2009
DOI: 10.1104/pp.109.147439
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A Real-Time Fluorogenic Assay for the Visualization of Glycoside Hydrolase Activity in Planta

Abstract: There currently exists a diverse array of molecular probes for the in situ localization of polysaccharides, nucleic acids, and proteins in plant cells, including reporter enzyme strategies (e.g. protein-glucuronidase fusions). In contrast, however, there is a paucity of methods for the direct analysis of endogenous glycoside hydrolases and transglycosidases responsible for cell wall remodeling. To exemplify the potential of fluorogenic resorufin glycosides to address this issue, a resorufin b-glycoside of a xy… Show more

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Cited by 24 publications
(28 citation statements)
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References 62 publications
(87 reference statements)
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“…We had previously presented XXXG-Res as a new probe for the real-time visualization of XEH activity in situ (Ibatullin et al, 2009). In connection with the xth31/xth32 double knockout line, comparative analysis versus the Col-0 wild type indicated suppression of XEH in distinct zones of the root and hypocotyl of the mutant.…”
Section: Discussionmentioning
confidence: 99%
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“…We had previously presented XXXG-Res as a new probe for the real-time visualization of XEH activity in situ (Ibatullin et al, 2009). In connection with the xth31/xth32 double knockout line, comparative analysis versus the Col-0 wild type indicated suppression of XEH in distinct zones of the root and hypocotyl of the mutant.…”
Section: Discussionmentioning
confidence: 99%
“…In light of the minor effects of AtXTH31 and AtXTH32 on Arabidopsis development, we directed our attention to the analysis of potential changes in XET and XEH enzyme activities in situ using specific fluorescent (Vissenberg et al, 2000) and fluorogenic (Ibatullin et al, 2009) probes, respectively. Based on the close homology of AtXTH31 and AtXTH32 as group III-A members, the double knockout line was examined directly to avoid potential complications due to functional compensation.…”
Section: Reverse Genetics Analysis Of Atxth31 and Atxth32mentioning
confidence: 99%
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