A point-of-care PCR test for HIV-1 detection in resource-limitedsettings

Jangam, Sujit; Agarwal, A.K.; Sur, Kunal; Kelso, David M.

doi:10.1016/j.bios.2012.10.024

Cited by 68 publications

(56 citation statements)

References 24 publications

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“…Another recent portable PCR approach has been developed to integrate microfluidics with a battery-powered quantitative PCR platform. Results using this integrated device have shown a limit of detection of 5 copies/μL, which is superior to the current gold standard RT-qPCR method, with a detection limit of 20 copies/μL [115,116]. In an effort to lower the equipment cost and size for resource-limited settings, it may be beneficial to use super helicases, which are faster, more efficient and can replace the automated heating and cooling steps [117].…”

Section: Recent Advances In Biosensors and Chemical Detection Systmentioning

confidence: 99%

“…Instead, amplification of HIV-1 DNA from peripheral blood mononuclear cells (PBMCs) is used achieve early diagnosis of HIV-1 infected infants. PCR [115], loop-mediated isothermal amplification (LAMP) [135], and recombinase polymerase amplification (RPA) [125] have also been employed to detect HIV-1 DNA. Based on the LAMP technology, a microfluidic biomolecular amplification reader (μBAR) was developed, which integrated molecular biology, microfluidics, optics and electronics.…”

Section: Hiv Management Using Emerging Technologiesmentioning

confidence: 99%

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Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring

Lifson

Özen

İnci

et al. 2016

Advanced Drug Delivery Reviews

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HIV is a major global epidemic that requires sophisticated clinical management. While there have been remarkable efforts to develop new strategies for detecting and treating HIV, it has been challenging to translate them into resource-limited settings. Significant research efforts have been recently devoted to developing point-of-care (POC) diagnostics that can monitor HIV viral load with high sensitivity by leveraging micro- and nano-scale technologies. These POC devices can be applied to monitoring antiretroviral therapy, early infant detection of HIV during mother-to-child transmission, and identification of latent HIV reservoirs. In this review, we discuss current challenges in HIV diagnosis and therapy in resource-limited settings and present emerging technologies that aim to solve these challenges using novel micro- and nanoscale solutions.

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Section: Recent Advances In Biosensors and Chemical Detection Systmentioning

confidence: 99%

Section: Hiv Management Using Emerging Technologiesmentioning

confidence: 99%

Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring

Lifson

Özen

İnci

et al. 2016

Advanced Drug Delivery Reviews

View full text Add to dashboard Cite

show abstract

“…The membrane is then placed directly in a PCR reaction mix for quantitative analysis. Recently, the same group has reported integration of this extraction method with a sample-to-answer PoC PCR system for proviral DNA detection [78]. In this case, blood collected from a heel prick is lysed in the blood collection device, applied to a separator module for FINA, and inserted into an injection-molded assay card.…”

Section: B Global Health Solutionsmentioning

confidence: 99%

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Damhorst

Watkins

Bashir

2013

IEEE Trans. Biomed. Eng.

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“…The nucleic acid based PCR approach extracts HIV-RNA from blood and amplifies RNA detection at 100–200 copies/mL in the case of HIV-1 and HIV-2 category [181]. This PCR system coupled with microfluidic system for precise and accurate RNA extraction from real sample (100μL) can detect DNA at 5,000 copies/mL, which seems suitable for diagnosing HIV at a mature stage [182]. Thus investigating novel tools for rapid selective screening and monitoring of HIV at CNS would be of use for timely diagnostics to optimize cure.…”

Section: Introductionmentioning

confidence: 99%

Getting into the brain: Potential of nanotechnology in the management of NeuroAIDS

Nair

Jayant

Kaushik

et al. 2016

Advanced Drug Delivery Reviews

159

121

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In spite of significant advances in anti-retroviral (ARV) therapy, the elimination of human immunodeficiency virus (HIV) reservoirs from the periphery and the CNS remains a formidable task. The incapability of ARV to go across the blood-brain-barrier (BBB) after systemic administration makes the brain one of the dominant HIV reservoirs. Thus, screening, monitoring, and elimination of HIV reservoirs from the brain remain a clinically daunting and key task. The practice and investigation of nanomedicine possesses potentials for therapeutics against neuroAIDS. This review highlights the advancements in nanoscience and nanotechnology to design and develop specific sized therapeutic cargo for efficient navigation across BBB so as to recognize and eradicate HIV brain reservoirs. Different navigation and drug release strategies, their biocompatibility and efficacy with related challenges and future prospects are also discussed. This review would be an excellent platform to understand nano-enable multidisciplinary research to formulate efficient nanomedicine for the management of neuroAIDS.

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A point-of-care PCR test for HIV-1 detection in resource-limitedsettings

Cited by 68 publications

References 24 publications

Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring

Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring

Micro- and Nanotechnology for HIV/AIDS Diagnostics in Resource-Limited Settings

Getting into the brain: Potential of nanotechnology in the management of NeuroAIDS

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