A proteomic analysis of the wound response in Medicago leaves reveals the early activation of a ROS-sensitive signal pathway

Soares, Nelson C.; Wojtkowska, J.; Jackson, P

doi:10.1016/j.jprot.2011.03.017

Cited by 21 publications

(17 citation statements)

References 43 publications

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“…Both H 2 O 2 and LOOH were reported to increase in the plant tissues subjected to mechanical stress (Fabbri et al 2000; Orozco-Cardenas et al 2001; Soares et al 2011). In the present study, LOOH levels in leaves subjected to short-term salt treatment increased sharply during the initial hours after the onset of incubation (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression Analysis of a Gene Encoding for Ascorbate Peroxidase and Responsive to Salt Stress in Beet (Beta vulgaris)

et al. 2013

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BvpAPX is a full-length cDNA-encoding peroxisomal ascorbate peroxidase isolated from leaves of salt-stressed beet (Beta vulgaris) plants. A high level of identity has been reported between the deduced amino acid sequence of BvpAPX and other known ascorbate peroxidases. The genomic sequence of BvpAPX revealed a gene composed of 5 exons and 4 introns. Several sequence motifs revealed in the 5’UTR region of the gene confer to BvpAPX a putative responsiveness to various abiotic stresses. We determined the effect of salt stress on BvpAPX expression in leaves of the cultivated beet varieties, Huzar and Janosik, and their wild salt-tolerant relative B. vulgaris ssp. maritima. Plants were subjected to salt stress during a 32-day culture period (long-term salt treatment). An alternative salinization protocol consisted of an 18-h incubation of detached beet leaves in media supplemented with toxic salt concentrations (short-term salt treatment). RT-Q-PCR analysis revealed that BvpAPX expression markedly increased in leaves of plants subjected to conditions of long-term treatment with salinity, whereas BvpAPX transcript levels remained unaffected in detached leaves during short-term salt treatment. In addition, several leaf redox system parameters, such as ascorbate peroxidase activity or ascorbic acid, hydrogen peroxide, and lipid hydroperoxide concentration, were determined in the leaves of beet plants subjected to salt stress conditions.Electronic supplementary materialThe online version of this article (doi:10.1007/s11105-013-0636-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Cloning and Expression Analysis of a Gene Encoding for Ascorbate Peroxidase and Responsive to Salt Stress in Beet (Beta vulgaris)

et al. 2013

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“…The coevolution between plants and herbivores has generated a costly defensive trait, which is greatly dependent on the concentration and timing of the hormone (JA, SA, or ethylene) [17,18]. Extensive studies defining the global expression profiles upon the activation of wounding signaling pathways have been conducted using microarrays [19] and proteomic [20]. The role of such signaling hormones, integrated in the decision-making of stress response, are played by a class of Reactive oxygen species (ROS) [21].…”

Section: Introductionmentioning

confidence: 99%

Differential activation of sporamin expression in response to abiotic mechanical wounding and biotic herbivore attack in the sweet potato

et al. 2014

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BackgroundPlants respond differently to mechanical wounding and herbivore attack, using distinct pathways for defense. The versatile sweet potato sporamin possesses multiple biological functions in response to stress. However, the regulation of sporamin gene expression that is activated upon mechanical damage or herbivore attack has not been well studied.ResultsBiochemical analysis revealed that different patterns of Reactive oxygen species (ROS) and antioxidant mechanism exist between mechanical wounding (MW) and herbivore attack (HA) in the sweet potato leaf. Using LC-ESI-MS (Liquid chromatography electrospray ionization mass spectrometry analysis), only the endogenous JA (jasmonic acid) level was found to increase dramatically after MW in a time-dependent manner, whereas both endogenous JA and SA (salicylic acid) increase in parallel after HA. Through yeast one-hybrid screening, two transcription factors IbNAC1 (no apical meristem (NAM), Arabidopsis transcription activation factor (ATAF), and cup-shaped cotyledon (CUC)) and IbWRKY1 were isolated, which interact with the sporamin promoter fragment of SWRE (sporamin wounding-responsive element) regulatory sequences. Exogenous application of MeJA (methyl jasmonate), SA and DIECA (diethyldithiocarbamic acid, JAs biosynthesis inhibitor) on sweet potato leaves was employed, and the results revealed that IbNAC1 mediated the expression of sporamin through a JA-dependent signaling pathway upon MW, whereas both IbNAC1 and IbWRKY1 coordinately regulated sporamin expression through JA- and SA-dependent pathways upon HA. Transcriptome analysis identified MYC2/4 and JAZ2/TIFY10A (jasmonate ZIM/tify-domain), the repressor and activator of JA and SA signaling among others, as the genes that play an intermediate role in the JA and SA pathways, and these results were further validated by qRT-PCR (quantitative real-time polymerase chain reaction).ConclusionThis work has improved our understanding of the differential regulatory mechanism of sporamin expression. Our study illustrates that sweet potato sporamin expression is differentially induced upon abiotic MW and biotic HA that involves IbNAC1 and IbWRKY1 and is dependent on the JA and SA signaling pathways. Thus, we established a model to address the plant-wounding response upon physical and biotic damage.

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“…Although proteomic approaches have been used in the study of fruit ripening in tomato (Rocco et al, 2006), grape (Zhang et al, 2008), citrus (Muccilli et al, 2009;Perotti et al, 2011), prunus (Nilo et al, 2012), papaya (Huerta-Ocampo et al, 2012;Nogueira et al, 2012), mango (Andrade et al, 2012) and banana , there are only a few reports dealing with the response of plants to wounding (Shen et al, 2003;Chaves et al, 2009;Dafoe et al, 2009;Valcu et al, 2009;Lewandowska-Gnatowska et al, 2011;Soares et al, 2011;Barkla et al, 2013). Regarding apple proteomic studies, Guarino et al (2007) described the repertoire of proteins present in the pseudocarp tissues of three accessions of Malus × domestica Borkh.…”

Section: Introductionmentioning

confidence: 99%

Analysis of changes in protein abundance after wounding in ‘Golden Delicious’ apples

Buron-Moles

Torres

Amoako-Andoh

et al. 2014

Postharvest Biology and Technology

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A proteomic analysis of the wound response in Medicago leaves reveals the early activation of a ROS-sensitive signal pathway

Cited by 21 publications

References 43 publications

Cloning and Expression Analysis of a Gene Encoding for Ascorbate Peroxidase and Responsive to Salt Stress in Beet (Beta vulgaris)

Cloning and Expression Analysis of a Gene Encoding for Ascorbate Peroxidase and Responsive to Salt Stress in Beet (Beta vulgaris)

Differential activation of sporamin expression in response to abiotic mechanical wounding and biotic herbivore attack in the sweet potato

Analysis of changes in protein abundance after wounding in ‘Golden Delicious’ apples

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