A Quantitative Test for Biotin and Observations Regarding its Occurrence and Properties

Snell, Esmond E.; Eakin, Robert E.; Williams, Roger J.

doi:10.1021/ja01858a052

Cited by 169 publications

(34 citation statements)

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“…Because several microorganisms can convert DTB to biotin (8)(9)(10)(11)(12), it is worthwhile studying the biotin activity of biotin-vitamers for various kinds of microorganisms. Biotin is an essential growth factor for a number of microorganisms (13)(14)(15)(16)(17). DTB is also required by some bacteria and yeasts (3,15,18).…”

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Microbiological activity of biotin-vitamers.

Ohsugi

Imanishi

1985

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe growth requirement or growth-promoting effect of biotin-vitamers on bacteria and yeasts was investigated. Biotin, dethiobiotin and biocytin (N-e-biotinyl-L-lysine) were shown to be required for growth in a number of bacteria and yeasts. The biological activity of dethiobiotin was relatively higher than that of biotin, but was negative for lactic acid bacteria. Biocytin had high activity for Bacillus subtilis (natto), Debaryomyces japonicus and Hansenula capsulate. The biotin activity of 7-keto-8-aminopelargonic and 7,8-diaminopelargonic acids was low or negligible for bacteria but relatively high for yeasts such as the genera of Endomyces, Endomycopsis and Saccharomyces. Pimelic, pelargonic and pelargonylhydroxamic acids had no growth requirement fo r or growth-promoting effect on any of the bacteria or yeasts tested.

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“…Biotin is an essential growth factor for a number of microorganisms (13)(14)(15)(16)(17). DTB is also required by some bacteria and yeasts (3,15,18). However, the biotin activity of DAPA and KAPA is low or negligible for Saccharomyces cerevisiae (18)(19)(20) and Bacillus subtilis (15).…”

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Microbiological activity of biotin-vitamers.

Ohsugi

Imanishi

1985

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Desthiobiotin synthesized was determined by assay with Saccharomyces cerevisiae. 6 ) The desthiobiotin yields at 0,0.01,0.03,0.1 and 0.3 ml Sample I were 0, 0.015, 0.03, 0.11 and 0.28 /lg per ml, respectively. This result shows that the formation of desthiobiotin is directly proportional to the amount of Sample I introduced into the assay solution.…”

Section: Received March 16 1981mentioning

confidence: 90%

Formation of Pimelic and Azelaic Acids, Biotin Intermediates, Derived from Oleic Acid byMicrococcussp.

Ohsugi¹,

Inoue²

1981

Agricultural and Biological Chemistry

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“…On addition of the filtrate fraction the medium now became suitable for assay of the Norit eluate factor, which I succeeded in purifying about WOO-fold before leaving Madison. The active principle was acidic (it was extracted by butanol from acidic but not from basic aqueous solution), but showed behavior toward precipitants that strongly resembled the purine bases and basic amino acids (17a,b shown that biotin (a) replaced biotic acid completely (19), (b) replaced a then unidentified growth factor for Clostridium butylicum (20) , and (c) together with the newly isolated vitamin, pyridoxine, completely replaced the filtrate factor for L. casei. [Moeller had reported in 1938 that both pyridoxine and biotin were required by Lactobacillus plantarum (21a,b).]…”

Section: The Norit Eluate Factormentioning

confidence: 99%

FROM BACTERIAL NUTRITION TO ENZYME STRUCTURE: A Personal Odyssey

Snell

1993

Annu. Rev. Biochem.

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As a former editor or member of the Editorial Board of the Annual Review of Biochemistry I helped to select authors of the prefatory chapters for more than 20 years. Now I am asked to write one myself. The first such chapter appeared in 1953 with the prefatorial statement: “This is the first of a series in which it is the hope of the Editorial Committee that our elders in biochemistry will give us through chapters of a historical and philosophical character the benefit of their long years of experience in biochemistry.” With one exception, the chapters have appeared regularly ever since. Their authors comprise a group I am proud to join even while harboring some doubts about really belonging. Their autobiographical accounts have added much to our understanding of how and why they became biochemists, and how biochemistry itself developed. I shall continue in this tradition.

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A Quantitative Test for Biotin and Observations Regarding its Occurrence and Properties

Cited by 169 publications

References 1 publication

Microbiological activity of biotin-vitamers.

Microbiological activity of biotin-vitamers.

Formation of Pimelic and Azelaic Acids, Biotin Intermediates, Derived from Oleic Acid byMicrococcussp.

FROM BACTERIAL NUTRITION TO ENZYME STRUCTURE: A Personal Odyssey

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