. Since NSCLC and normal human bronchial epithelial cell lines can be distinctly clustered based on the expression patterns of pfeRNAs [17][18][19], we sought to design a blood-based assay based on pfeRNAs. We hypothesized that plasma pfeRNAs might be used non-invasively to detect the existence and oncologic nature of an indeterminate pulmonary nodule found on CT scans in patients at high risk for lung cancer.…”