A Rapid, Quantitative GLC Method for the Simultaneous Determination of Nicotine and Cotinine

Abstract

Published gas-liquid chromatographic (GLC) methods for the determination of nicotine and cotinine have proved impractical for the analysis of a large number of clinical samples. Significant improvements over other methods have been achieved, being low sample volume (0.5 mL plasma), rapid two-step extraction from plasma, no evaporation step, and good separation. The lower limits of sensitivity for nicotine and cotinine were 1 and 5 ng/mL, respectively. The method was validated by the analysis of plasma samples from cigarette-smoking volunteers. The method described permits the quick, routine determination of nicotine and cotinine in a large number of samples.

Measuring tobacco smoke exposure: quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods

“…Based on the above-mentioned attributes, gas chromatography [34,65,68,74,76,79,[93][94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110] is the method of choice for monitoring ETS exposure by quantifying cotinine concentration in plasma/saliva, particularly when concentration levels are less than 1 ng ml −1 . A related technique, GC-MS involves coupling a gas chromatograph with a mass spectrometer, and has been recognized as an ultimate standard of reference in analysis [111].…”

Determination of nicotine and cotinine in rat plasma by liquid chromatography–tandem mass spectrometry

“…It is important to avoid the use of glassware because nicotine from the air or from the sample absorbed to it. Initially, protein precipitation was performed by the addition of TCA as described in literature [8]. By switching from TCA to a solution of HCl and NaCl as described by Jung et al [4], higher absolute recoveries were observed.…”

Effects of cigarette smoking on short‐term variability of blood pressure in smoking and non smoking healthy volunteers

“…Each blood sample was centrifuged immediately at 4 "C for 10 min, and then frozen at -20 "C until assay. The plasma concentration of nicotine was determined by gas chromatography and mass spectrometry [20]. The results are reported as mean f SD for haemodynamic parameters (normal distribution) and as median with 95% confidence interval (binomial-based method) for spectral analysis parameters (non-normal distribution).…”