A plasmon-assisted fluoro-immunoassay using gold nanoparticle-decorated carbon nanotubes for monitoring the influenza virus

Abstract

A plasmon-assisted fluoro-immunoassay (PAFI) was developed for the detection of the influenza virus by using Au nanoparticle (Au NP)-decorated carbon nanotubes (AuCNTs) that were synthesized using phytochemical composites at room temperature in deionized water. Specific antibodies (Abs) against the influenza virus were conjugated onto the surface of AuCNTs and cadmium telluride quantum dots (QDs), which had a photoluminescence intensity that varied as a function of virus concentration and a detection limit of 0.1 pg/mL for all three types of influenza viruses examined. The clinically isolated influenza viruses (A/Yokohama/110/2009 (H3N2)) were detected in the range of 50-10,000 PFU/mL, with a detection limit of 50 PFU/mL. From a series of proof-of-concept and clinical experiments, the developed PAFI biosensing system provided robust signal production and enhancement, as well as an excellent selectivity and sensitivity for influenza viruses. This nanoparticle-based technique could be potentially developed as an efficient detection platform for the influenza virus.

Biosensors for waterborne viruses: Detection and removal

“…Lee 10,000 pfu.mL -1 , with a detection limit of 50 pfu.mL -1 . The system provided not only robust signal production and amplification, but also an excellent sensitivity and selectivity for influenza viruses [122]. The application of this work can be extended for the detection of many other viruses.…”

Colorimetric biosensors for point-of-care virus detections

“…QDs nanoparticles are semiconducting materials. They are fluorescent and give off photons of a particular wavelength, and have many applications in virus detection as fluorescent labels [144][145][146][147][148][149]. However, QDs can be modified and used together with other nanostructures to detect viruses by observing for likely color change of a bioassay based on QD-Ru droplet [150].…”

Metal Nanoparticles for Virus Detection

“…[49] Even thoughS ERS-based detection platforms have relativelyi mpressive LOD, they typically require non-conventional, expensive instrumentation and poor target quantification range whichc an limit their applicability.A lso AuNP's plasmon assisted the increaseo ff luorescences ignal of quantum dot to detect influenza virus. [50] AuNP-decorated carbon nanotubes and quantum dots were modified with antibodies. Virus induced the binding between these twor esultingi np lasmonenhanced fluorescencea nd adetection limit of 50 PFU mL À1 .…”