A preliminary phylogenetic analysis of ribbed-pine-borer (Rhagium inquisitor) based on mitochondrial COI sequences

Çakmak, Yunus Emre; Soydabaş-Ayoub, Havva Kübra; Uçkan, Fevzi

doi:10.1016/j.aspen.2020.06.008

Cited by 11 publications

(3 citation statements)

References 46 publications

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“…On the contrary, high levels of haplotype diversity were often identified in emerging pest species of longhorn beetles. For example, emerging or population-expanded pest species such as Monochamus sartor, which is one of the famous timber pests [67], Rhagium inquisitor, a ribbed-pine borer [68], and Massicus raddei [69], a serious trunk borer, showed relatively high haplotype diversity of 1.0, 0.708, and 0.96, respectively. Although a high level of genetic diversity generally indicates a large effective population size [70], the high risk of population size decreases or local extinction was reported in this species in known distributions [6,7,14,18].…”

Section: Discussionmentioning

confidence: 99%

Phylogeographic Investigation of an Endangered Longhorn Beetle, Callipogon relictus (Coleoptera: Cerambycidae), in Northeast Asia: Implications for Future Restoration in Korea

Kang

Yi²,

Kuprin

et al. 2021

Insects

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The longhorn beetle, Callipogon (Eoxenus) relictus Semenov, is the only remnant species found in the Palearctic region, while all other Callipogon species are distributed mainly in Central America and partly in South America. This species has been placed in the first category (as ‘critically endangered’) of the Red Data Book in Russia and designated as one of the top-priority target species among all endangered invertebrate species for restoration in South Korea since 2006. Although its restricted distribution in Northeast Asia with a high conservational value has been highlighted, genetic features of C. relictus from different geographic regions remain unexplored. We first investigated the level of genetic diversity and phylogeographic patterns of C. relictus to evaluate the current conservational status and the feasibility of the implementation of a restoration program. The average genetic divergence of mitochondrial gene COI based on Kimura-2-parameter distance among the four regions in Russia, China, North Korea, and South Korea was 2.2%, which lies within the range of intraspecific levels. However, two separate clades with 3.8% divergence were identified, despite no geographical clustering of haplotypes. The linear pattern of the haplotype network with a high level of haplotype and nucleotide diversities suggests that the wide range of currently fragmented populations might be the remnant of genetically diverse populations in the past. This study will provide crucial information on the genetic characteristics and phylogeographic history of C. relictus, which will help to establish conservation strategies for this cherished insect species in Northeast Asia.

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Section: Discussionmentioning

confidence: 99%

Phylogeographic Investigation of an Endangered Longhorn Beetle, Callipogon relictus (Coleoptera: Cerambycidae), in Northeast Asia: Implications for Future Restoration in Korea

Kang

Yi²,

Kuprin

et al. 2021

Insects

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“…The primer pairs HCO2198–LCO1490 (Folmer et al, 1994) and HCO2198-JJ-LCO1490-JJ (Astrin & Stüben, 2008) for the mitochondrial cytochrome c oxidase I ( COI ) gene region, LR-J-12887-LR-N-13398 (Yoon et al, 2001) for mitochondrial 16S rRNA gene region, and LSU D1, D2 fw1: 56-74 and LSU D1, D2r rev2: 1048-1067 (Sonnenberg et al, 2007) for nuclear 28S rRNA gene regions were used in amplification. Mitochondrial COI and 16S RNA gene regions were amplified by using the same thermocycling parameters as 1 min at 95 °C, five cycles of 30 s at 95 °C, 1 min at 46 °C, 1 min at 72 °C, 30 cycles of 30 s at 95°C, 1 min at 51°C, 1 min at 72 and 10 min at 72 °C (Çakmak et al, 2020). The thermocycling parameters used for amplification of the nuclear 28S rRNA gene region were 1 min at 95 °C, four cycles of 30 s at 95 °C, 1 min at 57,3 °C, 1 min at 72 °C, and 20 cycles of 30 s at 94 °C, 1 min at 61,7 °C, 1 min at 72 °C and 10 min at 72 °C (Soydabaş-Ayoub et al, under review).…”

Section: Methodsmentioning

confidence: 99%

Time-scaled phylogenetic analysis of extant Lamiinae (Coleoptera, Cerambycidae) species of East of Marmara Basin, Türkiye, and their evolutionary similarity with the Eurasian congeners

