A Proteomic Approach To Analyze Auxin- and Zinc-Responsive Protein in Rice

Abstract

Auxin and zinc are involved in callus and root formation in rice. However, details of the mechanism underlying this process and functional relation between zinc and auxin are unclear. In this study, proteins induced by auxin and zinc in rice were analyzed by a proteomic approach. Root formation on rice seedlings was promoted by 0.45 microM 2,4-D treatment and was further promoted by addition of 260 microM Zn. Microscopic observation revealed that the number of root primodia formed was significantly increased in 2,4-D- and Zn-treated seedlings than that of the control. A total of seven proteins, as analyzed by 2D-PAGE, were increased, and one protein was decreased by 2,4-D and Zn treatment. Expression of elongation factor-1beta' (EF-1beta') both at transcriptional and translational levels was particular abundant in callus and basal parts of young seedlings, and the accumulation of EF-1beta' was consistent with root formation induced by 2,4-D and Zn. Results indicate that higher level of EF-1beta' expression is necessary for auxin- and zinc-induced root formation in rice.

Ectopic expression of foxtail millet zip-like gene, SiPf40, in transgenic rice plants causes a pleiotropic phenotype affecting tillering, vascular distribution and root development

“…In another study, proteins induced by 2,4-D and zinc in rice were analyzed by proteomics. Auxin and Zn were speculated to increase the expression of components such as elongation factor-1 beta′ (EF-1β′) in protein synthesis machinery, resulting in active protein synthesis necessary for auxin and Zn-induced adventitious root formation [47]. SiPf40 might affect hormone metabolism by the same mechanism as AtIAR1, by transporting inhibitory metals out of the ER to inhibit a key enzyme related to auxin metabolism.…”

Phytotoxic mechanisms of bur cucumber seed extracts on lettuce with special reference to analysis of chloroplast proteins, phytohormones, and nutritional elements

“…Higher uptake of Na leads to nutritional imbalance in plants (Neel et al, 2002), and the accumulation of Cu inhibits the synthesis of proteins, carbohydrates, and chlorophyll as well as nutrient uptake (Xing et al, 2010). The higher concentration of Zn stimulates auxin synthesis through tryptophan (Yang et al, 2005) and inhibits plant growth. Na accumulation in plant cells interrupts uptake of other essential nutrients and water Lee, 2013b, 2014).…”

Protein Homologous to Human CHD1, Which Interacts with Active Chromatin (HMTase) from Onion Plants

“…The meristematically active parts (200 mg) isolated from onion plants were homogenized with lysis buffer containing 8 M urea, 2% NP-40, 5% β-mercaptoethanol, and 5% polyvinyl pyrrolidene, and then assayed by 2-DE (Yang et al 2005). Extracted protein samples (100 µg) were separated in the first dimension by isoelectric focusing (IEF) tube gel and in the second dimension by SDS-PAGE.…”