A quantitative analysis of Zidovudine containing formulation by FT-IR and UV spectroscopy

Abstract

In this study IR and UV spectroscopic procedures are describe for the quantitative determination of Zidovudine (AZT) from solid dosage form. For IR spectroscopic method (KBr disc technique) has been used and Ursodeoxycholic acid (UDCA) was used as an internal standard. The specific absorption bands at 2105 and 2931 cm À1 were chosen for AZT and UDCA respectively. In this method, beer's law was obeyed in the concentration range 0.8-2.0% w/w in KBr disc. The regression equation was found to be y ¼ 1.088x + 1.194 with correlation coefficient 0.9819, and the assay was found to be 99.8% with RSD of 2.157%. The UV spectroscopic method was also used in the quantitative determination of AZT in solid dosage form. 266 nm was chosen as l max . The regression equation was found to be y ¼ 0.0434x + 0.027, and the correlation coefficient 0.9989. The assay was found to be 99.81% with RSD of 0.273% by the UV spectroscopic method.

Surface-enhanced Raman and DFT study on zidovudine

“…Improper hydrogen bonds caused blueshift in C-H bond stretch vibrations in three conformers of zidovudine that have been studied using DFT in gas phase and in aqueous solution. Recently, Peepliwal et al [9] developed and then validated two analytical methods for the quantification of AZT containing solid dosage form by using FTIR and UV-Vis spectroscopies. AZT showed no interference from the common additives and excipients, demonstrating that the recommended procedure is well suited for the assay and evaluation of drugs in pharmaceutical preparations.…”

A process analytical technology approach for the production of fixed-dose combination tablets of zidovudine and lamivudine using near infrared spectroscopy and chemical images

“…In the scientific literature, some works can be found describing the use of HPLC and UVVis spectroscopy for the quantification of these drugs in pharmaceutical formulations [5][6][7][8][9][10][11][12]. These methods, however, are expensive, laborious, consume a considerable amount of reagent, and require many analytical manipulations.…”

Chiral Ionic Covalent Organic Framework as an Enantioselective Fluorescent Sensor for Phenylalaninol Determination

“…5 Distinct chiral drug isomers frequently demonstrate considerable variances in pharmacology, toxicology, and pharmacokinetics. 6 Just one isomer of a chiral medicine has good pharmacological activity in some situations, whereas the others are less active or even poisonous to living organisms. Thus, in the subject of chiral analysis, the development of quick and efficient chiral identification methods has always been a hot and demanding issue.…”