A ratiometric electrochemical aptasensor for sensitive detection of protein based on aptamer–target–aptamer sandwich structure

Yu, Ping; Zhou, Jun; Wu, Liang; Xiong, Erhu; Zhang, Xiaohua; Chen, Jinhua

doi:10.1016/j.jelechem.2014.08.034

Cited by 32 publications

(15 citation statements)

References 50 publications

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“…Approaches towards the non-amplified and amplified ratiometric electrochemical detection of thrombin, a coagulation protein, can provide the perfect illustration of the benefits afforded by amplification. Firstly, Zhang and Chen et al used a dual-aptamer sandwich structure to generate a non-amplified, ratiometric electrochemical thrombin aptasensor (Figure 14a) [52]. A double-stranded DNA was used as the solid-support on a AuE to which a thrombin-specific aptamer was appended and labelled at its overhanging 5 end with MB.…”

Section: Amplification Strategiesmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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Electrochemical biosensors are an increasingly attractive option for the development of a novel analyte detection method, especially when integration within a point-of-use device is the overall objective. In this context, accuracy and sensitivity are not compromised when working with opaque samples as the electrical readout signal can be directly read by a device without the need for any signal transduction. However, electrochemical detection can be susceptible to substantial signal drift and increased signal error. This is most apparent when analysing complex mixtures and when using small, single-use, screen-printed electrodes. Over recent years, analytical scientists have taken inspiration from self-referencing ratiometric fluorescence methods to counteract these problems and have begun to develop ratiometric electrochemical protocols to improve sensor accuracy and reliability. This review will provide coverage of key developments in ratiometric electrochemical (bio)sensors, highlighting innovative assay design, and the experiments performed that challenge assay robustness and reliability.

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Section: Amplification Strategiesmentioning

confidence: 99%

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Spring

Goggins²,

Frost

2021

Molecules

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“…Ratiometric electrochemical sensing can be achieved through either tagging both termini of nucleic acid capture probes bound to electrode surfaces with redox labels that have different oxidation potentials (29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41), manipulating host-guest or metal binding interactions on electrode surfaces with electrochemically distinguishable redox probes (42)(43)(44), or the use of redox-active probes which demonstrate a unique shift in oxidation potential selectively in the presence of the analyte (45)(46). For analytes that display target catalysis as a means of amplification within diagnostic assays, we have found the latter offers a number of benefits, such as improved reaction kinetics, since probes are not surface bound, and ease of analysis, as electrodes do not require modification prior to detection (47)(48).…”

Section: Ratiometric Electrochemical Detection Of Palladiummentioning

confidence: 99%

Ratiometric electrochemical detection of Pd•••π interactions: application towards electrochemical molecular logic gates

Goggins

Stark

Naz³

et al. 2017

Supramolecular Chemistry

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The widespread and large scale use of platinum group metals, especially palladium, in a wide variety of industrial applications has seen their levels in wastewater streams, roadside dust and even pharmaceuticals significantly rise over recent years. Due to the possible environmental damage and potential health risk this may cause, there is now substantial demand for inexpensive, efficient and robust methods for the detection of palladium. Based upon self-immolative linker technologies, we have designed and synthesised a number of allyl ether-functionalised electrochemical probes to determine the optimum probe structure required to deliver a ratiometric electrochemical detection method capable of achieving a limit of detection of <1mg/mL within 20 minutes through the use of disposable screen-printed carbon electrodes. Combined with an enzymatic assay, this method was then used to achieve a proof-of-principle ratiometric electrochemical molecular logic gate.

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“…These conformational changes resulted in a decrease in the oxidation peak current of MB (I MB ) and an increase in that of Fc (I Fc ), and the logarithmic value of I Fc /I MB was shown to be linear with the logarithm of thrombin concentration. An LOD of 170 pM was achieved, in the linear range of 1 nM to 600 nM [62]. Several label-free aptasensors for thrombin detection coupled with electrochemical [117,118] or optical detection [119,120] were reported.…”

Section: Cardiac Biomarkersmentioning

confidence: 99%

Immunosensors

Cristea¹,

Florea²,

Tertiş³

et al. 2015

Biosensors - Micro and Nanoscale Applications

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Immunosensors are solid-state devices in which the immunochemical reaction is coupled to a transducer. They form one of the most important classes of affinity biosensors based on the specific recognition of antigens by antibodies to form a stable complex, in a similar way to immunoassay. Depending on the type of transducer there are four types of immunosensor: electrochemical, optical, microgravimetric and thermometric. The most commonly used bioelements for the development of electrochemical immunosensors are antibodies (Ab), followed by aptamers (Apt) and, in the last five years, microRNA (miRNA). In order to perform an early diagnosis, a method that is able to measure peptides and proteins directly in a sample, without any sample pre-treatment or any separation, is preferred. This direct detection can be performed with methods making use of the specific interaction of proteins with Ab, Apt and miRNA. The recent developments made in the immunosensor field, regarding the incorporation of nanomaterials for increased sensitivity, multiplexing or microfluidic-based devices, may have potential for promising use in industry and clinical analysis. Some examples of assays for several commercially available biomarkers will be presented. The main application fields, beside biomedical analysis, are drug abuse control, food analysis and environmental analysis.

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A ratiometric electrochemical aptasensor for sensitive detection of protein based on aptamer–target–aptamer sandwich structure

Cited by 32 publications

References 50 publications

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Ratiometric Electrochemistry: Improving the Robustness, Reproducibility and Reliability of Biosensors

Ratiometric electrochemical detection of Pd•••π interactions: application towards electrochemical molecular logic gates

Immunosensors

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