A Proposal for Zero Emission from Palm Oil Industry Incorporating the Production of Polyhydroxyalkanoates from Palm Oil Mill Effluent.

Hassan, Mohd Ali; Nawata, Osamu; Shirai, Yoshihito; Rahman, Norizah Abdul; Yee, Phang Lai; Ariff, Arbakariya; Karim, Mohamed Ismail Abdul

doi:10.1252/jcej.35.9

Cited by 36 publications

(12 citation statements)

References 6 publications

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“…Production of organic acids (Hassan et al 1997;Tabassum et al 2008), methane (CH 4 ) (Yacob et al 2006) and biohydrogen (Chong et al 2009) from POME were evidences of the presence of potential carbon and nitrogen sources al well as biological biodiversity in the POME sludge. Production of mixed organic acids from anaerobicallay treated palm oil mill effluent has introduced it as a shown renewable and cheaper carbon sources for PHAs production (Hassan et al 1997(Hassan et al , 2002Zakaria et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate production from anaerobically treated palm oil mill effluent by new bacterial strain Comamonas sp. EB172

Zakaria

Tabatabaei

Ghazali

et al. 2009

World J Microbiol Biotechnol

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A new isolate designated as strain EB172 was isolated from a digester treating palm oil mill effluent and was investigated by polyphasic taxonomic approach. The cells were rod-shaped, Gram-negative, non-pigmented, non-spore-forming and non-fermentative. Phylogenetic analysis using the 16S rRNA gene sequence showed that the strain clustered with the genus Comamonas. Its closest neighbours were the type strains Comamonas terrigena (96.8%), Comamonas koreensis (93.4%), Comamonas composti (92.9%), and Comamonas kerstersii (91.1%). The ability of the strain EB172 to produce polyhydroxyalkanoates (PHA) when supplied with organic acids made this bacterium unique among Comamonas species. The bacterial strain was clearly distinguished from all of the existing strains by phylogenetic analysis, fatty acid composition and a range of physiological and biochemical characteristics. The G?C content of the genomic DNA was 59.1 mol%. The strain showed good growth in acetic, propionic and nbutyric acids. Comamonas sp. EB172 produced 9.8 g/l of cell dry weight and accumulated 59 (wt%) of PHAs when supplemented with mixed organic acids from anaerobically treated palm oil mill effluent. It is evident from the genotypic, phenotypic data and ability to produce PHAs that strain EB172 represents a new strain in the genus Comamonas (GeneBank accession no. EU847238).

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Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate production from anaerobically treated palm oil mill effluent by new bacterial strain Comamonas sp. EB172

Zakaria

Tabatabaei

Ghazali

et al. 2009

World J Microbiol Biotechnol

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“…Recently, waste streams have been sought for use as nutrient sources in production of value added products, such as PHA [57][58][59][60][61] and TAGs [62]. Hassan and coworkers have constructed a method for producing PHA from organic acids resulting from digestion of processing sludge from palm oil mill effluent [60]. Polymer has also been produced from pure cultures using whey [61,63], beet molasses [64], inedible jatropha oil [42], and waste glycerol [58].…”

Section: Pha Production Challengesmentioning

confidence: 99%

Bacterial Carbon Storage to Value Added Products

Brigham¹,

Kurosawa²

2011

JMBT

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Microorganisms have evolved different systems for storing carbon during times of stress. In the cell's natural environment, the stored carbon can then be utilized for growth when other nutrients are in better supply. Storage of carbon and other nutrients is ubiquitous throughout the prokaryotic and eukaryotic domains of life. These carbon storage molecules have great industrial importance. They can be useful as value-added products, as either biopolymers or biofuels, and cells are grown in large quantities and these compounds are harvested, usually as a replacement for a petroleum-based product. Nowadays, entire industries have been generated based on the production and utilization of these compounds. We focus on two bacteria that could be considered paradigms of their particular carbon storage strategy: Ralstonia eutropha and Rhodococcus opacus. R. eutropha has been well-studied as a polyhydroxyalkanoate (bioplastic) producer and R. opacus is a model bacterium for high yield triacylglycerol (TAG) production for biofuels. Both species produce carbon storage molecules that can potentially diminish our reliance on fossil-based petroleum. However, in both cases, there are challenges that must be overcome before profitable production schemes are established using these organisms. We explore the previous and current works to address these challenges in this review.

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“…Although the proposed overall zero emission system appeared to be practical, major drawbacks were found, including the rather low yield and productivity of PHA by R. eutropha when the concentrated organic acids from PO ME were used as compared to synthetic organic acids. This could be due to the high presence of ammonium (1.5 g/L) or other compounds in the anaerobically digested POME concentrate (Hassan et al, 2002). Md Din et al (2006b) proposed the suitability of using mixed cultures to produce PHA in POME since most prokaryotes are capable of PHA production.…”

Section: Biopolymer Production Using Pomementioning

confidence: 99%