A physiological and molecular study of leukemia patients in Kirkuk city

Abstract

Aim of the study: Determination of liver enzyme [AST(GOT),ALT(GPT),ALK] and evaluate the performance of defferent JAK2 V617F mutation PCR:PCR-ARMS,PCR-RFLP,Real Time-PCR .The patients and methods of work collected samples from the Center for Hematology and Oncology in Kirkuk City , the study included ( 88) patients whose ages ranged between (2-79) and also included ( 40) of natural persons as control cases whose ages ranged between (7-63). In molecular tests, ( 75) a patient sample of the total patient samples was used. The Determination of liver enzyme (AST,ALT,ALK) was performed using several equipment provided by the company (randoxs and Biomerieux) for the studied enzyme, Real-time examination was done using a kit equipped by (Bioneer-Korea) company. Rresults: the results showed the increase in liver enzymes in patients (male and female) compared to (male and female) control at the level of significance. As for the results of molecular tests, the results showed that using the assay (RFLP) that 22 samples gave genotype (GG) and 53 samples gave genotype (GT), as for the assay (ARMS), all samples gave the genotype (GT) Conclusions :High liver enzymes in leukemia patients.

Association of KISS1 Gene SNPs (rs12998) With Endocrine Parameters in Iraqi women Polycystic Ovary Syndrome

“…When comparing cases and controls, it was found that the frequencies of the identified polymorphisms varied significantly. For example, the repetition of the homozygous allele (CC) in cases was greater (15) than in controls (3) while the repetition of the heterozygous allele (CT) did not significantly contrast between cases (56) and controls (61). Women with PCOS had higher allelic frequency of SNP rs12998 C/T with substantial association than control women.…”

“…In order for ovulation to occur and LH to be produced, it is necessary. GnRH and LH secretion are both secreted To a different degree than women with PCOS (15) . the mean serum level of kisspeptin in patient was (1.82±0.43)and in control group was(1.08±0.42) that shows significant difference (≤ 0.0001) in a variety of studies, a rise in serum Kisspeptin levels in PCOS patients was observed, which is consistent with our findings (16,17) .…”