2016
DOI: 10.1016/j.mimet.2016.04.002
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A quick colorimetric method for total lipid quantification in microalgae

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Cited by 92 publications
(51 citation statements)
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“…Color intensity varies between different lipids due to structural differences in fatty acids. A higher unsaturated fatty acids content leads to higher intensities; however, the presence of polyunsaturated fatty acids does not translate in a more intense color because of steric hindrance [191]. For example, triolein resulted in higher color intensity compared to the polyunsaturated eicosapentaenoic and docosahexaenoic acids, whereas unsaturated fatty acids did not react with the SPV reagent, as expected.…”
Section: Colorimetric Detectionsupporting
confidence: 54%
See 1 more Smart Citation
“…Color intensity varies between different lipids due to structural differences in fatty acids. A higher unsaturated fatty acids content leads to higher intensities; however, the presence of polyunsaturated fatty acids does not translate in a more intense color because of steric hindrance [191]. For example, triolein resulted in higher color intensity compared to the polyunsaturated eicosapentaenoic and docosahexaenoic acids, whereas unsaturated fatty acids did not react with the SPV reagent, as expected.…”
Section: Colorimetric Detectionsupporting
confidence: 54%
“…For example, triolein resulted in higher color intensity compared to the polyunsaturated eicosapentaenoic and docosahexaenoic acids, whereas unsaturated fatty acids did not react with the SPV reagent, as expected. [191]. This observation led the authors to conclude that the reaction was affected by the samples' concentration and chemical structure.…”
Section: Colorimetric Detectionmentioning
confidence: 99%
“…The advantages of this technique are that it is able to measure lipid content rapidly and simply and requires only a small amount of target sample. Thus, this method has been employed for rapid quantification of the intracellular lipid contents of some microalgae (Hao et al 2013, Mishra et al 2014, Byreddy et al 2016.…”
Section: Color Reaction Of Four Dinoflagellate Species In Different Cmentioning
confidence: 99%
“…The advantages of this technique are that it is able to measure lipid content rapidly and simply and requires only a small amount of target sample. Thus, this method has been employed for rapid quantification of the intracellular lipid contents of some microalgae (Hao et al 2013, Mishra et al 2014, Byreddy et al 2016.In this study, we applied a modified SPV method for quantifying the lipid content of dinoflagellate species. Furthermore, using this method, we analyzed the lipid content of 9 dinoflagellate species including phototroph- ) was supplied to the predator cells every day for 5 days, but not on the last day, in order to eliminate all yeast from the O. marina culture medium.…”
mentioning
confidence: 99%
“…An increase in RUE indicates that the MPB are efficiently assimilating the identified nutrient (nitrogen in this case) and converting it to biomass. Aliquots of tissue from freeze-dried bivalves and sediments were used to assess total lipids using a modified Bligh and Dyer (1959) and lipid content determined using the sulfo-phospho-vanillin (SPV) spectrophotometric method (Byreddy et al, 2016). Total fatty acid (TFA) composition was determined following a one-step direct transesterification method (Lepage and Roy, 1986;Zárate et al, 2016).…”
Section: Biochemical Propertiesmentioning
confidence: 99%