A reusable neurotransmitter aptasensor for the sensitive detection of serotonin

Geng, Xue; Zhang, Mengtian; Long, Hongyan; Hu, Ziheng; Zhao, Biying; Feng, Lingyan; Du, Jiangyan

doi:10.1016/j.aca.2020.11.010

Cited by 31 publications

(17 citation statements)

References 30 publications

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“…Aptamers are typically developed using the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method . The use of aptamers had led to the development of several electrochemical biosensing platforms toward various targets. − …”

Section: Introductionmentioning

confidence: 99%

Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Chergui

Rhili

Abrego-Martínez

et al. 2021

ACS Agric. Sci. Technol.

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The extensive use of the herbicide glyphosate has raised several health concerns due to the known toxicity of this chemical. The existing methods for glyphosate detection need better and more rapid accessibility for quick detection in an efficient manner. Here, we report an ultrasensitive aptamer-based electrochemical detection method for glyphosate. A high affinity single-stranded DNA (ssDNA) that binds specifically to glyphosate (GlyP) was selected and identified in vitro by the method of systemic evolution of ligands by exponential enrichment (SELEX) assisted by graphene oxide (GO), GO-SELEX. After eight GO-SELEX cycles, the ssDNA was sequenced, and the obtained aptamers were subjected to binding assays to evaluate their affinity and specificity to GlyP. The inherent GlyP–aptamer interaction was investigated by circular dichroism spectroscopy (CD). The selected sequence, denominated GlyP_1, exhibited a low dissociation constant (K d) of 30.73 ± 1.25 nM and excellent selectivity for glyphosate. Prior to gold electrode modification, GlyP_1 was functionalized by the disulfide label and used as a recognition element for glyphosate detection. The surface modification steps for the gold electrodes to develop the aptasensor were followed up by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of a redox probe, [Fe(CN)6]3–/4–. The resulting aptasensor demonstrated an operation range from 1.16 pM to 400 pM, with a limit of detection (LOD) of 1.16 pM. Thus, in this work we report an effective sensing method that could provide a potential alternative to traditional analyses techniques for quick and easy monitoring of glyphosate in food and water.

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Section: Introductionmentioning

confidence: 99%

Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Chergui

Rhili

Abrego-Martínez

et al. 2021

ACS Agric. Sci. Technol.

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show abstract

“…14 However, these methods are expensive, difficult, and time-consuming. 15 Compared with these technologies, electrochemical sensors have attracted much attention among researchers due to their low cost, fast response, good stability, and high sensitivity. 16,17 As we all know, electrode materials are the key factor affecting the analytical performance of electrochemical sensors.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Up to now, many methods for the determination of DA and ST have been reported, including spectrophotometry, fluorescence, high-performance liquid chromatography (HPLC), , mass spectrometry, capillary electrophoresis, and enzyme-linked immune sorbent assay (ELISA) . However, these methods are expensive, difficult, and time-consuming . Compared with these technologies, electrochemical sensors have attracted much attention among researchers due to their low cost, fast response, good stability, and high sensitivity. , As we all know, electrode materials are the key factor affecting the analytical performance of electrochemical sensors .…”

Section: Introductionmentioning

confidence: 99%

Gold Nanorods (AuNRs) and Zeolitic Imidazolate Framework-8 (ZIF-8) Core–Shell Nanostructure-Based Electrochemical Sensor for Detecting Neurotransmitters

et al. 2021

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The development of novel electrode materials for rapid and sensitive detection of neurotransmitters in the human body is of great significance for early disease diagnosis and personalized therapy. Herein, gold nanorod@zeolitic imidazolate framework-8 (AuNR@ZIF-8) core–shell nanostructures were prepared by controlled encapsulation of gold nanorods within a ZIF-8 assembly. The designed AuNR@ZIF-8 nanostructures have uniform morphology, good dispersion, a large specific surface area, and an average size of roughly 175 nm. Compared with individual ZIF-8 and AuNR-modified electrodes, the obtained core–shell-structured AuNR@ZIF-8 nanocomposite structure-modified electrode shows excellent electrocatalytic performance in the determination of dopamine (DA) and serotonin (ST). The designed AuNR@ZIF-8 exhibited a wide linear range of 0.1–50 μM and low detection limit (LOD, 0.03 μM, S/N = 3) for the determination of DA, as well as a linear range of 0.1–25 μM and low LOD (0.007 μM, S/N = 3) for monitoring ST. The improved performance is attributed to the synergistic effect of the high conductivity of AuNRs and multiple catalytic sites of ZIF-8. The good electroanalytical ability of AuNR@ZIF-8 for detection of DA and ST can provide a guide to efficiently and rapidly monitor other neurotransmitters and construct novel electrochemical sensors.

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“…In this reference, high‐affinity aptamers for 5‐HT (aptamer‐target dissociation constants, K d =30 nM) was selected and used for 5‐HT sensing. After that, several works for the determination of 5‐HT were reported [13–15]. Recently, one electrochemical aptasensor was reported for the determination of 5‐HT by immobilizing methylene blue (MB)‐labeled aptamers as capture and signal probe on the gold electrode [15].…”

Section: Introductionmentioning

confidence: 99%

“…After that, several works for the determination of 5‐HT were reported [13–15]. Recently, one electrochemical aptasensor was reported for the determination of 5‐HT by immobilizing methylene blue (MB)‐labeled aptamers as capture and signal probe on the gold electrode [15]. In this reference, two MB‐labeled aptamers with different labeling sites were used and good sensitivity was obtained for 5‐HT at aptasensor fabricated by intermediate‐labeled aptamer compared with terminal‐labeled aptamer.…”

Section: Introductionmentioning

confidence: 99%

Label‐free Electrochemical Aptasensor for the Determination of Serotonin

Rong

Liu

et al. 2021

Electroanalysis

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A label‐free electrochemical aptasensor was fabricated for the determination of serotonin (5‐HT) by the employment of specific binding between 5‐HT and aptamer and directly electrochemical measurement of 5‐HT oxidation. The comparison between two aptasensors and two strategies was studied. An electrochemical aptasensor fabricated using 57‐mer aptamer was facilely used to determine 5‐HT in the range from 1 μM to 100 μM with detection limit of 0.3 μM via one step recognition and detection. This work demonstrates that electrochemical method combing aptamer recognition and 5‐HT oxidation provides an alternative and promising method for the determination of 5‐HT with good selectivity and simplicity.

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A reusable neurotransmitter aptasensor for the sensitive detection of serotonin

Cited by 31 publications

References 30 publications

Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Selection of Highly Specific Aptamers by Graphene Oxide-SELEX to Ultrasensitive Label-Free Impedimetric Biosensor Development for Glyphosate Detection

Gold Nanorods (AuNRs) and Zeolitic Imidazolate Framework-8 (ZIF-8) Core–Shell Nanostructure-Based Electrochemical Sensor for Detecting Neurotransmitters

Label‐free Electrochemical Aptasensor for the Determination of Serotonin

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