A Pyrene Seco-Pseudonucleoside in Constructing Interaction-Sensitive Fluorescent DNA Probes

Synthesis of 3'- and 3',5'-modified oligonucleotides on functionalized silica Silochrom-2

“…Results and Discussion. A number of supports for the synthesis of oligonucleotides modified with fluorescent dyes, including fluorescein, rhodamine etc., are described in literature [13][14][15][16][17]. The preparation of polymer supports for synthesis of 3'-labeled oligonucleotides requires a bifunctional linker which allows simultaneous attachment of both reporter group and oligonucleotide.…”

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“…Dimeric phosphoramidite building blocks that enable introduction of modified internucleosidic linkages into ODNs include stereochemically pure dinucleoside-3A,5A-boranophosphates, 66 dinucleoside-3A,5A-phosphorodithioates, 67 chiral conformationally rigid dinucleoside-3A,5A-phosphates having the phosphate group tethered to C5 of the 5A-linked nucleoside, 68 and dimers containing a hydroxamate linkage, 3A-CH 2 C(NO)N(OR)-5A. 69 Non-nucleosidic phosphoramidite reagents that either allow the chain elongation [70][71][72][73] or only 5A-derivatization [74][75][76][77] on a support have also been applied to similar purposes.…”

A Convenient Solid-Phase Method for Synthesis of 3‘-Conjugates of Oligonucleotides

“…In variations of this type of scaffold, a 3-O-aminopropyl glycerol linker has been used for the preparation of 3′-fluorescein conjugates of oligonucleotides (type iii) (Theisen et al, 1992) and a hexadecyl chain has been introduced at the 3′-end via a glyceryl linker (Rait et al, 2000). Similarly, a 3′-pyrene conjugate was prepared via a 1,3-propanediol linker (type iv) (Korshun et al, 1999). All of these types of linkers introduce chirality at the linker branch point.…”