A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

Abstract

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

Stimulus Response of TPE-TS@Eu/GMP ICPs: Toward Colorimetric Sensing of an Anthrax Biomarker with Double Ratiometric Fluorescence and Its Coffee Ring Test Kit for Point-of-Use Application

“…As a dye molecule with prominent AIE characteristics, tetraphenylethylene (TPE) and its derivatives have drawn growing interest in many fields. , Besides the aggregation-induced emission (AIE), TPE could also exhibit monomer emission (ME) upon partial fixation of the rotatable substituents. − The state change-tuned two types of emissions mean that a new form of ratiometric sensing is possible. , In our paper, water-soluble tetra­(4-sulfophenyl)­ethylene (TPE-TS) with one hydrophobic core and four hydrophilic chains was selected as the guest and encapsulated into the Eu/GMP ICP host to obtain TPE-TS@Eu/GMP ICP nanoparticles. First, the morphology of the TPE-TS@Eu/GMP ICP nanoparticles was characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis.…”

“…42−44 The state change-tuned two types of emissions mean that a new form of ratiometric sensing is possible. 45,46 and four hydrophilic chains was selected as the guest and encapsulated into the Eu/GMP ICP host to obtain TPE-TS@ Eu/GMP ICP nanoparticles. First, the morphology of the TPE-TS@Eu/GMP ICP nanoparticles was characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis.…”

Efficient Imidazolium‐Biomolecule Interaction‐Assisted Amplified Quenching for Ultrasensitive Detection of Heparin

“…Upon exposure of 1 to incremental quantities of HP, its fluorescence intensity was gradually quenched and showed an upward quenching profile with very high SV quenching constant of K SV =1.17×10 8 M −1 in buffer solution as shown in Figure 4b and 4c, whereas the fluorescence in MeOH was not effectively quenched with a much lower gradient ( K SV =3.87×10 4 M −1 ). The high SV constant in this study is not only an approximately 100‐fold increase over that of previously reported self‐assembled micellar systems by our group, but is also significantly higher compared to other self‐assembled probes, conjugated polymers, and nanoparticles reported to date [9,15–20] . In addition, the analytical detection limit (ADL) of 1 is less than approximately 2.8×10 −10 M, which is among the highest reported to date (Table S1).…”

Activity‐based smart AIEgens for detection, bioimaging, and therapeutics: Recent progress and outlook

“…A new sensing strategy based on a micelle was developed by Lee and coworkers for quantitatively detecting the activity of ALP in human serum. [180] Compared with the control probe 70, introduction of a tetraethylene glycol unit on AIEgen 71 resulted in formation of micelles of the ALP-catalyzed products in the solution, resulting in a ratiometric monitoring behavior with a detection limit of below 0.034 mU/mL. Based on a chalcone derivative, Tang and Liu designed a ratiometric AIEgen 72 for tracking ALP activity.…”