A process for polyhydroxyalkanoate (PHA) production from municipal wastewater treatment with biological carbon and nitrogen removal demonstrated at pilot-scale

Bengtsson, Stina; Karlsson, Anton; Alexandersson, Tomas; Quadri, Luca; Hjort, Markus; Johansson, Peter; Morgan-Sagastume, Fernando; Anterrieu, Simon; Arcos-Hernández, Mónica V.; Karabegovic, Lamija; Magnusson, Per; Werker, Alan

doi:10.1016/j.nbt.2016.11.005

Cited by 125 publications

(45 citation statements)

References 42 publications

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“…The excess activated sludge biomass from a biological wastewater treatment process can be harvested and be made to store PHA to more than its dry weight in a separate accumulation process under conditions of prolonged feast [8]. These ideas have been featured in the literature at bench scale [9], but also now increasingly at pilot scales [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Grazia

Quadri

Majone

et al. 2017

Journal of Environmental Chemical Engineering

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Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater, Journal of Environmental Chemical Engineeringhttp://dx.Engineering (Brisbane, Queensland, Australia); 4 Promiko AB (Lomma, Sweden).Corresponding author: Alan Werker (alan@werker.se) Graphical abstractHighlights  Enrichment biomass at 15 and 25 °C gave similar PHA accumulation potential  PHA accumulation process robust to temperatures from 15 to 30°C  Kinetics change with temperature according to modified Arrhenius equation  Mixed culture PHA production is more efficient at lower temperatures  Bioprocess is adaptable to anticipated seasonal water temperature changes AbstractThe influence of temperature on the production of enrichment biomass and polyhydroxyalkanoates by activated sludge was evaluated within a practical case study. Two laboratory-scale sequencing batch reactors (SBRs) were operated in parallel over 131 days to treat a wastewater from a potatostarch modification facility, and produce surplus activated sludge biomass with PHA accumulation potential. The SBRs were operated similarly but at different temperatures (15 and 25°C).Temperature did not influence wastewater treatment performance (average 97% COD removal).Replicate PHA accumulation experiments were conducted on the SBR surplus biomass at 15, 20, 25 and 30°C. Surplus biomass accumulated PHA with acetic acid to between 60 and 65%, gPHA/gVSS for all temperatures tested and with estimated Arrhenius temperature coefficients (θ) of 1.048 for the PHA production, and 1.062 for the COD consumption specific rates. The MMC PHA production process was adaptable within a common range anticipated for seasonal fluctuation of temperature for the influent wastewater and PHA production feedstocks without loss of performance. This outcome suggests a broad range in the practical feasibility and reliability with MMC method and process implementations. PHA production was predicted to be more efficient at lower temperatures. Strategies can be engineered in the bioprocess design and operations to accommodate for temperature shifts in practical applications of PHA production as a route to resource recovery from organic waste management services.

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Section: Introductionmentioning

confidence: 99%

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Grazia

Quadri

Majone

et al. 2017

Journal of Environmental Chemical Engineering

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“…The activated sludge used for the present study is considered to be enriched with the PHA storing phenotype due to a zone of anoxic feast at the head of the biological treatment process. Heterotrophic PHA storing denitrifiers have been enriched in previous fundamental studies both at laboratory and pilot scale [9,10]. Simultaneous nitrification and denitrification (SND) was similarly observed in another study at low DO levels (0.5 mg/L) during the aerobic phase in an enhanced biological phosphorus removal process [31].…”

Section: Accepted Manuscriptmentioning

confidence: 80%

“…integrating PHA production with biological nitrogen (and phosphorus) removal from the process water at a sugar factory [9]. High PHA productivity (up to 49% g-PHA/g-VSS) was also accomplished with biological carbon and nitrogen removal from domestic wastewater at pilot scale with an integrated fixed-film activated sludge process [10].…”

Section: Accepted Manuscript Introductionmentioning

confidence: 99%

Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge

Wang¹,

Bengtsson²,

Oehmen

et al. 2019

New Biotechnology

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“…However, being aerobic, these processes are energy intensive (1 kg PHA production requires ~39 MJ), when the aerobic accumulation step is used [137]. WWT has been using streams with high VFAs resulting from fermentation of solids to produce enhanced biomass along with high PHA accumulation [135,143,144], which has been reported as unnecessary [140]. Therefore, the conversion of highly concentrated VFA-media into only polymers seems to be a good choice rather than biomass and polymers.…”

Section: Bio-plasticsmentioning

confidence: 99%

Opportunities and challenges in sustainable treatment and resource reuse of sewage sludge: A review

Raheem

Sikarwar

et al. 2018

Chemical Engineering Journal

614

165

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A process for polyhydroxyalkanoate (PHA) production from municipal wastewater treatment with biological carbon and nitrogen removal demonstrated at pilot-scale

Cited by 125 publications

References 42 publications

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Influence of temperature on mixed microbial culture polyhydroxyalkanoate production while treating a starch industry wastewater

Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge

Opportunities and challenges in sustainable treatment and resource reuse of sewage sludge: A review

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