A red-emitting fluorescent probe for the detection of Hg²⁺ in aqueous medium, living cells and organisms with a large Stokes shift

Abstract

A red-emitting fluorescent probe has been developed for the selective and sensitive detection of Hg2+. With the addition of Hg2+, the solution of probe 1 displayed a remarkable fluorescence enhancement (102 fold) with λemmax = 625 nm and a large Stokes shift (150 nm). The detection limit of this probe was as low as 7.1 nM based on S/N = 3. This probe exhibited a good performance in detecting Hg2+ in real water samples, living cells and organisms.

Aggregation-induced emission enhancement (AIEE)-active tetraphenylethene (TPE)-based chemosensor for Hg2+ with solvatochromism and cell imaging characteristics

“…[4][5][6][7][8] Additionally, it can be accumulated through the food chain in the human body, consequently giving rise to several deleterious effects such as central nervous system defects, kidney damage, endocrine system disease and so on. [9][10][11][12] Although many governments around the world have adopted strict regulations to limit Hg 2+ emission, the global Hg 2+ pollution caused by human activities is still serious. 13,14 Therefore, it is highly desirable to develop a new method for the detection of Hg 2+ with high selectivity and sensitivity.…”

Synthesis of Sulfonylhydrazone Type Probe with High Selectivity for Rapid Detection of Mercury and Its Application in Adsorption and HeLa Cell

“…At present, traditional detection methods of mercury ions, including atomic absorption spectrometry, atomic fluorescence spectrometry, electrochemical method and inductive coupling, mostly depend on large apparatus, profes-sional operators and involve high costs, which make these unsuitable for on-site detection of mercury ion. [5][6][7][8][9][10][11] Therefore, we need to find a simple method to detect mercury ions. However, the spectrometric method has the advantages of simplicity, rapidity, low cost and high sensitivity, which is used in various fields.…”

Visualizations of Mercury Methylation and Dynamic Transformations by In Vivo Imaging

“…Fluorescence (FL) imaging, an important imaging method for biological samples, [ 13,14 ] offers powerful reagents to interrogate the physiology and pathology on species in their native environments with minimal perturbation to living systems. [ 15–17 ] Therefore, the ideal pathway for the in vivo detection of Hg(II) or MeHg could be FL imaging by dual‐response probes, [ 1,18–23 ] with biocompatibility and infrared (near‐infrared)‐emitting properties. [ 24–35 ] Nevertheless, previously reported probes always showed Hg(II) and MeHg emission at the same wavelength, [ 36 ] making simultaneous in vivo monitoring of both ions with selective signals difficult.…”