A Proteomics Approach to Investigating Promotive Effects of Brassinolide on Lamina Inclination and Root Growth in Rice Seedlings.

Konishi, Hirosato; Komatsu, Setsuko

doi:10.1248/bpb.26.401

Cited by 34 publications

(20 citation statements)

References 42 publications

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“…After BR treatment of Arabidopsis bul1 and dim1 mutants, microtubules reorganized and became correctly oriented, suggesting the involvement of BR in microtubule organization and cell elongation [21]. In rice proteomics, tubulin content also exhibited an evident increase upon BR treatment [22]. Therefore, the re-upregulation of alpha tubulin was considered to correspond highly to the BR-mediated growth observed in this study.…”

Section: Protein Identifications and Putative Functions In The Br-medsupporting

confidence: 53%

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A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Huang

Chu

Chen

et al. 2006

Cellular and Molecular Biology Letters

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Abstract:Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These upregulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-upregulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.

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Section: Protein Identifications and Putative Functions In The Br-medsupporting

confidence: 53%

“…Proteomics is a powerful tool that is increasingly applied in the study of almost any aspect of protein expression or function. However, little proteomics-based research has been done on BR-promoted cell growth [9]. Moreover, the correlation between BR and the stress response is not yet fully understood.…”

Section: Introductionmentioning

confidence: 99%

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Huang

Chu

Chen

et al. 2006

Cellular and Molecular Biology Letters

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“…For example, the expression patterns of eight proteins including GAPDH; GST; EF-Tu; HSP70; PDI; GSSI; ATP synthase CF1, ␤ subunit; and fructose-bisphosphate aldolase (Table IV) in H 2 O 2 -treated rice seedling leaves were observed to be similar to those in Arabidopsis mitochondria and yeast subjected to H 2 O 2 stress (25,30), implying the similar response of different species and/or tissues under H 2 O 2 stress. A particular case was GST: its abundance of protein expression was up-regulated not only by H 2 O 2 stress in this study but also by other abiotic stresses such as cold, drought, jasmonic acid, and brassinolide in rice seedling leaves (29,37,64,65). GST is known to function as a pivotal component in plant defense systems and catalyzes the reduction reaction of toxic organic hydroperox-ides by using glutathione as a cosubstrate or coenzyme (48).…”

Section: Differentially Expressed Proteins Are Largely Implicated In mentioning

confidence: 89%

Comparative Proteomics Analysis Reveals an Intimate Protein Network Provoked by Hydrogen Peroxide Stress in Rice Seedling Leaves

Wan

Liu

2008

Molecular & Cellular Proteomics

131

127

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Hydrogen peroxide (H 2 O 2 ) plays a dual role in plants as the toxic by-product of normal cell metabolism and as a regulatory molecule in stress perception and signal transduction. However, a clear inventory as to how this dual function is regulated in plants is far from complete. In particular, how plants maintain survival under oxidative stress via adjustments of the intercellular metabolic network and antioxidative system is largely unknown. To investigate the responses of rice seedlings to H 2 O 2 stress, changes in protein expression were analyzed using a comparative proteomics approach. Treatments with different concentrations of H 2 O 2 for 6 h on 12-dayold rice seedlings resulted in several stressful phenotypes such as rolling leaves, decreased photosynthetic and photorespiratory rates, and elevated H 2 O 2 accumulation. Analysis of ϳ2000 protein spots on each twodimensional electrophoresis gel revealed 144 differentially expressed proteins. Of them, 65 protein spots were upregulated, and 79 were down-regulated under at least one of the H 2 O 2 treatment concentrations. Furthermore 129 differentially expressed protein spots were identified by mass spectrometry to match 89 diverse protein species. These identified proteins are involved in different cellular responses and metabolic processes with obvious functional tendencies toward cell defense, redox homeostasis, signal transduction, protein synthesis and degradation, photosynthesis and photorespiration, and carbohydrate/energy metabolism, indicating a good correlation between oxidative stress-responsive proteins and leaf physiological changes. The abundance changes of these proteins, together with their putative functions and participation in physiological reactions, produce an oxidative stress-responsive network at the protein level in H 2 O 2 -treated rice seedling leaves. Such a protein network allows us to further understand the possible management strategy of cellular activities occurring in the H 2 O 2 -treated rice seedling leaves and provides new insights into oxidative stress responses in plants.

