A quantitative approach to the classification of hypospermatogenesis in testicular biopsies for infertility

Guarch, Rosa; Pesce, Carlo; Puras, A; Lázaro, Javier

doi:10.1016/0046-8177(92)90265-5

Cited by 23 publications

(12 citation statements)

References 15 publications

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“…Moreover, in disturbed spermatogenesis, germ cells in the first meiotic prophase can show swollen or disorganized chromatin (Holstein et al, 1988 (Nistal and Paniagua, 1984;Holstein et al, 1988). In another study, there was no difference in the total number of germ cells between right and left testicular biopsies (Guarch et al, 1992). Such differences between studies confirm the importance of quantitative evaluation.…”

Section: Discussionsupporting

confidence: 49%

“…In the testicular biopsy series studied, the 4D4 labelling revealed that maturation arrest could occur at the level of either mid-pachytene primary spermatocytes or earlier as seen by the absence of 4D4 labelling in the excluded cases. In addition, as some cells escape the blockage, maturation arrest has been considered as a type of hypospermatogenesis (Honoré, 1979), and its existence as a separate entity has been questioned (Guarch et al, 1992). However, in the study reported here, significant differences were found between these two disturbances of spermatogenesis, supporting the notion that these disorders must be of different origin (Nistal and Paniagua, 1984;Skakkebaek et al, 1989).…”

Section: Discussioncontrasting

confidence: 40%

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Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Bermúdez

Escalier²,

Gallo³

et al. 1994

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Section: Discussionsupporting

confidence: 49%

Section: Discussioncontrasting

confidence: 40%

Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Bermúdez

Escalier²,

Gallo³

et al. 1994

Reproduction

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“…A reduction in tubular diameter, thickening of the tubular wall and increase in Leydig‐cell density are common in severe germ‐cell impairment, although the distribution is irregular [9]. A thickening of the seminiferous tubule and blood vessel walls could be responsible for the limited functional capacity of Sertoli cells, causing altered spermatogenesis in cases of excretory azoospermia [10].…”

Section: Discussionmentioning

confidence: 99%

Clinical and hormonal findings in testicular maturation arrest

2004

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testosterone, prolactin and oestradiol), testicular volume, and the diameter of the seminiferous tubules, were evaluated. Patients were categorized into groups according to Johnsen score, i.e. group 1 ( < 4), group 2 ( ≥ 4 and < 6) or group 3 ( ≥ 6). The clinical variables were compared in each group. RESULTSHormone levels in almost all patients were in the normal range. FSH was related to the stage of MA; the difference in FSH between groups 1 and 3 was significant ( P = 0.006). Serum levels of LH, testosterone, prolactin or oestradiol were similar in all groups. The diameter of the seminiferous tubules was less in group 1 than in group 3 ( r = 0.881, P < 0.001) and there was an inverse and linear correlation between the FSH concentration and the diameter of the seminiferous tubules (r = -0.661, P = 0.0028). CONCLUSIONSFSH is the most useful variable for evaluating the severity of MA, even when it is within the normal range. The finding that gonadal hormone concentrations are normal in patients with MA suggests that an abnormality other than a disturbance in the hypothalamo-pituitary-testicular axis is responsible for MA.

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“…In addition, although the pathophysiological role of the Ang II actions on TPMC remains to be elucidated, our data suggest that Ang II-induced TPMC growth might be, to some extent, responsible for the proliferation of those cells in human testicular disorders characterized by thickening of the tubular wall, such as varicocele (39), hypospermatogenesis (40), and cryptorchidism (41).…”

Section: Discussionmentioning

confidence: 77%

Angiotensin II Stimulates Contraction and Growth of Testicular Peritubular Myoid Cellsin Vitro

et al. 2002

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Seminiferous tubule contraction, an important step in the regulation of spermatogenesis and testicular sperm output, is regulated by several agonists. In the present paper, we investigated whether angiotensin II (Ang II) may have a place among them. In binding experiments performed to assess the presence of specific receptors in rat peritubular myoid cells (TPMC), binding of (125)I-Ang II to TPMC was saturable in a time-dependent manner. Competition binding experiments performed with Losartan and PD 123319 showed that Losartan was able to inhibit the binding of (125)I-Ang II, whereas PD 123319 was ineffective. Ang II induced a dose-dependent rise in intracellular Ca(2+). Depletion of intracellular calcium stores by thapsygargin resulted in a lower rise of intracellular calcium, and the L-type voltage-operated calcium channel (VOCC-L) blocker verapamil abolished the Ca(2+) influx in rat TPMC. Altogether, these findings indicate that the Ang II-induced increase in [Ca(2+)](i) involves both extracellular influx and Ca(2+) release from intracellular stores. Ang II induced a dose-dependent TPMC contraction, and Losartan and not PD 123319 inhibited the response. Ang II-induced contraction was inhibited by adrenomedullin, previously shown to antagonize endothelin 1-provoked contraction in those cells. Ang II elicited (3)H-thymidine DNA incorporation and proliferation in a dose-dependent manner in TPMC. Losartan and both MAPK inhibitor PD 98059 and tyrosine kinase inhibitor AG18 were able to inhibit Ang II-induced (3)H-thymidine uptake and cell proliferation. In conclusion, the present study documents that angiotensin II, the active mediator of the tissue and circulating renin-angiotensin system present in the mammalian testis, induces contraction, growth and rise in intracellular calcium in rat peritubular myoid cells via angiotensin II type 1 receptors, and suggests that Ang II is involved in the paracrine regulation of the seminiferous tubule function.

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A quantitative approach to the classification of hypospermatogenesis in testicular biopsies for infertility

Cited by 23 publications

References 15 publications

Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Proacrosin as a marker of meiotic and post-meiotic germ cell differentiation: quantitative assessment of human spermatogenesis with a monoclonal antibody

Clinical and hormonal findings in testicular maturation arrest

Angiotensin II Stimulates Contraction and Growth of Testicular Peritubular Myoid Cellsin Vitro

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