A Real‐time PCR Assay for Quantitative and Accurate Assessment of Fungicide Effects on Mycosphaerella graminicola Leaf Blotch

Abstract

A real-time PCR

Accuracy of Real-Time PCR to Study Mycosphaerella graminicola Epidemic in Wheat: From Spore Arrival to Fungicide Efficiency

“…Although all fungicide treatments resulted in a significant DNA reduction, compared with a nontreated control, later applications of prothioconazole, at 7 to 10 dpi, resulted in a high loss of efficiency ( 50%). These results are in agreement with those reported by (Godwin et al, 1999) and (Guo et al, 2007), namely that prothioconazole has a significant inhibitive effect against spore germination and postgermination, and could have a good curative effect against M. graminicola when applied at up to 20% of the latent period. However, most fungicides work best when applied early in the infection cycle, prior to visual symptom expression (O'Reilly et al, 1988).…”

“…The association of specific chemistries and a fluorescent reporter molecule (TaqMan chemistry) with PCR has permitted the development of real-time PCR (Schena et al, 2004). This technique is an accurate and reliable tool for many phytopathological studies (Guo et al, 2006(Guo et al, & 2007Fraaije et al, 1999;Schena et al, 2004). Real-time PCR analysis, also referred to as quantitative PCR (qPCR), can provide a deeper insight into host-pathogen interactions by detecting the primary inoculum, general infection level and genotypes, depending on the specific target gene.…”

Real-time PCR to study the effect of timing and persistence of fungicide application and wheat varietal resistance onMycosphaerella graminicolaand its sterol 14α-demethylation-inhibitor-resistant genotypes

“…These results are in agreement with those reported by Godwin et al . and Guo et al ., namely that prothioconazole has a significant inhibitive effect against spore germination and post‐germination, and could have a good curative effect against M. graminicola when applied at up to 20% of the latent period. However, most fungicides work best when applied early in the infection cycle, prior to visual symptom expression .…”

“…The association of specific chemistries and a fluorescent reporter molecule (TaqMan chemistry) with PCR has permitted the development of real‐time PCR . This technique is an accurate and reliable tool for many phytopathological studies . Real‐time PCR analysis, also referred to as quantitative PCR (qPCR), can provide a deeper insight into host–pathogen interactions by detecting the primary inoculum, general infection level and genotypes, depending on the specific target gene.…”

Protective and curative efficacy of prothioconazole against isolates of Mycosphaerella graminicola differing in their in vitro sensitivity to DMI fungicides

“…Real‐time PCR has been widely described as a precise, reproducible and reliable method to quantify M. graminicola before the appearance of visible symptoms during the incubation period 7, 17–19. The results for the untreated control in the present two experiments showed that the isolate ST38, which produced the lowest disease symptoms, always had a DNA amount higher than the two other isolates TO249 and TO252.…”