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“…Taken together, these results indicate that the BR-induced DREPP protein mediates BR promotion of plant growth. (58) or in mung bean (59).…”

Section: Fig 6 2-d Dige Analyses Of Br-regulated Plasma Membrane Prmentioning

confidence: 99%

Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-dimensional DIGE

Tang

Deng

Oses-Prieto

et al. 2008

Molecular & Cellular Proteomics

133

139

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Signal transduction involves posttranslational modifications and protein-protein interactions, which can be studied by proteomics. In Arabidopsis, the steroid hormone (brassinosteroid (BR)) binds to the extracellular domain of a receptor kinase (BRI1) to initiate a phosphorylation/ dephosphorylation cascade that controls gene expression and plant growth. Here we detected early BR signaling events and identified early response proteins using prefractionation and two-dimensional (2-D) DIGE. Proteomic changes induced rapidly by BR treatments were detected in phosphoprotein and plasma membrane (PM) fractions by 2-D DIGE but not in total protein extracts. LC-MS/MS analysis of gel spots identified 19 BR-regulated PM proteins and six proteins from phosphoprotein fractions. These include the BAK1 receptor kinase and BZR1 transcription factor of the BR signaling pathway. Both proteins showed spot shifts consistent with BR-regulated phosphorylation. In addition, in vivo phosphorylation sites were identified for BZR1, two tetratricopeptide repeat proteins, and a phosphoenolpyruvate carboxykinase (PCK1). Overexpression of a novel BR-induced PM protein (DREPP) partially suppressed the phenotypes of a BR-deficient mutant, demonstrating its important function in BR responses. Our study demonstrates that prefractionation coupled with 2-D DIGE is a powerful approach for studying signal transduction. Molecular & Cellular Proteomics 7:728 -738, 2008.Perception and response to extracellular signals are crucial for growth and survival of all organisms. Signals are transduced intracellularly through mechanisms that include posttranslational protein modification and protein-protein interaction. Detection of such signaling events using proteomics methods can identify proteins involved in signal transduction but is technically challenging because of the low abundance of signaling proteins. Brassinosteroids (BRs) 1 are plant hormones that play important roles in multiple plant developmental processes. Mutant plants with a defect in BR synthesis or signal transduction show a wide range of phenotypes including dwarfism, reduced fertility, light-grown morphology in the dark, and delayed senescence (1). BRs structurally resemble animal steroid hormones but act through a distinct signaling mechanism (2). Although animal steroid hormones are perceived by nuclear receptors, BRs are perceived by a cell surface receptor-like kinase named BRI1 (2, 3). BRs bind to the extracellular domain of BRI1 to activate its kinase and downstream BR signal transduction (4), which involves another receptor-like kinase named BAK1 (5, 6); a GSK3-like kinase, BIN2 (7); a phosphatase, BSU1 (8); and two transcription factors, BZR1 and BZR2 (also known as BES1) (9, 10). In the absence of BR, the inhibitory BIN2 kinase phosphorylates BZR1 and BZR2, and phosphorylation inhibits the function of BZR1 and BZR2 through multiple mechanisms, which include inhibition of DNA binding activity, degradation by the proteasome, and cytoplasmic retention by the 14-3-3 proteins that b...

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A Proteomics Approach to Investigating Promotive Effects of Brassinolide on Lamina Inclination and Root Growth in Rice Seedlings.

Cited by 34 publications

References 42 publications

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Comparative Proteomics Analysis Reveals an Intimate Protein Network Provoked by Hydrogen Peroxide Stress in Rice Seedling Leaves

Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-dimensional DIGE

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