A practical approach to transferring linear gradient elution methods

High-speed gradient elution reversed-phase liquid chromatography of bases in buffered eluents

“…4); note that the gradient time was simultaneously adjusted to hold the gradient slope constant to avoid changing selectivity [53,54]. We were rather surprised to see that compared to 1 mL/min, the higher flow rates gave better retention time repeatability (< 0.002 min) for the intermediately to highly retained solutes regardless of their retention volume (see Fig.…”

Optimization of the separation of some psychotropic drugs and their respective metabolites by liquid chromatography

“…Both techniques provide reproducible retention times. However, optimization with a gradient should include the dwell volume of the instrument [16]. From the literature, gradients with ternary mixture of solvents are not very popular and chemometric approaches to such gradients have not yet been reported.…”

UPLC–TOF-MS for plant metabolomics: A sequential approach for wound marker analysis in Arabidopsis thaliana

“…5A), two solutions were investigated to obtain highly efficient separation for confirmatory analyses: (1) one single 150 mm column generating about 35,000 plates at a flow rate of 300 L/min and (2) two 150 mm columns coupled in series providing 70,000 plates at a lower flow rate (200 L/min). Because the same stationary phase chemistry was available, a strict geometrical transfer, avoiding changes in selectivity was performed using the basic rules for method transfer [40,45,46]. Gradient profile conditions were obtained taking into account the change in column geometry using a freely usable Excel available on internal website [47].…”