A Predictive Approach Using Fractal Analysis for Analyte–Receptor Binding and Dissociation Kinetics for Surface Plasmon Resonance Biosensor Applications

High sensitivity piezoresistive cantilever design and optimization for analyte-receptor binding

“…k f increases with the vibration frequency of the molecules [11]. Also, Ramakrishnan and Sadana [13] show that the turbulence at the receptor surface, which is produced by varying the surface roughness of the receptor, results in an increase in the effective reaction rate at the surface. They ascribed it to the mixing effect that the turbulence produces, which results in increasing the vibration frequency of the molecules.…”

A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

“…This fractal nature or power-law dependence is exhibited during the association (or binding) and/or the dissociation phases. This fractal power-law dependence has been shown for the binding of antigenantibody (22,32,33), analyte-receptor (27), and analyte-receptorless (protein) systems (34). Very recently, this has been shown to be true for the dissociation phase, too (35,36).…”

“…Apparently, to date, the dissociation kinetics (of the analyte-receptor complex on the surface) has not been discussed or presented in great detail, except in some isolated studies (6,27). This article attempts to address this issue further by analyzing both the association and the dissociation phases of the analyte-receptor kinetics on the biosensor surface.…”

“…These authors noted that the prefactor (in our case, the binding or the dissociation rate coefficient) displays uniform trends with the fractal dimension, D f . Fractal dimension values for the kinetics of antigen-antibody binding (38,39), analytereceptor binding (34), and analyte-receptor binding and dissociation (6,27) are available. We would like to further extend these ideas now to two cases: binding and dissociation phase(s), and the dissociation phase alone as well.…”

SPR: Uma nova ferramenta para biossensores

“…A razão de K diss e K lig (igual a K) pode ser usada para auxiliar a caracterizar o sistema receptor-analito-biosensor/sensor. Os detalhes de associação/dissociação do analito para o receptor, imobilizado sob uma superfície é de grande significado no desenvolvimento de sistemas destinados ao imunodiagnóstico, bem como para sensores e biossensores [93][94][95] . Muitos estudos foram realizados neste âmbito, porém somente a associação e ligação do analito ao receptor é analisada [96][97][98][99][100][101] .…”