A reference measurement procedure for amino acids in blood using isotope dilution ultra-performance liquid chromatography-tandem mass spectrometry

Kim, Juok; Tran, Thi Thanh Huong; Hong, Seon-Pyo; Jeong, Ji‐Seon

doi:10.1016/j.jchromb.2017.04.027

Cited by 20 publications

(15 citation statements)

References 28 publications

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“…The RSDs between spots of the six AAs in the two batches were found to be 0.8–1.6% (low) and 0.9–2.1% (high) from whole spot sampling ( Table 1 ). The variations showed a similar range with other CRM batches made with liquid phase matrices such as plasma and serum; 24 , 25 therefore, it can be said that the samples were sufficiently homogenized. Table 1 and Supporting Information Figure S3 summarize the measurement results and breakdown of the measurement uncertainties in the two batches with a coverage of 95% confidence.…”

Section: Resultssupporting

confidence: 65%

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Development of Certified Reference Material for Amino Acids in Dried Blood Spots and Accuracy Assessment of Disc Sampling

et al. 2022

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To achieve the measurement reliability of amino acids used as diagnostic markers in clinical fields, establishing a reference measurement system is required, in which certified reference materials (CRMs) are an essential step in the hierarchy of measurement traceability. This study describes the development of dried blood spot (DBS) CRMs for amino acid analysis with complete measurement traceability to the International System of Units (SI). Six essential amino acids—proline, valine, isoleucine, leucine, phenylalanine, and tyrosine—were analyzed using isotope-dilution liquid chromatography–mass spectrometry (ID-MS). For minimizing measurement bias and uncertainty overestimation, whole spots with 50 μL of whole blood were adopted in the certification. The between-spot homogeneities by whole spot sampling were lower than 2.1%. The relative expanded uncertainties of the six amino acids in the developed DBS CRMs were lower than 5.7% at 95% confidence. The certified values are traceable to SI through both gravimetric preparation and the primary method in certification, ID-MS. Comparison among DBS testing laboratories revealed discrepancies between the whole spot and disc sampling methods. The actual sampling volume was accurately estimated by weighing, which revealed the possibility of underestimation in routine DBS testing. The candidate CRMs can support the standardization of DBS testing for amino acids through the qualification and validation of many kinds of measurement procedures to compensate the measurement bias caused by matrix-specific sampling error.

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Section: Resultssupporting

confidence: 65%

“…Measurement uncertainty estimation was performed in accordance with ISO/IEC Guide 98-3, the Guide to the Expression of Uncertainty in Measurement (GUM). 24 , 25 …”

Section: Methodsmentioning

confidence: 99%

Development of Certified Reference Material for Amino Acids in Dried Blood Spots and Accuracy Assessment of Disc Sampling

et al. 2022

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“…Equations Used for GC-MS and UPLC-MS/MS Analysis. VSC and SMM/Met concentrations were determined in accordance with methods described by Rotsatchakul and co-workers and Kim and co-workers 22,23 using MS response factors and quantitative ion areas for each compound relative to the internal standard:…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…VSC and SMM/Met concentrations were determined in accordance with methods described by Rotsatchakul and co-workers and Kim and co-workers , using MS response factors and quantitative ion areas for each compound relative to the internal standard: where C i and A i are the concentration and quantitative ion peak area of compound i, respectively. C is and A is are the concentration and quantitative ion peak area of the labeled internal standard, respectively.…”

Section: Methodsmentioning

confidence: 99%

Identification of Cooked Off-Flavor Components and Analysis of Their Formation Mechanisms in Melon Juice during Thermal Processing

Luo

Pang

et al. 2018

J. Agric. Food Chem.

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Cooked off-flavor components were identified, and their formation mechanisms were studied in heat-treated melon juices. When flavor dilution analysis methods and odor activity values were used to evaluate the cooked off-flavor in heat-treated melon juice, four volatile sulfide compounds (VSCs) were identified as contributors to the cooked off-flavor: dimethyl disulfide (DMDS), dimethyl trisulfide (DMTS), dimethyl sulfide (DMS), and 3-(methylthio)propanal (MTP). The cooked off-flavor intensities of heated juices from thick-skinned melons were stronger than those in juices from thin-skinned melons. We conducted a comparative analysis of VSCs before and after heat treatment by adding unlabeled and labeled S-methylmethionine (SMM) and/or methionine (Met) to the original melon juices. DMS and MTP were formed from SMM and Met through nucleophilic substitution and Strecker degradation, respectively. DMDS and DMTS were partly formed through the oxidative degradation of methanethiol produced from Met. Moreover, SMM could accelerate degradation of Met by increasing the amount of dicarbonyl compounds during heat treatment.

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“…Amino acids as biosynthetic precursors of many essential molecules involve in a variety of processes of life metabolism [40]. The effective analysis of amino acids in biological fluids which are high salt matrices will promote the clinical diagnosis and monitoring the related disorders.…”

Section: Separation Of Amino Acids In High Salt Matrixmentioning

confidence: 99%

Study on the separation mechanism of solid‐substrate electrospray ionization mass spectrometry

Shi

et al. 2021

J of Separation Science

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Solid‐substrate electrospray ionization mass spectrometry is an important ambient ionization technology to simplify mass spectrometry analysis. Nowadays, its separation application has been reported increasingly, however, the detailed separation mechanism is still indistinct although the chromatographic effect was reported as a possible factor. In this study, substrate‐filled capillary electrospray ionization mass spectrometry was developed as an ideal model to investigate the separation mechanism using over thirty small molecules (neutral, basic, and weakly acidic) as model compounds with C18‐bonded silica gel and silica gel as the substrates. The chromatographic effect was validated by the negative t‐value of oil‐water distribution coefficient, and the electric field effect was verified by the paired t‐test (p < 0.01) between the retention times at 5.5 and 4.0 kV. A differential equation was proposed to quantify the compound retention under electric field. The quantitative method was validated to rapidly quantify proline (31.88 μg/mL) and hydroxyproline (20.71 μg/mL) in plasma with acceptable selectivity and accuracy. In conclusion, the separation mechanism of solid‐substrate electrospray ionization mass spectrometry was the combination of the chromatographic and electric field effects, which could provide theoretical guidance for the separation optimization, and also promote its applications in biological, pharmaceutical, forensic, food and environmental analyses, etc.

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A reference measurement procedure for amino acids in blood using isotope dilution ultra-performance liquid chromatography-tandem mass spectrometry

Cited by 20 publications

References 28 publications

Development of Certified Reference Material for Amino Acids in Dried Blood Spots and Accuracy Assessment of Disc Sampling

Development of Certified Reference Material for Amino Acids in Dried Blood Spots and Accuracy Assessment of Disc Sampling

Identification of Cooked Off-Flavor Components and Analysis of Their Formation Mechanisms in Melon Juice during Thermal Processing

Study on the separation mechanism of solid‐substrate electrospray ionization mass spectrometry

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