A Preliminary Report on the Neutralization of the Lymphocytosis-Promoting Factor (Lpf) With Anti-LPF Serum

Ishida, Susumu; Asakawa, Sadao; Kurokawa, Masami

doi:10.7883/yoken1952.23.67

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…1B7 may detach more easily from the cell-PT-MAb complex by being washed compared with the effects for 11E6. The results of cell infusion experiments suggest that PT interacts reversibly or successively with target cells in vivo as reported previously (1,3,22) and also suggest that the epitope for 1B7 may not be related to the initial binding site of the cell. As four distinct MAbs were characterized in this study, they can be used to analyze the complex process of PT activity in vivo by an in vitro system.…”

Section: Discussionsupporting

confidence: 80%

Effect of monoclonal antibody to pertussis toxin on toxin activity

Sato¹,

Sato²,

Ito³

et al. 1987

Infect Immun

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Two distinct monoclonal antibodies, one to pertussis toxin subunit S2, called 9G8, and another to subunits S2 and S3, called 11E6, were generated from the hybridomas of myeloma SP2/0 and spleen cells of BALB/c mice immunized mainly with the subunit S234 complex. Binding ability of 9G8 and 11E6 to the subunits was confirmed by the enzyme-linked immunosorbent assay and immunoblotting analysis. Generation of 11E6 bound to both S2 and S3 might mean that there is common antigenicity between S2 and S3. Neutralizing activities of 9G8 and 11E6 on various biological activities of pertussis toxin, including ADP-ribosyltransferase and leukocytosis-promoting, islet-activating, permeability-increasing. Chinese hamster ovary (CHO) cell-clustering, and hemagglutinating activities, were compared with those of anti-S1 monoclonal antibodies 1B7 and 3F10, which were isolated and characterized in a previous study (H. Sato, A. Ito, J. Chiba, and Y. Sato, Infect. Immun. 46:422-428, 1984). 1B7 and 3F10 neutralized ADP-ribosyltransferase activity of pertussis toxin or S1, but 9G8 and 11E6 did not. 1B7 showed very potent neutralization against leukocytosis-promoting, islet-activating, permeability-increasing, and CHO cell-clustering activities of pertussis toxin, but 3F10 did not, although anti-ADP-ribosyltransferase activities of both antibodies were identical. 11E6 neutralized leukocytosis-promoting, islet-activating, CHO cell-clustering, and hemagglutinating activities but not permeability-increasing activity. 9G8 showed slight neutralization of leukocytosis-promoting and CHO cell-clustering activities. Specific activities of 1B7 and 11E6 in each neutralization test were higher than or almost comparable to those of polyclonal antibodies to pertussis toxin. The neutralizing mechanism of 1B7 and 11E6 in leukocytosis-promoting activity was compared. 11E6 seemed to interfere with the binding of pertussis toxin to receptors on mouse spleen cells.

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Section: Discussionsupporting

confidence: 80%

Effect of monoclonal antibody to pertussis toxin on toxin activity

Sato¹,

Sato²,

Ito³

et al. 1987

Infect Immun

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“…Such reversibility was also suggested from a finding that lymphocytosis in mice induced by LPF was cured rapidly with an anti-LPF serum (Ishida, et al, 1970). The most reasonable explanation from the available observations may be that the change of lymphocytes may result from a mere adsorption of the LPF molecules by some constituents on the cell surface which are indispensable for the selective migration through the endothelial cells of the post-capillary vannules in the lymph nodes.…”

Section: Discussionmentioning

confidence: 88%

Effects of the Lymphocytosis-Promoting Factor From Bordetella Pertussis on the Function and Potentiality of Lymphocytes

Iwasa

Yoshikawa

Fukumura

et al. 1970

JJMSB

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Interaction of Lymphoid and Nonlymphoid Cells with the Lymphocytosis-Promoting Factor of Bordetella pertussis

Adler

Morse

1973

Infect Immun

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The activity of soluble lymphocytosis-promoting factor (LPF) from Bordetella pertussis decreased after brief in vitro incubation with lymphoid cells or erythrocytes. The LPF activity was found to be associated with the cells used, and injection of the cells produced a leukocytosis and lymphocytosis which completely accounted for the loss of soluble activity. Attachment of LPF to cells was found to be reversible in vitro. It is suggested that reversible binding occurs in vivo.

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A Preliminary Report on the Neutralization of the Lymphocytosis-Promoting Factor (Lpf) With Anti-LPF Serum

Cited by 4 publications

References 5 publications

Effect of monoclonal antibody to pertussis toxin on toxin activity

Effect of monoclonal antibody to pertussis toxin on toxin activity

Effects of the Lymphocytosis-Promoting Factor From Bordetella Pertussis on the Function and Potentiality of Lymphocytes

Interaction of Lymphoid and Nonlymphoid Cells with the Lymphocytosis-Promoting Factor of Bordetella pertussis

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