Soydabaş-Ayoub

Uçkan

2022

Preprint

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Lamiinae is a tremendous subfamily of Cerambycidae, with around 20,000 members dispersed across continents. The knowledge of the evolutionary history of the subfamily is scarcely, and there are growing doubts about the phylogenetic relationships due to the recognised illusion caused by the convergence of the morphological characters. The present study contributes to the evolutionary history and phylogenetic relationships of the tribes Acanthocinini, Acanthoderini, Agapanthiini, Batocerini, Dorcadionini, Lamiini, Mesosini, Monochamini, Phytoeciini, Phrynetini, Pogonocherini (including Exocentrini) and Saperdini with Neighbor-Joining (NJ), Maximum Likelihood (ML) and time-scaled Bayesian analyses based on partial mitochondrial COI and 16S rRNA and nuclear 28S rRNA gene regions (2257 base pair alignment length). The most recent common ancestor (MRCA) of the taxa included in the analyses appeared during the Middle or Late Cretaceous, and the MRCAs of the closely related tribes emerged in Paleogene. The MRCA of Dorcadionini, Lamiini and Monochamini was younger than the common ancestors of the other close tribes. The hypothetical ML phylogram was consistent with the Bayesian chronogram in the proximity of Batocerini to Lamiini, Acanthocinini to Acanthoderini, Phyretrini to Pogonocherini, and Phytoeciini to Saperdini, in addition to the affiliation of Lamiini, Dorcadionini and Monochamini. At the COI-based NJ and ML gene trees, Paraleprodera and Lamia (Lamiini) were sisters to Imantocera (Gnomini), Oberea (Obereini) to Phytoecia (Phytoeciini), and Hippopsis (Agapanthiini) to Omosarotes (Acanthomerosternoplini). The present results support Dorcadionini Gnomini and Monochamini as synonyms of Lamiini; and Obereini and Phytoeciini of Saperdini. We suggest that the emergence of the living tribes included in this study was during Paleogene, and the intrageneric diversifications occurred in Cenozoic, mostly during Neogene.

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“…hybridising species, maternally-inherited endosymbionts) (Raupach et al 2020), numerous studies across a broad range of taxa have already proven that DNA barcoding provides a useful tool to speed up taxonomic procedures, such as automatic species identification by matching new sequences to existing taxa in reference libraries (e.g. Schmidt et al 2015;Grebennikov et al 2017;Coral Şahin et al 2019;Kelnarova et al 2019;Schmid-Egger et al 2019;Çakmak et al 2020), associating dimorphic sexes (e.g. Zhai et al 2017;Zhang et al 2019) or different life stages (e.g.…”

Section: Introductionmentioning

confidence: 99%

The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

Karpiński¹,

Szczepański²,

Plewa³

et al. 2021

ASP

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This paper sheds the first light on the phylogeny of the Central Asian genus Turanium Baeckmann, 1922. By applying an integrative taxonomy approach, we revealed and described a new species from Kyrgyzstan—Turanium losi Karpiński, Plewa & Hilszczański sp. nov. Distinguishing characters from closely related Turanium pilosum (Reitter, 1891) are presented and their ecological associations are discussed. The key characters, including the male terminalia, were examined by means of scanning electron microscopy. High-quality stacked photographs of the habitus of the specimens are presented for both species and their geographical distributions are mapped. While the new species shows stable morphological characters that allow its differentiation from T. pilosum and the COI genetic distance between them is approx. 3%, the different species delimitation methods gave discordant results. Although the new species remained unrecognized for so long, it seems that these cerambycids are common in the region and both can be considered potentially invasive as they are apparently highly polyphagous. It has also been documented that they occur sympatrically in Kyrgyzstan. Both the Bayesian and maximum likelihood analyses of COI sequences confirmed the monophyly of the genus Turanium with strong support (PP 1 and BS 90, respectively). Moreover, the recently revealed polyphyly of the tribe Callidiini was supported by our analyses and, consequently, the discussion on the establishment of a new tribe Ropalopini is raised. This study further corroborates the effectiveness of DNA barcoding as a tool in detecting new species and provides some of the first sequences for Central Asian cerambycids, which remain almost completely unknown in terms of molecular studies.

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A preliminary phylogenetic analysis of ribbed-pine-borer (Rhagium inquisitor) based on mitochondrial COI sequences

Cited by 11 publications

References 46 publications

Phylogeographic Investigation of an Endangered Longhorn Beetle, Callipogon relictus (Coleoptera: Cerambycidae), in Northeast Asia: Implications for Future Restoration in Korea

Phylogeographic Investigation of an Endangered Longhorn Beetle, Callipogon relictus (Coleoptera: Cerambycidae), in Northeast Asia: Implications for Future Restoration in Korea

Time-scaled phylogenetic analysis of extant Lamiinae (Coleoptera, Cerambycidae) species of East of Marmara Basin, Türkiye, and their evolutionary similarity with the Eurasian congeners

The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

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