A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes

Goulas, Vlasios; Andreou, T. Anisimova; Moditi, C. Angastinioti; Tzamaloukas, Ouranios

doi:10.22358/jafs/65708/2014

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…However, many studies agree that a precise assessment of SMX effects is not possible without a thorough analysis of its environmental distribution. While standard lab based analytical platforms like high pressure liquid chromatography (HPLC) and mass spectrometry (MS) are readily available to analyse and quantify SMX in environment samples, their labour, cost, and time intensive technology limits a widespread monitoring [11][12][13] . This raises the need for the development of new, fast, and cost-effective technologies enabling point-of-use analysis to monitor and quantify the distribution of SMX and other antibiotics.…”

Section: Introductionmentioning

confidence: 99%

“…While standard lab based analytical platforms like high pressure liquid chromatography (HPLC) and mass spectrometry (MS) are readily available to analyse and quantify SMX in environment samples, their labour, cost, and time intensive technology limit a widespread monitoring. [11][12][13] This raises the need for the development of new, fast, and costeffective technologies enabling point-of-use analysis to monitor and quantify the distribution of SMX and other antibiotics. Over the last few years, various new approaches for sulfonamide detection were published, covering different methods such as immunochemical detection, 14 colorimetric approaches 15 and electrochemical technologies.…”

Section: Introductionmentioning

confidence: 99%

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Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles

et al. 2023

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles

et al. 2023

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“…The diyode array detector was used determination for TMP and SMZ [24]. Goulas et al (2014) applied HPLC method for the simultaneous determination of sulfadimidine, sulfadiazine, sulfamethoxazole and trimethoprim antibiotics in medicated feeds has been developed analysis method to commercial feed premixes after the ultrasound-assisted extraction prosedure [25]. The aim of this study was to investigate simultaneous determination of SCP and TMP combination in veterinary formulations by HPLC.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc

Yuvali

Narin

2020

Cumhuriyet Science Journal

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In this study, a simple, sensitive, precise, rapid and reversed-phase high performance liquid chromatographic method with ultraviolet detection for simultaneous determination of Sulfachloropyridazine and Trimethoprim is developed and applied commercial veterinary formulation for chromatographic separation, Thermoscientific Hypersil-C18, reverse phase column was used. Separation was done using acetonitrile: pH 3.0 buffer solution (30:70, v/v) at 0.8 ml min -1 flow rate. Analysis was done 272.0 nm at ultraviyolet detector. Under optimum chromatographic conditions, retention time of Sulfachloropyridazine and Trimethoprim were determined as 4.37 and 2.60 minute. The proposed method was linear in the range 1.0-100.0 μg ml -1 for Sulfachloropyridazine and 0.5-40.0 μg ml -1 for Trimethoprim. The Limit of detection of Sulfachloropyridazine and Trimethoprim are 0.05 μg ml -1 , 0.18 μg ml -1 , respectively. The method which is rapid, simple and does not require any separation step, has been successfully applied to assay of commercial dosage forms containing Sulfachloropyridazine and Trimethoprim.

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“…TMP inhibits the bacterial enzyme more than the human enzyme. Literature survey reveals that only a few selected spectrophotometric [6][7][8], HPLC [10][11], and HPLC-LC-MS [18], methods were reported for the estimation of SDA and TMP individually, UVVisible [9], HPLC [12][13][14][15][16][17], as a combination with other drugs in bulk and biological samples. There are two HPLC [19][20], one stability indicating HPLC [21], and four LC-MS [22][23][24][25] methods for the estimation of SDA and TMP as a combination in bulk and biological samples.…”

Section: Introductionmentioning

confidence: 99%

Uv Spectrophotometry Method Development and Validation of Sulfadiazine and Trimethoprim in Combined Dosage Form

Pokala

Kumari

Bollikola

2018

Int J Pharm Pharm Sci

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Objective: A new, simple, sensitive and economical UV spectrophotometric method was developed for the simultaneous analysis of Sulfadiazine [SDA] and Trimethoprim [TMP] in pharmaceutical formulations.Methods: This UV method was developed with methanol as solvent. The wavelengths selected for analysis in the present method were 265 nm for TMP and 289 nm for SDA. Teccomp UV-2301 double beam UV/Vis spectrophotometer was used to carry out spectral analysis and the data was recorded by Hitachi software.Results: Linearity was found to be within the concentration range of 2-9 µg/ml TMP and 9.08-41 µg/ml of SDA. Accuracy of the method was determined by recovery studies. Percentage recovery was found to be 98.20-99.25 for TMP with a % RSD of 0.338, 0.506 and 0.510 for three spiked levels. % RSD was found to be 0.229 and 0.380; 0.212 and 0.328 for SDA, TMP in intra and inter-day precision respectively. The % RSD value in ruggedness was found to be 0.440 for SDA and 0.569 for TMP. Conclusion:The advantages of this method for analytical purposes lie in the rapid determination, its cost-effectiveness, easy preparation of the sample, good reproducibility. In addition to this, the present method can be recommended for simultaneous determination of SDA and TMP in routine quality control analysis in combined drug formulations.

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A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes

Cited by 6 publications

References 19 publications

Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles

Development of a synthesis strategy for sulfamethoxazole derivatives and their coupling with hydrogel microparticles

Simultaneous determination of sulfachloropyridazine and trimethoprim in veterinary formulations by hplc

Uv Spectrophotometry Method Development and Validation of Sulfadiazine and Trimethoprim in Combined Dosage Form

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